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Literature summary for 3.2.1.2 extracted from
- beta-Amylase in germinating millet seeds (2003), Phytochemistry, 64, 935-939.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 5 mM, 37°C, 30 min, about 80% inhibition | Panicum miliaceum |  |
| Hg2+ | 5 mM, 37°C, 30 min, about 80% inhibition | Panicum miliaceum | |
| Mn2+ | 5 mM, 37°C, 30 min, about 80% inhibition | Panicum miliaceum | |
| p-chloromercuribenzoate | 0.01 mM, 100% inhibition | Panicum miliaceum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
gel filtration | Panicum miliaceum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | Panicum miliaceum | beta-amylase should be a key enzyme in starch degradation during the germination of millet seeds, enzyme activity increases during days 1-4 of germination | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Panicum miliaceum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Panicum miliaceum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | germinating | Panicum miliaceum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 103.9 | - |
pH 4.5, 37°C, soluble starch | Panicum miliaceum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | 114.6% of the activity with amylose, soluble starch, amylose and amylopectin are the most suitable substrates, exo-hydrolase that releases beta-maltose from the non-reducing end of alpha-1,4-linked poly- and oligoglucans until the first alpha-1,6-branching point along the substrate molecule is encountered | Panicum miliaceum | ? | - |
? | |
| amylose + H2O | EX-I, soluble starch, amylose and amylopectin are the most suitable substrates, exo-hydrolase that releases beta-maltose from the non-reducing end of alpha-1,4-linked poly- and oligoglucans until the first alpha-1,6-branching point along the substrate molecule is encountered | Panicum miliaceum | ? | - |
? | |
| glycogen + H2O | 49.4% of the activity with amylose | Panicum miliaceum | ? | - |
? | |
| maltoheptaose + H2O | 34.6% of the activity with amylose | Panicum miliaceum | ? | - |
? | |
| maltohexaose + H2O | 23.3% of the activity with amylose | Panicum miliaceum | ? | - |
? | |
| maltooligosaccharide + H2O | beta-amylase hydrolyzes maltooligosaccharides more readily as their degree of polymerization increases, this being strongest for maltooligosaccharides larger than 13 glucose residues and very weakly for maltotriose, exo-hydrolase that releases beta-maltose from the non-reducing end of alpha-1,4-linked poly- and oligoglucans until the first alpha-1,6-branching point along the substrate molecule is encountered | Panicum miliaceum | ? | - |
? | |
| maltopentaose + H2O | 13.8% of the activity with amylose | Panicum miliaceum | ? | - |
? | |
| maltotetraose + H2O | 7.6% of the activity with amylose | Panicum miliaceum | ? | - |
? | |
| maltotriose + H2O | very poor substrate | Panicum miliaceum | ? | - |
? | |
| starch + H2O | beta-amylase should be a key enzyme in starch degradation during the germination of millet seeds, enzyme activity increases during days 1-4 of germination | Panicum miliaceum | ? | - |
? | |
| starch + H2O | 106.9% of the activity with amylose, soluble starch, amylose and amylopectin are the most suitable substrates, some activity against native starch, exo-hydrolase that releases beta-maltose from the non-reducing end of alpha-1,4-linked poly- and oligoglucans until the first alpha-1,6-branching point along the substrate molecule is encountered | Panicum miliaceum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 58000, SDS-PAGE | Panicum miliaceum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
optimum temperature after 30 min incubation | Panicum miliaceum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
15 min, 50 mM NaOAc buffer, pH 6, stable up to | Panicum miliaceum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 6 | - |
Panicum miliaceum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 9 | 15 h, 20°C, 50 mM McIlvaines buffer and sodium carbonate-boric acid buffer, stable in the range | Panicum miliaceum |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Panicum miliaceum | isoelectric focusing | - |
4.9 |
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