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Literature summary for 3.2.1.20 extracted from
- Purification and characterization of an alpha-glucosidase from a hyperthermophilic archaebacterium, Pyrococcus furiosus, exhibiting a temperature optimum of 105 to 115¦C (1990), J. Bacteriol., 172, 3654-3660.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| guanidine hydrochloride | - |
Pyrococcus furiosus |  |
| maltose | inhibits hydrolysis of p-nitrophenyl-alpha-D-glucopyranoside | Pyrococcus furiosus | |
| SDS | significant but not complete loss of activity | Pyrococcus furiosus | |
| Urea | - |
Pyrococcus furiosus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.26 | - |
p-nitrophenyl-alpha-D-glucopyranoside | - |
Pyrococcus furiosus | |
| 0.77 | - |
methyl-alpha-D-glucopyranoside | - |
Pyrococcus furiosus | |
| 5.2 | - |
maltose | - |
Pyrococcus furiosus | |
| 8.2 | - |
isomaltose | - |
Pyrococcus furiosus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Pyrococcus furiosus | 5737 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus furiosus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pyrococcus furiosus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Pyrococcus furiosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isomaltose + H2O | - |
Pyrococcus furiosus | 2 alpha-D-glucose | - |
? | |
| maltose + H2O | - |
Pyrococcus furiosus | alpha-D-glucose + D-glucose | - |
? | |
| methyl-alpha-glucoside + H2O | - |
Pyrococcus furiosus | methanol + alpha-D-glucose | - |
? | |
| p-nitrophenyl-alpha-D-glucopyranoside + H2O | - |
Pyrococcus furiosus | 4-nitrophenol + D-glucose | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 105 | 115 | - |
Pyrococcus furiosus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 95 | 115 | 95°C: about 50% of maximal activity, 105-115°C: optimum | Pyrococcus furiosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 98 | - |
half-life: 48 h | Pyrococcus furiosus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6 | - |
Pyrococcus furiosus |
| 5.5 | - |
- |
Pyrococcus furiosus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 7.3 | pH 4.5: about 80% of maximal activity, pH 7.3: about 50% of maximal activity | Pyrococcus furiosus |
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