BRENDA - Enzyme Database show
show all sequences of 3.2.1.195

Characterization of a novel arabinose-tolerant alpha-L-arabinofuranosidase with high ginsenoside Rc to ginsenoside Rd bioconversion productivity

Xie, J.; Zhao, D.; Zhao, L.; Pei, J.; Xiao, W.; Ding, G.; Wang, Z.; Xu, J.; J. Appl. Microbiol. 120, 647-660 (2016)

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
in a reaction at 85C and pH 5.0, 25 g/l of ginsenoside Rc is transformed into 21.8 g/l of ginsenoside Rd within 60 min, with a corresponding molar conversion of 99.4% and a high ginsenoside Rd productivity of 21800 mg/l/h
Pseudothermotoga thermarum
Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Pseudothermotoga thermarum
Inhibitors
Inhibitors
Commentary
Organism
Structure
Hg2+
1 mM, no residual activtiy
Pseudothermotoga thermarum
additional information
not inhibitory at 1 mM: EDTA
Pseudothermotoga thermarum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
55000
-
x * 55000, SDS-PAGE
Pseudothermotoga thermarum
Organic Solvent Stability
Organic Solvent
Commentary
Organism
DMSO
10% v/v, 69% residual activity
Pseudothermotoga thermarum
Ethanol
10% v/v, 71% residual activity
Pseudothermotoga thermarum
Methanol
10% v/v, 56% residual activity
Pseudothermotoga thermarum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudothermotoga thermarum
F7YTI3
-
-
Pseudothermotoga thermarum DSM 5069
F7YTI3
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ginsenoside Rc + H2O
-
738475
Pseudothermotoga thermarum
ginsenoside Rd + L-arabinofuranose
-
-
-
?
ginsenoside Rc + H2O
-
738475
Pseudothermotoga thermarum DSM 5069
ginsenoside Rd + L-arabinofuranose
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 55000, SDS-PAGE
Pseudothermotoga thermarum
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
95
-
-
Pseudothermotoga thermarum
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
75
100
more than 50% of maximum activtiy
Pseudothermotoga thermarum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
-
Pseudothermotoga thermarum
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
4
7.5
stable
Pseudothermotoga thermarum
Application (protein specific)
Application
Commentary
Organism
synthesis
in a reaction at 85C and pH 5.0, 25 g/l of ginsenoside Rc is transformed into 21.8 g/l of ginsenoside Rd within 60 min, with a corresponding molar conversion of 99.4% and a high ginsenoside Rd productivity of 21800 mg/l/h
Pseudothermotoga thermarum
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Pseudothermotoga thermarum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Hg2+
1 mM, no residual activtiy
Pseudothermotoga thermarum
additional information
not inhibitory at 1 mM: EDTA
Pseudothermotoga thermarum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
55000
-
x * 55000, SDS-PAGE
Pseudothermotoga thermarum
Organic Solvent Stability (protein specific)
Organic Solvent
Commentary
Organism
DMSO
10% v/v, 69% residual activity
Pseudothermotoga thermarum
Ethanol
10% v/v, 71% residual activity
Pseudothermotoga thermarum
Methanol
10% v/v, 56% residual activity
Pseudothermotoga thermarum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ginsenoside Rc + H2O
-
738475
Pseudothermotoga thermarum
ginsenoside Rd + L-arabinofuranose
-
-
-
?
ginsenoside Rc + H2O
-
738475
Pseudothermotoga thermarum DSM 5069
ginsenoside Rd + L-arabinofuranose
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 55000, SDS-PAGE
Pseudothermotoga thermarum
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
95
-
-
Pseudothermotoga thermarum
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
75
100
more than 50% of maximum activtiy
Pseudothermotoga thermarum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
-
Pseudothermotoga thermarum
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
4
7.5
stable
Pseudothermotoga thermarum
Other publictions for EC 3.2.1.195
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738475
Xie
Characterization of a novel ar ...
Pseudothermotoga thermarum, Pseudothermotoga thermarum DSM 5069
J. Appl. Microbiol.
120
647-660
2016
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723612
Yu
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A new ginsenosidase from Asper ...
Aspergillus sp., Aspergillus sp. g48p
Proc. Biochem.
44
772-775
2009
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