BRENDA - Enzyme Database
show all sequences of 3.2.1.193

Preparation of minor ginsenosides C-Mx and C-K from notoginseng leaf ginsenosides by a special ginsenosidase type-I

Liu, C.; Zuo, K.; Yu, H.; Sun, C.; Zhang, T.; Xu, L.; Jin, Y.; Im, W.; Jin, F.; Process Biochem. 50, 2158-2167 (2015)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
Vmax is 79.6 mM/h for Rb1, 45.6 mM/h for Rb2, 1.62 mM/h for Rb3 to Rd, 10.8 mM/h for Rb3 to C-Mx1, and 6.16 mM/h for Rc
Aspergillus niger
5.46
-
ginsenoside Rc
pH 5.0, 45°C
Aspergillus niger
15.1
-
ginsenoside Rb3
pH 5.0, 45°C, formation of compound Mx-1
Aspergillus niger
16.6
-
ginsenoside Rb1
pH 5.0, 45°C
Aspergillus niger
16.6
-
ginsenoside Rb3
pH 5.0, 45°C, formation of ginsenoside Rd
Aspergillus niger
20.4
-
ginsenoside Rb2
pH 5.0, 45°C
Aspergillus niger
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
compound Mc + H2O
Aspergillus niger
-
compound K + L-arabinofuranose
-
-
?
compound Mc1 + H2O
Aspergillus niger
-
compound Mc + D-glucose
-
-
?
compound Mx + H2O
Aspergillus niger
-
compound K + D-xylose
-
-
?
compound Mx-1 + H2O
Aspergillus niger
-
compound Mx + D-glucose
-
-
?
compound O + H2O
Aspergillus niger
-
compound Y + D-glucose
-
-
?
compound Y + H2O
Aspergillus niger
-
compound K + L-arabinopyranose
-
-
?
ginsenoside F2 + H2O
Aspergillus niger
-
compound K + D-glucose
-
-
?
ginsenoside Rb2 + H2O
Aspergillus niger
-
compound O + D-glucose
-
-
?
ginsenoside Rb3 + H2O
Aspergillus niger
-
ginsenoside Rd + D-xylose
-
-
?
ginsenoside Rb3 + H2O
Aspergillus niger
-
compound Mx1 + D-glucose
-
-
?
ginsenoside Rb3 + H2O
Aspergillus niger g.848
-
ginsenoside Rd + D-xylose
-
-
?
ginsenoside Rc + H2O
Aspergillus niger
-
ginsenoside Rd + L-arabinofuranose
-
-
?
ginsenoside Rc + H2O
Aspergillus niger
-
compound Mc1 + D-glucose
-
-
?
ginsenoside Rc + H2O
Aspergillus niger g.848
-
ginsenoside Rd + L-arabinofuranose
-
-
?
ginsenoside Rd + H2O
Aspergillus niger
-
ginsenoside F2 + D-glucose
-
-
?
additional information
Aspergillus niger
substrates are notoginseng leaf ginsenosides
?
-
-
?
additional information
Aspergillus niger g.848
substrates are notoginseng leaf ginsenosides
?
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Aspergillus niger
-
-
-
Aspergillus niger g.848
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
native enzyme from mycelium by anion exchange chromatography to homogeneity
Aspergillus niger
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
compound Mc + H2O
-
755274
Aspergillus niger
compound K + L-arabinofuranose
-
-
-
?
compound Mc1 + H2O
-
755274
Aspergillus niger
compound Mc + D-glucose
-
-
-
?
compound Mx + H2O
-
755274
Aspergillus niger
compound K + D-xylose
-
-
-
?
compound Mx-1 + H2O
-
755274
Aspergillus niger
compound Mx + D-glucose
-
-
-
?
compound O + H2O
-
755274
Aspergillus niger
compound Y + D-glucose
-
-
-
?
compound Y + H2O
-
755274
Aspergillus niger
compound K + L-arabinopyranose
-
-
-
?
ginsenoside F2 + H2O
-
755274
Aspergillus niger
compound K + D-glucose
-
-
-
?
ginsenoside Rb1 + H2O
-
755274
Aspergillus niger
ginsenoside Rd + D-glucose
-
-
-
?
ginsenoside Rb1 + H2O
-
755274
Aspergillus niger g.848
ginsenoside Rd + D-glucose
-
-
-
?
ginsenoside Rb2 + H2O
-
755274
Aspergillus niger
compound O + D-glucose
-
-
-
?
ginsenoside Rb3 + H2O
-
755274
Aspergillus niger
ginsenoside Rd + D-xylose
-
-
-
?
ginsenoside Rb3 + H2O
-
755274
Aspergillus niger g.848
ginsenoside Rd + D-xylose
-
-
-
?
ginsenoside Rb3 + H2O
-
755274
Aspergillus niger
compound Mx1 + D-glucose
-
-
-
?
ginsenoside Rc + H2O
-
755274
Aspergillus niger
ginsenoside Rd + L-arabinofuranose
-
-
-
?
ginsenoside Rc + H2O
-
755274
Aspergillus niger g.848
ginsenoside Rd + L-arabinofuranose
-
-
-
?
ginsenoside Rc + H2O
-
755274
Aspergillus niger
compound Mc1 + D-glucose
-
-
-
?
ginsenoside Rd + H2O
-
755274
Aspergillus niger
ginsenoside F2 + D-glucose
-
-
-
?
additional information
substrates are notoginseng leaf ginsenosides
755274
Aspergillus niger
?
