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Literature summary for 3.2.1.197 extracted from

  • Nihira, T.; Chiku, K.; Suzuki, E.; Nishimoto, M.; Fushinobu, S.; Kitaoka, M.; Ohtsubo, K.; Nakai, H.
    An inverting beta-1,2-mannosidase belonging to glycoside hydrolase family 130 from Dyadobacter fermentans (2015), FEBS Lett., 589, 3604-3610.
    View publication on PubMed
Search for more literature for 3.2.1.197

Cloned(Commentary)

Cloned (Comment) Organism
-
 Dyadobacter fermentans

Protein Variants

Protein Variants Comment Organism
E224A 1.9% of wild-type activity. Glutamic acid residues E265 and E224 are critical for the hydrolysis of beta-1,2-mannotriose. The residues are not conserved among GH130 phosphorylases and are predicted to assist the nucleophilic attack of a water molecule in the hydrolysis of the beta-D-mannosidic bond Dyadobacter fermentans
E265A 0.04% of wild-type activity. Glutamic acid residues E265 and E224 are critical for the hydrolysis of beta-1,2-mannotriose. The residues are not conserved among GH130 phosphorylases and are predicted to assist the nucleophilic attack of a water molecule in the hydrolysis of the beta-D-mannosidic bond Dyadobacter fermentans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.56
-
 beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose pH 5.5, 30°C Dyadobacter fermentans
0.95
-
 beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose pH 5.5, 30°C Dyadobacter fermentans
15
-
 beta-D-mannopyranosyl-(1->2)-D-mannopyranose pH 5.5, 30°C Dyadobacter fermentans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + H2O Dyadobacter fermentans
-
 beta-D-mannopyranosyl-(1->2)-D-mannopyranose + alpha-D-mannopyranose enzyme releases alpha-D-mannose from beta-1,2-mannooligosaccharides with an inversion of the anomeric configuration ?
beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + H2O Dyadobacter fermentans DSM 18053
-
 beta-D-mannopyranosyl-(1->2)-D-mannopyranose + alpha-D-mannopyranose enzyme releases alpha-D-mannose from beta-1,2-mannooligosaccharides with an inversion of the anomeric configuration ?

Organism

Organism UniProt Comment Textmining
Dyadobacter fermentans C6W7B1
-
-
Dyadobacter fermentans DSM 18053 C6W7B1
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.015
-
 mutant E265A, pH 5.5, 30°C Dyadobacter fermentans
0.71
-
 mutant E224A, pH 5.5, 30°C Dyadobacter fermentans
37
-
 wild-type, pH 5.5, 30°C Dyadobacter fermentans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + H2O i.e. beta-1,2-mannotetraose Dyadobacter fermentans beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + alpha-D-mannopyranose
-
 ?
beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + H2O i.e. beta-1,2-mannotetraose Dyadobacter fermentans DSM 18053 beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + alpha-D-mannopyranose
-
 ?
beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + H2O
-
 Dyadobacter fermentans beta-D-mannopyranosyl-(1->2)-D-mannopyranose + alpha-D-mannopyranose enzyme releases alpha-D-mannose from beta-1,2-mannooligosaccharides with an inversion of the anomeric configuration ?
beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + H2O i.e. beta-1,2-mannotriose Dyadobacter fermentans beta-D-mannopyranosyl-(1->2)-D-mannopyranose + alpha-D-mannopyranose enzyme releases alpha-D-mannose from beta-1,2-mannooligosaccharides with an inversion of the anomeric configuration ?
beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + H2O
-
 Dyadobacter fermentans DSM 18053 beta-D-mannopyranosyl-(1->2)-D-mannopyranose + alpha-D-mannopyranose enzyme releases alpha-D-mannose from beta-1,2-mannooligosaccharides with an inversion of the anomeric configuration ?
beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + H2O i.e. beta-1,2-mannotriose Dyadobacter fermentans DSM 18053 beta-D-mannopyranosyl-(1->2)-D-mannopyranose + alpha-D-mannopyranose enzyme releases alpha-D-mannose from beta-1,2-mannooligosaccharides with an inversion of the anomeric configuration ?
beta-D-mannopyranosyl-(1->2)-D-mannopyranose + H2O i.e. beta-1,2-mannobiose Dyadobacter fermentans beta-D-mannopyranose + alpha-D-mannopyranose
-
 ?
beta-D-mannopyranosyl-(1->2)-D-mannopyranose + H2O i.e. beta-1,2-mannobiose Dyadobacter fermentans DSM 18053 beta-D-mannopyranose + alpha-D-mannopyranose
-
 ?

Synonyms

Synonyms Comment Organism
Dfer_3176
-
 Dyadobacter fermentans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 stable up to for 15 min Dyadobacter fermentans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
26
-
 beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose pH 5.5, 30°C Dyadobacter fermentans
30
-
 beta-D-mannopyranosyl-(1->2)-D-mannopyranose pH 5.5, 30°C Dyadobacter fermentans
30
-
 beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose pH 5.5, 30°C Dyadobacter fermentans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Dyadobacter fermentans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2
-
 beta-D-mannopyranosyl-(1->2)-D-mannopyranose pH 5.5, 30°C Dyadobacter fermentans
32
-
 beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose pH 5.5, 30°C Dyadobacter fermentans
46
-
 beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose pH 5.5, 30°C Dyadobacter fermentans