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Literature summary for 3.2.1.20 extracted from

  • Silva, C.P.; Terra, W.R.
    An alpha-glucosidase from perimicrovillar membranes of Dysdercus peruvianus (hemiptera: pyrrhocoridae) midgut cells. Purification and properties (1995), Insect Biochem. Mol. Biol., 25, 487-494.
No PubMed abstract available
Search for more literature for 3.2.1.20

Inhibitors

Inhibitors Comment Organism Structure
D-glucono-delta-lactone competitive Dysdercus peruvianus
erythritol
-
 Dysdercus peruvianus
Tris
-
 Dysdercus peruvianus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.86
-
 phenyl-alpha-D-glucoside
-
 Dysdercus peruvianus
1.4
-
 p-nitrophenyl alpha-D-glucoside
-
 Dysdercus peruvianus
2.6
-
 maltopentaose
-
 Dysdercus peruvianus
3.6
-
 p-aminophenyl alpha-D-glucoside
-
 Dysdercus peruvianus
7.6
-
 maltotetraose
-
 Dysdercus peruvianus
7.8
-
 maltotriose
-
 Dysdercus peruvianus
18.6
-
 sucrose
-
 Dysdercus peruvianus
19
-
 maltohexaose
-
 Dysdercus peruvianus
21
-
 maltoheptaose
-
 Dysdercus peruvianus
25
-
 turanose
-
 Dysdercus peruvianus
26
-
 maltose
-
 Dysdercus peruvianus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane perimicrovillar membranes Dysdercus peruvianus 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
 2 * 61000, SDS-PAGE Dysdercus peruvianus
120000
-
-
 Dysdercus peruvianus
130000
-
 ultracentrifugation Dysdercus peruvianus
431000
-
 non-denaturing PAGE Dysdercus peruvianus

Organism

Organism UniProt Comment Textmining
Dysdercus peruvianus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Dysdercus peruvianus

Purification (Commentary)

Purification (Comment) Organism
-
 Dysdercus peruvianus

Source Tissue

Source Tissue Comment Organism Textmining
midgut bound to perimicrovillar membranes Dysdercus peruvianus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7640
-
-
 Dysdercus peruvianus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltoheptaose + H2O
-
 Dysdercus peruvianus maltohexaose + D-glucose
-
 ?
maltohexaose + H2O
-
 Dysdercus peruvianus maltopentaose + D-glucose
-
 ?
maltopentaose + H2O
-
 Dysdercus peruvianus alpha-D-glucose + ?
-
 ?
maltose + H2O
-
 Dysdercus peruvianus alpha-D-glucose + D-glucose
-
 ?
maltotetraose + H2O
-
 Dysdercus peruvianus alpha-D-glucose
-
 ?
maltotriose + H2O
-
 Dysdercus peruvianus maltose + D-glucose
-
 ?
p-aminophenyl-alpha-D-glucoside + H2O
-
 Dysdercus peruvianus alpha-D-glucose + 4-aminophenol
-
 ?
p-nitrophenyl-alpha-D-glucopyranoside + H2O
-
 Dysdercus peruvianus 4-nitrophenol + D-glucose
-
 ?
phenyl-alpha-D-glucoside + H2O
-
 Dysdercus peruvianus phenol + alpha-D-glucose
-
 ?
sucrose + H2O
-
 Dysdercus peruvianus D-fructose + alpha-D-glucose
-
 ?
turanose + H2O
-
 Dysdercus peruvianus alpha-D-glucose + D-fructose
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 61000, SDS-PAGE Dysdercus peruvianus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.05
-
 maltopentaose
-
 Dysdercus peruvianus
3.42
-
 maltoheptaose
-
 Dysdercus peruvianus
3.77
-
 turanose
-
 Dysdercus peruvianus
3.97
-
 maltohexaose
-
 Dysdercus peruvianus
4.07
-
 sucrose
-
 Dysdercus peruvianus
4.67
-
 p-aminophenyl-alpha-D-glucoside
-
 Dysdercus peruvianus
5.22
-
 maltotetraose
-
 Dysdercus peruvianus
6.17
-
 maltotriose
-
 Dysdercus peruvianus
18
-
 maltose
-
 Dysdercus peruvianus
19
-
 p-Nitrophenyl-alpha-glucoside
-
 Dysdercus peruvianus
22.5
-
 phenyl-alpha-D-glucoside
-
 Dysdercus peruvianus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Dysdercus peruvianus