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Literature summary for 3.2.1.20 extracted from

  • Bravo-Torres, J.C.; Calvo-Mendez, C.; Flores-Carreon, A.; Lopez-Romero, E.
    Purification and biochemical characterization of a soluble alpha-glucosidase from the parasite Entamoeba histolytica (2003), Antonie van Leeuwenhoek, 84, 169-178.
    View publication on PubMed
Search for more literature for 3.2.1.20

Inhibitors

Inhibitors Comment Organism Structure
1-deoxynojirimycin competitive, inhibitory potential depends on the substrate used Entamoeba histolytica
6-deoxy-D-glucose competitive to 4-methylumbelliferyl alpha-D-glucoside Entamoeba histolytica
australine competitive, inhibitory potential depends on the substrate used Entamoeba histolytica
castanospermine competitive, inhibitory potential depends on the substrate used Entamoeba histolytica
D-glucose competitive to 4-methylumbelliferyl alpha-D-glucoside Entamoeba histolytica
additional information competition level between inhibitors and substrates, overview Entamoeba histolytica

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Entamoeba histolytica
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 2 * 43000 + 2 * 68000, alpha2beta2, SDS-PAGE Entamoeba histolytica
68000
-
 2 * 43000 + 2 * 68000, alpha2beta2, SDS-PAGE Entamoeba histolytica
243000
-
 gel filtration Entamoeba histolytica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Entamoeba histolytica the enzyme has a role in the general carbohydrate metabolism, and in glycoprotein processing ?
-
 ?
additional information Entamoeba histolytica HM1:IMSS the enzyme has a role in the general carbohydrate metabolism, and in glycoprotein processing ?
-
 ?

Organism

Organism UniProt Comment Textmining
Entamoeba histolytica
-
-
-
Entamoeba histolytica HM1:IMSS
-
-
-

Purification (Commentary)

Purification (Comment) Organism
673fold by three chromatographical steps: ion exchange, gel filtration and adsorption chromatography Entamoeba histolytica

Source Tissue

Source Tissue Comment Organism Textmining
trophozoite
-
 Entamoeba histolytica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.36
-
 purified enzyme Entamoeba histolytica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl alpha-D-glucoside + H2O synthetic fluorogenic substrate, competitive to the other substrates Entamoeba histolytica 4-methylumbelliferol + alpha-D-glucose
-
 ?
isomaltose + H2O best substrate Entamoeba histolytica 2 alpha-D-glucose
-
 ?
isomaltose + H2O best substrate Entamoeba histolytica HM1:IMSS 2 alpha-D-glucose
-
 ?
maltose + H2O
-
 Entamoeba histolytica alpha-D-glucose + D-glucose
-
 ?
maltose + H2O
-
 Entamoeba histolytica HM1:IMSS alpha-D-glucose + D-glucose
-
 ?
additional information the enzyme has a role in the general carbohydrate metabolism, and in glycoprotein processing Entamoeba histolytica ?
-
 ?
additional information no activity with trehalose, kojibiose, and cellobiose Entamoeba histolytica ?
-
 ?
additional information the enzyme has a role in the general carbohydrate metabolism, and in glycoprotein processing Entamoeba histolytica HM1:IMSS ?
-
 ?
additional information no activity with trehalose, kojibiose, and cellobiose Entamoeba histolytica HM1:IMSS ?
-
 ?
nigerose + H2O
-
 Entamoeba histolytica 2 alpha-D-glucose
-
 ?
nigerose + H2O
-
 Entamoeba histolytica HM1:IMSS 2 alpha-D-glucose
-
 ?

Subunits

Subunits Comment Organism
tetramer 2 * 43000 + 2 * 68000, alpha2beta2, SDS-PAGE Entamoeba histolytica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 assay at Entamoeba histolytica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 assay at Entamoeba histolytica

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0012
-
 1-deoxynojirimycin versus isomaltose, pH 5.5, 45°C Entamoeba histolytica
0.0015
-
 castanospermine versus isomaltose, pH 5.5, 45°C Entamoeba histolytica
0.22
-
 6-deoxy-D-glucose versus 4-methylumbelliferyl alpha-D-glucoside, pH 5.5, 45°C Entamoeba histolytica
0.23
-
 D-glucose versus 4-methylumbelliferyl alpha-D-glucoside, pH 5.5, 45°C Entamoeba histolytica