Literature summary for 3.2.1.2 extracted from
Sagu, S.; Nso, E.; Homann, T.; Kapseu, C.; Rawel, H.
Isolation and purification of beta-amylase from stems of Cadaba farinosa and determination of its characteristics (2016), Curr. Top. Pept. Protein Res., 17, 21-35 .
No PubMed abstract available
Inhibitors
Inhibitors |
Comment |
Organism |
Structure |
acetic acid |
75% inhibition at 10 mM, 16.3% inhibition at 1 mM |
Cadaba farinosa |
|
Citric acid |
complete inhibition at 5-10 mM, 84.7% inhibition at 1 mM |
Cadaba farinosa |
|
CuSO4 |
over 94% inhibition at 1-10 mM |
Cadaba farinosa |
|
FeCl3 |
complete inhibition at 1-10 mM |
Cadaba farinosa |
|
Lactic acid |
complete inhibition at 10 mM, 58.3% inhibition at 1 mM |
Cadaba farinosa |
|
additional information |
no or poor effect by boric acid and urea at 1-10 mM |
Cadaba farinosa |
|
salicylic acid |
complete inhibition at 10 mM, 61.4% inhibition at 1 mM |
Cadaba farinosa |
|
Tannic acid |
complete inhibition at 10 mM, 81.8% inhibition at 1 mM |
Cadaba farinosa |
|
ZnSO4 |
15% inhibition at 10 mM, no inhibition at 1 mM |
Cadaba farinosa |
|
KM Value [mM]
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
additional information |
- |
additional information |
Michaelis-Menten kinetics and Lineweaver-Burk plots, Km and Vmax values at 50°C are estimated to be 2.81 mg/ml and 10.62 U/min, respectively |
Cadaba farinosa |
|
Metals/Ions
Metals/Ions |
Comment |
Organism |
Structure |
Ag+ |
activates 12% at 10 mM |
Cadaba farinosa |
|
Ca2+ |
activates 11% at 10 mM |
Cadaba farinosa |
|
K+ |
activates 10% at 10 mM |
Cadaba farinosa |
|
Na+ |
activates 11% at 10 mM |
Cadaba farinosa |
|
Natural Substrates/ Products (Substrates)
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
starch + H2O |
Cadaba farinosa |
beta-amylase is an exo-amylase acting on starch with the successive removal of maltose molecules from the non-reducing ends of the glucose polymers with inversion of the anomeric configuration |
? |
- |
? |
|
Organism
Organism |
UniProt |
Comment |
Textmining |
Cadaba farinosa |
- |
collected from Yagoua, a town of Mayo-Danay department, located in the Far North region, Cameroon |
- |
Purification (Commentary)
Purification (Comment) |
Organism |
native enzyme from powdered stems by three-phase partitioning (TPP), method evaluation and optimization, and model validation, overview |
Cadaba farinosa |
Source Tissue
Source Tissue |
Comment |
Organism |
Textmining |
stem |
- |
Cadaba farinosa |
- |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
starch + H2O |
beta-amylase is an exo-amylase acting on starch with the successive removal of maltose molecules from the non-reducing ends of the glucose polymers with inversion of the anomeric configuration |
Cadaba farinosa |
? |
- |
? |
|
Subunits
Subunits |
Comment |
Organism |
? |
x * 63000, SDS-PAGE |
Cadaba farinosa |
Temperature Optimum [°C]
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
50 |
- |
assay at |
Cadaba farinosa |
Temperature Range [°C]
Temperature Minimum [°C] |
Temperature Maximum [°C] |
Comment |
Organism |
50 |
70 |
high activity range |
Cadaba farinosa |
Temperature Stability [°C]
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
20 |
60 |
purified native enzyme, 1 h, pH 6.0, stable |
Cadaba farinosa |
80 |
- |
purified native enzyme, 1 h, pH 6.0, 80% activity remaining |
Cadaba farinosa |
90 |
- |
purified native enzyme, 1 h, pH 6.0, 5% activity remaining |
Cadaba farinosa |
pH Optimum
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
6.5 |
- |
- |
Cadaba farinosa |
pH Range
pH Minimum |
pH Maximum |
Comment |
Organism |
5 |
7 |
high activity range |
Cadaba farinosa |
pH Stability
pH Stability |
pH Stability Maximum |
Comment |
Organism |
5 |
7 |
purified native enzyme, 4°C, 4 h, over 65% activity remaining, rapid and almost complete loss of activity above |
Cadaba farinosa |