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Literature summary for 3.2.1.20 extracted from

  • Banno, Y.; Nozawa, Y.
    Purification and characterization of lysosomal alpha-glucosidase secreted by eukaryote Tetrahymena (1985), J. Biochem., 97, 409-418.
    View publication on PubMed
Search for more literature for 3.2.1.20

Inhibitors

Inhibitors Comment Organism Structure
turanose noncompetitive Tetrahymena pyriformis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1
-
 4-nitrophenyl alpha-glucopyranoside
-
 Tetrahymena pyriformis
2.5
-
 maltose
-
 Tetrahymena pyriformis
33
-
 isomaltose
-
 Tetrahymena pyriformis

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Tetrahymena pyriformis
-
-
lysosome
-
 Tetrahymena pyriformis 5764
-

Organism

Organism UniProt Comment Textmining
Tetrahymena pyriformis
-
-
-
Tetrahymena pyriformis W
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein contains 2.8% carbohydrate. The oligosaccharide moiety is composed of mannose and N-acetylglucosamine in a molar ratio of 6.7:2 Tetrahymena pyriformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-glucopyranoside + H2O
-
 Tetrahymena pyriformis 4-nitrophenol + alpha-glucopyranose
-
 ?
glycogen + H2O
-
 Tetrahymena pyriformis alpha-D-glucose
-
 ?
glycogen + H2O
-
 Tetrahymena pyriformis W alpha-D-glucose
-
 ?
isomaltose + H2O
-
 Tetrahymena pyriformis 2 alpha-D-glucose
-
 ?
isomaltose + H2O
-
 Tetrahymena pyriformis W 2 alpha-D-glucose
-
 ?
maltose + H2O
-
 Tetrahymena pyriformis alpha-D-glucose + D-glucose
-
 ?
maltose + H2O
-
 Tetrahymena pyriformis W alpha-D-glucose + D-glucose
-
 ?
additional information enzyme has alpha-1,6-glucosidase activity and alpha-1,4-glucosidase activity Tetrahymena pyriformis ?
-
 ?
additional information enzyme has alpha-1,6-glucosidase activity and alpha-1,4-glucosidase activity Tetrahymena pyriformis W ?
-
 ?
p-nitrophenyl-alpha-D-glucopyranoside + H2O p-nitrophenyl-alpha-D-glucopyranoside Tetrahymena pyriformis 4-nitrophenol + D-glucose
-
 ?
p-nitrophenyl-alpha-D-glucopyranoside + H2O p-nitrophenyl-alpha-D-glucopyranoside Tetrahymena pyriformis W 4-nitrophenol + D-glucose
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
 hydrolysis of maltose and p-nitrophenyl-alpha-glucopyranoside Tetrahymena pyriformis