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Literature summary for 3.2.1.2 extracted from
- Expression in Escherichia coli of cDNA encoding barley beta-amylase and properties of recombinant beta-amylase (1994), Biosci. Biotechnol. Biochem., 58, 1080-1086.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli, the recombinant enzyme lacks 4 amino acids at positions 2-5 (Glu-Val-Asn-Val) when compared with barley beta-amylase | Hordeum vulgare |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 1 mM, almost complete inhibition of recombinant enzyme | Hordeum vulgare |  |
| Cd2+ | 1 mM, almost complete inhibition of recombinant enzyme | Hordeum vulgare | |
| Cu2+ | 1 mM, almost complete inhibition of recombinant enzyme | Hordeum vulgare | |
| Hg2+ | 1 mM, almost complete inhibition of recombinant enzyme | Hordeum vulgare | |
| PCMB | 0.1 mM, complete inhibition of recombinant enzyme | Hordeum vulgare | |
| Zn2+ | 1 mM, almost complete inhibition of recombinant enzyme | Hordeum vulgare | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Hordeum vulgare |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
x * 56000, recombinant enzyme, SDS-PAGE | Hordeum vulgare |
| 59169 | - |
x * 59169, recombinant enzyme, calculation from amino acid sequence | Hordeum vulgare |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hordeum vulgare | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| no glycoprotein | contains no carbohydrate | Hordeum vulgare |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Hordeum vulgare |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | - |
Hordeum vulgare | beta-maltose + ? | - |
? | |
| amylose + H2O | DP = 17 | Hordeum vulgare | beta-maltose | - |
? | |
| maltoheptaose + H2O | - |
Hordeum vulgare | ? | - |
? | |
| maltohexaose + H2O | - |
Hordeum vulgare | ? | - |
? | |
| maltopentaose + H2O | - |
Hordeum vulgare | ? | - |
? | |
| maltotetraose + H2O | - |
Hordeum vulgare | ? | - |
? | |
| starch + H2O | soluble | Hordeum vulgare | maltose + ? | beta-maltose | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 59169, recombinant enzyme, calculation from amino acid sequence | Hordeum vulgare |
| ? | x * 56000, recombinant enzyme, SDS-PAGE | Hordeum vulgare |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Hordeum vulgare |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 53 | - |
stable up to | Hordeum vulgare |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 7.5 | - |
Hordeum vulgare |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 9.5 | stable | Hordeum vulgare |
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