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Literature summary for 3.2.1.196 extracted from

  • Song, H.N.; Jung, T.Y.; Park, J.T.; Park, B.C.; Myung, P.K.; Boos, W.; Woo, E.J.; Park, K.H.
    Structural rationale for the short branched substrate specificity of the glycogen debranching enzyme GlgX (2010), Proteins, 78, 1847-1855.
    View publication on PubMed
Search for more literature for 3.2.1.196

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli MC1061 cells Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.2 M sodium citrate, 47% (v/v) MPD, 4% (v/v) PEG 3350, and HEPES (pH 8.0) buffer Escherichia coli
hanging drop vapor diffusion method, using 0.2 M sodium citrate, 47% (w/v) 2-methyl-2,4-pentanediol, 4% (w/v) PEG 3350, and HEPES (pH 8.0) buffer Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
 G4-beta-cyclodextrin in 50 mM sodium phosphate buffer (pH7.0) at 37°C Escherichia coli
1.51
-
 G3-beta-cyclodextrin in 50 mM sodium phosphate buffer (pH7.0) at 37°C Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphorylase-limit dextrin + H2O Escherichia coli the enzyme hydrolyzes alpha-1,6-glycosidic linkages of phosphorylase-limit dextrin containing only three or four glucose subunits ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli P15067
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 200 gel filtration Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
G3-beta-cyclodextrin + H2O
-
 Escherichia coli ?
-
 ?
G4-beta-cyclodextrin + H2O
-
 Escherichia coli ?
-
 ?
G4-beta-cyclodextrin + H2O GlgX shows substrate specificity for G4 phosphorylase-limit dextrin Escherichia coli ?
-
 ?
additional information no activity towards G2-beta-cyclodextrin and G5-beta-cyclodextrin Escherichia coli ?
-
 ?
additional information no activity with G2- and G5-beta-cyclodextrin Escherichia coli ?
-
 ?
phosphorylase-limit dextrin + H2O the enzyme hydrolyzes alpha-1,6-glycosidic linkages of phosphorylase-limit dextrin containing only three or four glucose subunits Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
GDE
-
 Escherichia coli
GlgX
-
 Escherichia coli
glycogen debranching enzyme
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
38
-
 G3-beta-cyclodextrin in 50 mM sodium phosphate buffer (pH7.0) at 37°C Escherichia coli
41.7
-
 G4-beta-cyclodextrin in 50 mM sodium phosphate buffer (pH7.0) at 37°C Escherichia coli