Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.2 extracted from

  • Zafar, A.; Aftab, M.; ud Din, Z.; Aftab, S.; Iqbal, I.; ul Haq, I.
    Cloning, purification and characterization of a highly Thermostable Amylase Gene of Thermotoga petrophila into Escherichia coli (2016), Appl. Biochem. Biotechnol., 178, 831-848 .
    View publication on PubMed
Search for more literature for 3.2.1.2

Application

Application Comment Organism
industry the recombinant purified alpha-amylase enzyme from Thermotoga petrophila can be a potential candidate for use in many industrial processes; particularly, it can be helpful in starch saccharification due to the adaptive capability of the alpha-amylase to withstand unreceptive conditions used in industrial processes Thermotoga petrophila

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3), pET 21a (+) vector Thermotoga petrophila

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 4%, 1h, activity is decreased to 53% Thermotoga petrophila
acetone 40%, relative activity is decreased to 87% Thermotoga petrophila
Co2+ 1 mM, 37% loss of activity Thermotoga petrophila
EDTA 1 mM, 42% loss of activity. 5-10 mM, complete loss of activity Thermotoga petrophila
Isopropanol 40%, relative activity is decreased to 80% Thermotoga petrophila
K+ 1 mM, 1% loss of activity Thermotoga petrophila
Mg2+ 1 mM, 14% loss of activity Thermotoga petrophila
additional information no considerable effect of organic solvents (ethanol, methanol, isopropanol, acetone and n-butanol) is observed on enzyme activity Thermotoga petrophila
n-butanol 40%, relative activity is decreased to 85% Thermotoga petrophila
Ni2+ 1 mM, 28% loss of activity Thermotoga petrophila
SDS 4%, 1 h, complete loss of activity Thermotoga petrophila
Tween 20 4%, 1h, activity is decreased to 71% Thermotoga petrophila
Tween 80 4%, 1h, activity is decreased to 61% Thermotoga petrophila

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activity is increased to 150 % in the presence of calcium chloride Thermotoga petrophila

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 SDS-PAGE Thermotoga petrophila

Organism

Organism UniProt Comment Textmining
Thermotoga petrophila A0A0U4KTC9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermotoga petrophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopektin + H2O
-
 Thermotoga petrophila ?
-
 ?
dextrin + H2O
-
 Thermotoga petrophila ?
-
 ?
soluble starch + H2O maximum activity with 1.2 % soluble starch. Less activity is observed with starch from corn, rice, glycogen Thermotoga petrophila maltose + ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
-
 Thermotoga petrophila

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 2 h, purified enzyme retains 100 % relative activity. Ater 4 h of incubation, the relative activity of the enzyme is 80% Thermotoga petrophila
60
-
 2 h, purified enzyme retains 100 % relative activity. Ater 4 h of incubation, the relative activity of the enzyme is 80% Thermotoga petrophila
70
-
 2 h, purified enzyme retains 100 % relative activity. Ater 4 h of incubation, the relative activity of the enzyme is 72% Thermotoga petrophila
80
-
 2 h, purified enzyme retains 100 % relative activity. Ater 4 h of incubation, the relative activity of the enzyme is 63% Thermotoga petrophila
90
-
 2 h, 15% loss of activity. Ater 4 h of incubation, the relative activity of the enzyme is 54% Thermotoga petrophila
100
-
 2 h, 22% loss of activity. Ater 4 h of incubation, the relative activity of the enzyme is 45% Thermotoga petrophila

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
 after 1 h of incubation, the relative activity of the enzyme is 20% Thermotoga petrophila
5
-
 after 1 h of incubation, the relative activity of the enzyme is 30% Thermotoga petrophila
6
-
 the enzyme exhibits 80% relative activity after 4 h Thermotoga petrophila
7
-
 maximum relative activity (100%) is obtained after 4 h at pH 7.0 Thermotoga petrophila
8
-
 the enzyme exhibits 70% relative activity after 3 h Thermotoga petrophila
9
-
 activity is decreased to 30% after 1 h of incubation Thermotoga petrophila