-
-
-
?
additional information
the enzyme first hydrolyzes the C-20 position 20-O-Glc of ginsenoside Rb1, and then C-3 position 3-O-Glc via a single pathway: Rb1 -> Rd -> F2 -> C-K. It first hydrolyzes 3-O-Glc of ginsenoside Rb2 and Rc, and then 20-O-Ara via a single pathway: Rb2 -> C-O -> C-Y -> C-K and Rc -> C-Mc1 -> C-Mc -> C-K. It mainly hydrolyzes 3-O-Glc of Rb3 via pathway: Rb3 -> C-Mx1 -> C-Mx -> C-K, and slowly hydrolyzes 20-O-Xyl of Rb3 via pathway Rb3 -> Rd -> F2 -> C-K. The average mole yields are 48.2% for compound Mx from ginsenoside Rb3 and compound Mx1, and 41.2% for compound K from other ginsenosides. Products are identified by by TLC and HPLC analysis and NMR spectroscopy. The ability of the pure enzyme to hydrolyze 3-O- and 20-O-glucoside proves that it is a ginsenosidase type-I
755274
Aspergillus niger
?
-
-
-
?
additional information
substrates are notoginseng leaf ginsenosides
755274
Aspergillus niger g.848
?
-
-
-
?
additional information
the enzyme first hydrolyzes the C-20 position 20-O-Glc of ginsenoside Rb1, and then C-3 position 3-O-Glc via a single pathway: Rb1 -> Rd -> F2 -> C-K. It first hydrolyzes 3-O-Glc of ginsenoside Rb2 and Rc, and then 20-O-Ara via a single pathway: Rb2 -> C-O -> C-Y -> C-K and Rc -> C-Mc1 -> C-Mc -> C-K. It mainly hydrolyzes 3-O-Glc of Rb3 via pathway: Rb3 -> C-Mx1 -> C-Mx -> C-K, and slowly hydrolyzes 20-O-Xyl of Rb3 via pathway Rb3 -> Rd -> F2 -> C-K. The average mole yields are 48.2% for compound Mx from ginsenoside Rb3 and compound Mx1, and 41.2% for compound K from other ginsenosides. Products are identified by by TLC and HPLC analysis and NMR spectroscopy. The ability of the pure enzyme to hydrolyze 3-O- and 20-O-glucoside proves that it is a ginsenosidase type-I
755274
Aspergillus niger g.848
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 75000, SDS-PAGE
Aspergillus niger
Synonyms
Synonyms
Commentary
Organism
ginsenosidase type-I
-
Aspergillus niger
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
45
-
-
Aspergillus niger
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
-
Aspergillus niger
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
Vmax is 79.6 mM/h for Rb1, 45.6 mM/h for Rb2, 1.62 mM/h for Rb3 to Rd, 10.8 mM/h for Rb3 to C-Mx1, and 6.16 mM/h for Rc
Aspergillus niger
5.46
-
ginsenoside Rc
pH 5.0, 45°C
Aspergillus niger
15.1
-
ginsenoside Rb3
pH 5.0, 45°C, formation of compound Mx-1
Aspergillus niger
16.6
-
ginsenoside Rb1
pH 5.0, 45°C
Aspergillus niger
16.6
-
ginsenoside Rb3
pH 5.0, 45°C, formation of ginsenoside Rd
Aspergillus niger
20.4
-
ginsenoside Rb2
pH 5.0, 45°C
Aspergillus niger
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
compound Mc + H2O
Aspergillus niger
-
compound K + L-arabinofuranose
-
-
?
compound Mc1 + H2O
Aspergillus niger
-
compound Mc + D-glucose
-
-
?
compound Mx + H2O
Aspergillus niger
-
compound K + D-xylose
-
-
?
compound Mx-1 + H2O
Aspergillus niger
-
compound Mx + D-glucose
-
-
?
compound O + H2O
Aspergillus niger
-
compound Y + D-glucose
-
-
?
compound Y + H2O
Aspergillus niger
-
compound K + L-arabinopyranose
-
-
?
ginsenoside F2 + H2O
Aspergillus niger
-
compound K + D-glucose
-
-
?
