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Literature summary for 3.2.1.192 extracted from

  • Ku, S.; You, H.J.; Park, M.S.; Ji, G.E.
    Whole-cell biocatalysis for producing ginsenoside Rd from Rb1 using Lactobacillus rhamnosus GG (2016), J. Microbiol. Biotechnol., 26, 1206-1215 .
    View publication on PubMed
Search for more literature for 3.2.1.192

Application

Application Comment Organism
synthesis industrial applications of Lactobacillus rhamnosus strain GG to the biocatalysis of ginsenosides and/or other phytochemicals Lacticaseibacillus rhamnosus

General Stability

General Stability Organism
whole-cell homogenates show the highest beta-glucosidase activity when compared with disrupted cell suspensions. The cell disruption step significantly decreases the beta-glucosidase activity Lacticaseibacillus rhamnosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ginsenoside Rb1 + H2O Lacticaseibacillus rhamnosus
-
 ginsenoside Rd + D-glucopyranose
-
 ?
ginsenoside Rb1 + H2O Lacticaseibacillus rhamnosus GG
-
 ginsenoside Rd + D-glucopyranose
-
 ?

Organism

Organism UniProt Comment Textmining
Lacticaseibacillus rhamnosus
-
-
-
Lacticaseibacillus rhamnosus GG
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information at pH 6.0, 40°C, and 2% w/v cellobiose beta-glucosidase production in Lactobacillus rhamnosus strain GG is enhanced by 25fold, optimization of microbial media composition for production of beta-glucosidase from strain GG, overview Lacticaseibacillus rhamnosus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Lacticaseibacillus rhamnosus 4-nitrophenol + beta-D-glucopyranose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Lacticaseibacillus rhamnosus GG 4-nitrophenol + beta-D-glucopyranose
-
 ?
ginsenoside Rb1 + H2O
-
 Lacticaseibacillus rhamnosus ginsenoside Rd + D-glucopyranose
-
 ?
ginsenoside Rb1 + H2O
-
 Lacticaseibacillus rhamnosus GG ginsenoside Rd + D-glucopyranose
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 whole cell Lacticaseibacillus rhamnosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 cell lysate and whole cell Lacticaseibacillus rhamnosus

Expression

Organism Comment Expression
Lacticaseibacillus rhamnosus 2-5% cellobiose induce enzyme expression up