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Literature summary for 3.2.1.196 extracted from
- The glgX gene product of Corynebacterium glutamicum is required for glycogen degradation and for fast adaptation to hyperosmotic stress (2007), Microbiology, 153, 2212-2220.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli ER2566 cells | Corynebacterium glutamicum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 110000 | - |
4 * 110000, His6-tagged enzyme, SDS-PAGE | Corynebacterium glutamicum |
| 417000 | - |
native enzyme, gel filtration | Corynebacterium glutamicum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphorylase-limit dextrin | Corynebacterium glutamicum | - |
maltodextrin + ? | - |
? |  |
| phosphorylase-limit dextrin | Corynebacterium glutamicum ATCC 13032 | - |
maltodextrin + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Corynebacterium glutamicum | - |
- |
- |
| Corynebacterium glutamicum ATCC 13032 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HisTrap column chromatography and Superdex 200 gel filtration | Corynebacterium glutamicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | - |
Corynebacterium glutamicum | maltose | - |
? | |
| amylopectin + H2O | - |
Corynebacterium glutamicum ATCC 13032 | maltose | - |
? | |
| glycogen + H2O | - |
Corynebacterium glutamicum | maltose + ? | - |
? | |
| glycogen + H2O | - |
Corynebacterium glutamicum ATCC 13032 | maltose + ? | - |
? | |
| phosphorylase-limit dextrin | - |
Corynebacterium glutamicum | maltodextrin + ? | - |
? | |
| phosphorylase-limit dextrin | - |
Corynebacterium glutamicum ATCC 13032 | maltodextrin + ? | - |
? | |
| soluble starch + H2O | - |
Corynebacterium glutamicum | maltose + ? | - |
? | |
| soluble starch + H2O | - |
Corynebacterium glutamicum ATCC 13032 | maltose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 110000, His6-tagged enzyme, SDS-PAGE | Corynebacterium glutamicum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GlgX | - |
Corynebacterium glutamicum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 95 | - |
the His6-tagged enzyme is inactivated by incubation at 95°C for 10 min | Corynebacterium glutamicum |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | inactivation of glgX leads to slower growth and to a higher intracellular glycogen pool throughout growth, when compared to those in the parental strain | Corynebacterium glutamicum |
| physiological function | the enzyme is required for glycogen degradation and for fast adaptation to hyperosmotic stress | Corynebacterium glutamicum |
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