ginsenoside Rb2 + H2O
Aspergillus niger
-
compound O + D-glucose
-
-
?
ginsenoside Rb3 + H2O
Aspergillus niger
-
ginsenoside Rd + D-xylose
-
-
?
ginsenoside Rb3 + H2O
Aspergillus niger
-
compound Mx1 + D-glucose
-
-
?
ginsenoside Rb3 + H2O
Aspergillus niger g.848
-
ginsenoside Rd + D-xylose
-
-
?
ginsenoside Rc + H2O
Aspergillus niger
-
ginsenoside Rd + L-arabinofuranose
-
-
?
ginsenoside Rc + H2O
Aspergillus niger
-
compound Mc1 + D-glucose
-
-
?
ginsenoside Rc + H2O
Aspergillus niger g.848
-
ginsenoside Rd + L-arabinofuranose
-
-
?
ginsenoside Rd + H2O
Aspergillus niger
-
ginsenoside F2 + D-glucose
-
-
?
additional information
Aspergillus niger
substrates are notoginseng leaf ginsenosides
?
-
-
?
additional information
Aspergillus niger g.848
substrates are notoginseng leaf ginsenosides
?
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
native enzyme from mycelium by anion exchange chromatography to homogeneity
Aspergillus niger
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
compound Mc + H2O
-
755274
Aspergillus niger
compound K + L-arabinofuranose
-
-
-
?
compound Mc1 + H2O
-
755274
Aspergillus niger
compound Mc + D-glucose
-
-
-
?
compound Mx + H2O
-
755274
Aspergillus niger
compound K + D-xylose
-
-
-
?
compound Mx-1 + H2O
-
755274
Aspergillus niger
compound Mx + D-glucose
-
-
-
?
compound O + H2O
-
755274
Aspergillus niger
compound Y + D-glucose
-
-
-
?
compound Y + H2O
-
755274
Aspergillus niger
compound K + L-arabinopyranose
-
-
-
?
ginsenoside F2 + H2O
-
755274
Aspergillus niger
compound K + D-glucose
-
-
-
?
ginsenoside Rb1 + H2O
-
755274
Aspergillus niger
ginsenoside Rd + D-glucose
-
-
-
?
ginsenoside Rb1 + H2O
-
755274
Aspergillus niger g.848
ginsenoside Rd + D-glucose
-
-
-
?
ginsenoside Rb2 + H2O
-
755274
Aspergillus niger
compound O + D-glucose
-
-
-
?
ginsenoside Rb3 + H2O
-
755274
Aspergillus niger
ginsenoside Rd + D-xylose
-
-
-
?
ginsenoside Rb3 + H2O
-
755274
Aspergillus niger g.848
ginsenoside Rd + D-xylose
-
-
-
?
ginsenoside Rb3 + H2O
-
755274
Aspergillus niger
compound Mx1 + D-glucose
-
-
-
?
ginsenoside Rc + H2O
-
755274
Aspergillus niger
ginsenoside Rd + L-arabinofuranose
-
-
-
?
ginsenoside Rc + H2O
-
755274
Aspergillus niger g.848
ginsenoside Rd + L-arabinofuranose
-
-
-
?
ginsenoside Rc + H2O
-
755274
Aspergillus niger
compound Mc1 + D-glucose
-
-
-
?
ginsenoside Rd + H2O
-
755274
Aspergillus niger
ginsenoside F2 + D-glucose
-
-
-
?
additional information
substrates are notoginseng leaf ginsenosides
755274
Aspergillus niger
?
-
-
-
?
additional information
the enzyme first hydrolyzes the C-20 position 20-O-Glc of ginsenoside Rb1, and then C-3 position 3-O-Glc via a single pathway: Rb1 -> Rd -> F2 -> C-K. It first hydrolyzes 3-O-Glc of ginsenoside Rb2 and Rc, and then 20-O-Ara via a single pathway: Rb2 -> C-O -> C-Y -> C-K and Rc -> C-Mc1 -> C-Mc -> C-K. It mainly hydrolyzes 3-O-Glc of Rb3 via pathway: Rb3 -> C-Mx1 -> C-Mx -> C-K, and slowly hydrolyzes 20-O-Xyl of Rb3 via pathway Rb3 -> Rd -> F2 -> C-K. The average mole yields are 48.2% for compound Mx from ginsenoside Rb3 and compound Mx1, and 41.2% for compound K from other ginsenosides. Products are identified by by TLC and HPLC analysis and NMR spectroscopy. The ability of the pure enzyme to hydrolyze 3-O- and 20-O-glucoside proves that it is a ginsenosidase type-I
755274
Aspergillus niger
?
-
-
-
?
additional information
substrates are notoginseng leaf ginsenosides
755274
Aspergillus niger g.848
?
