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Literature summary for 3.2.1.20 extracted from
- Purification and characterization of the cell-wall-associated and extracellular alpha-glucosidase from Saccharomyces fibuligera (1995), Biochem. J., 308, 753-760.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell wall | associated with | Saccharomycopsis fibuligera | 5618 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 132000 | - |
1 * 132000, SDS-PAGE | Saccharomycopsis fibuligera |
| 135000 | - |
gel filtration | Saccharomycopsis fibuligera |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomycopsis fibuligera | - |
KZ | - |
| Saccharomycopsis fibuligera KZ | - |
KZ | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | extracellular enzyme and cell-wall bound enzyme are glycoproteins. N-linked carbohydrate accounts for 12% of the total mass | Saccharomycopsis fibuligera |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomycopsis fibuligera |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| unable to renature after thermal inactivation, extracellular enzyme and cell-wall associated enzyme | Saccharomycopsis fibuligera |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 161 | - |
cell-wall-associated enzyme | Saccharomycopsis fibuligera |
| 175 | - |
extracellular enzyme | Saccharomycopsis fibuligera |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 24 h, pH 4.0-8.0, stable | Saccharomycopsis fibuligera |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isomaltose + H2O | - |
Saccharomycopsis fibuligera | 2 alpha-D-glucose | - |
? |  |
| isomaltose + H2O | - |
Saccharomycopsis fibuligera KZ | 2 alpha-D-glucose | - |
? | |
| maltose + H2O | - |
Saccharomycopsis fibuligera | alpha-D-glucose + D-glucose | - |
? | |
| maltose + H2O | - |
Saccharomycopsis fibuligera KZ | alpha-D-glucose + D-glucose | - |
? | |
| panose + H2O | - |
Saccharomycopsis fibuligera | maltose + alpha-D-glucose | - |
? | |
| panose + H2O | - |
Saccharomycopsis fibuligera KZ | maltose + alpha-D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 132000, SDS-PAGE | Saccharomycopsis fibuligera |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 43 | - |
cell-wall associated enzyme | Saccharomycopsis fibuligera |
| 52.5 | - |
extracellular enzyme | Saccharomycopsis fibuligera |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | - |
24 h, stable | Saccharomycopsis fibuligera |
| 30 | - |
cell-wall associated enzyme is stable up to, in absence of substrate | Saccharomycopsis fibuligera |
| 40 | - |
extracellular enzyme is stable up to | Saccharomycopsis fibuligera |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
extracellular enzyme and cell-wall bound enzyme | Saccharomycopsis fibuligera |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | 4°C, 24 h, stable | Saccharomycopsis fibuligera |
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