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Literature summary for 3.2.1.2 extracted from
- Purification and chemical characterization of thermostable amylases produced by Bacillus stearothermophilus (1987), Enzyme Microb. Technol., 9, 749-754.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Geobacillus stearothermophilus |  |
| Al3+ | mild inhibitor | Geobacillus stearothermophilus | |
| Cd2+ | - |
Geobacillus stearothermophilus | |
| EDTA | non-competitive | Geobacillus stearothermophilus | |
| Fe3+ | - |
Geobacillus stearothermophilus | |
| Hg2+ | - |
Geobacillus stearothermophilus | |
| iodoacetic acid | - |
Geobacillus stearothermophilus | |
| Mg2+ | mild inhibitor | Geobacillus stearothermophilus | |
| Mn2+ | mild inhibitor | Geobacillus stearothermophilus | |
| Ni2+ | - |
Geobacillus stearothermophilus | |
| Pb2+ | - |
Geobacillus stearothermophilus | |
| PCMB | - |
Geobacillus stearothermophilus | |
| Zn2+ | mild inhibitor | Geobacillus stearothermophilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km: 1.25 mg/ml for starch | Geobacillus stearothermophilus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | stimulates | Geobacillus stearothermophilus | |
| Ca2+ | stimulates | Geobacillus stearothermophilus | |
| K+ | stimulates | Geobacillus stearothermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57000 | - |
gel filtration | Geobacillus stearothermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Geobacillus stearothermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Geobacillus stearothermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Geobacillus stearothermophilus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | 90 | 85°C: 83% of maximal activity, 90°C: 32.8% of maximal activity | Geobacillus stearothermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.9 | - |
- |
Geobacillus stearothermophilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 10 | pH 5.5.: about 50% of maximal activity, pH 10.0: about 40% of maximal activity | Geobacillus stearothermophilus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 11.5 | 20°C, 24 h, stable. Sharp decrease in activity at pH 12.0 | Geobacillus stearothermophilus |
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