-
-
-
?
additional information
the enzyme first hydrolyzes the C-20 position 20-O-Glc of ginsenoside Rb1, and then C-3 position 3-O-Glc via a single pathway: Rb1 -> Rd -> F2 -> C-K. It first hydrolyzes 3-O-Glc of ginsenoside Rb2 and Rc, and then 20-O-Ara via a single pathway: Rb2 -> C-O -> C-Y -> C-K and Rc -> C-Mc1 -> C-Mc -> C-K. It mainly hydrolyzes 3-O-Glc of Rb3 via pathway: Rb3 -> C-Mx1 -> C-Mx -> C-K, and slowly hydrolyzes 20-O-Xyl of Rb3 via pathway Rb3 -> Rd -> F2 -> C-K. The average mole yields are 48.2% for compound Mx from ginsenoside Rb3 and compound Mx1, and 41.2% for compound K from other ginsenosides. Products are identified by by TLC and HPLC analysis and NMR spectroscopy. The ability of the pure enzyme to hydrolyze 3-O- and 20-O-glucoside proves that it is a ginsenosidase type-I
755274
Aspergillus niger g.848
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 75000, SDS-PAGE
Aspergillus niger
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
45
-
-
Aspergillus niger
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
-
Aspergillus niger
Other publictions for EC 3.2.1.193
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
752455
Upadhyaya
Purification and characteriza ...
Armillaria mellea, Ganoderma lucidum, Pleurotus ostreatus, Ganoderma applanatum, Sanghuangporus baumii, Ganoderma lucidum KACC 42231, Armillaria mellea KACC 50013, Pleurotus ostreatus KACC 50356, Ganoderma applanatum KACC 53688, Sanghuangporus baumii KACC 53719
AMB Express
6
112
2016
-
-
-
-
-
-
-
-
-
-
-
17
-
10
-
-
1
-
-
5
1
-
31
1
6
2
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
17
-
-
-
1
-
5
1
-
31
1
2
-
-
-
1
-
-
-
-
1
1
-
-
-
753334
Shin
Classification of glycosidase ...
Aspergillus niger, Aspergillus oryzae, Aspergillus sp., Microbacterium esteraromaticum, Leuconostoc mesenteroides, Citrus limon, Lactiplantibacillus pentosus, Leuconostoc citreum, Byssochlamys spectabilis, Sulfolobus acidocaldarius, Saccharolobus solfataricus, Fusobacterium sp., Pseudarthrobacter chlorophenolicus, Intrasporangium sp., Globisporangium irregulare, Nocardioides panaciterrulae, Companilactobacillus paralimentarius, Sphingomonas leidyi, Byssochlamys spectabilis 229-7, Lactiplantibacillus pentosus 6105, Intrasporangium sp. GS603, Leuconostoc mesenteroides DC102, Leuconostoc citreum LH1, Aspergillus niger g.848
Crit. Rev. Biotechnol.
36
1036-1049
2016
-
-
-
-
-
-
-
-
-
-
-
77
-
25
-
-
-
-
-
-
3
-
77
7
27
7
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
77
-
-
-
-
-
-
3
-
77
7
7
-
-
-
7
-
-
-
-
5
5
-
-
-
753956
Li
Production of ginsenoside F2 ...
Paenibacillus mucilaginosus, Paenibacillus mucilaginosus KCTC 3870
J. Agric. Food Chem.
64
2506-2512
2016
-
1
1
-
-
-
-
-
-
-
-
8
-
7
-
-
1
-
-
-
-
-
10
-
2
1
-
-
-
1
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
1
-
-
-
-
10
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
754410
Zhong
Cloning and characterization ...
Levilactobacillus brevis, Levilactobacillus brevis KB290
J. Microbiol. Biotechnol.
26
1661-1667
2016
-
1
1
-
-
-
-
-
-
-
-
4
-
2
-
-
-
-
-
-
-
-
10
-
4
1
1
3
-
1
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
10
-
1
1
3
-
1
1
-
-
-
1
1
-
-
-
754568
Fu
Biotransformation of ginsenos ...
Arthrinium sp. GE 17-18
Lett. Appl. Microbiol.
63
196-201
2016
-
-
-
-
-
-
-
-
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-
1
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-
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-
3
-
-
-
-
-
-
-
-
-
-
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3
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752583
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An amino acid at position 512 ...
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4
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4
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1
1
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1
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4
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4
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1
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1
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1
1
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755274
Liu
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Preparation of minor ginsenos ...
Aspergillus niger, Aspergillus niger g.848
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6
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17
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3
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1
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21
1
1
1
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1
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6
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17
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1
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21
1
1
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1
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722033
Yu
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2
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2
1
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4
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1
1
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1
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11
1
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2
1
1
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1
1
1
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2
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2
1
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1
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1
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11
1
2
1
1
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1
1
1
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