BRENDA - Enzyme Database
show all sequences of 3.2.1.192

Overexpression and characterization of a Ca2+ activated thermostable beta-glucosidase with high ginsenoside Rb1 to ginsenoside 20(S)-Rg3 bioconversion productivity

Xie, J.; Zhao, D.; Zhao, L.; Pei, J.; Xiao, W.; Ding, G.; Wang, Z.; J. Ind. Microbiol. Biotechnol. 42, 839-850 (2015)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
methanol
activates 14% at 5%, inhibits 20% at 30%
Thermotoga petrophila
Cloned(Commentary)
Cloned (Commentary)
Organism
gene Tpet_0898, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) partly in inclusion bodies, method optimization
Thermotoga petrophila
General Stability
General Stability
Organism
Ca2+ and Mn2+ stabilize the purified recombinant enzyme
Thermotoga petrophila
Inhibitors
Inhibitors
Commentary
Organism
Structure
Al3+
67% inhibition at 5 mM
Thermotoga petrophila
Cu2+
40% inhibition at 5 mM
Thermotoga petrophila
DMSO
inhibits 26% at 15% and 42% at 30%
Thermotoga petrophila
ethanol
inhibits 20% at 15% and 45% at 30%
Thermotoga petrophila
Hg2+
complete inhibition at 1-5 mM
Thermotoga petrophila
methanol
activates 14% at 5%, inhibits 20% at 30%
Thermotoga petrophila
additional information
no significant effect of 5 mM EDTA on the enzyme activity
Thermotoga petrophila
NH4+
45% inhibition at 5 mM
Thermotoga petrophila
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
activates about 1.5fold at 5 mM, stabilizes the purified recombinant enzyme
Thermotoga petrophila
Co2+
activates about 1.5fold at 5 mM, does not stabilize the purified recombinant enzyme
Thermotoga petrophila
Mn2+
activates about 1.5fold at 5 mM, stabilizes the purified recombinant enzyme
Thermotoga petrophila
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ginsenoside Rb1 + H2O
Thermotoga petrophila
-
ginsenoside Rd + D-glucopyranose
-
-
?
ginsenoside Rb1 + H2O
Thermotoga petrophila
direct conversion of Rb1 into Rg3
ginsenoside Rg3 + D-glucopyranose
-
-
?
ginsenoside Rb1 + H2O
Thermotoga petrophila DSM 13995
-
ginsenoside Rd + D-glucopyranose
-
-
?
ginsenoside Rb1 + H2O
Thermotoga petrophila RKU-1
-
ginsenoside Rd + D-glucopyranose
-
-
?
ginsenoside Rb1 + H2O
Thermotoga petrophila ATCC BAA-488
-
ginsenoside Rd + D-glucopyranose
-
-
?
ginsenoside Rd + H2O
Thermotoga petrophila
-
ginsenoside Rg3 + D-glucopyranose
-
-
?
ginsenoside Rd + H2O
Thermotoga petrophila DSM 13995
-
ginsenoside Rg3 + D-glucopyranose
-
-
?
ginsenoside Rd + H2O
Thermotoga petrophila RKU-1
-
ginsenoside Rg3 + D-glucopyranose
-
-
?
ginsenoside Rd + H2O
Thermotoga petrophila ATCC BAA-488
-
ginsenoside Rg3 + D-glucopyranose
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Thermotoga petrophila
A5IL43
-
-
Thermotoga petrophila ATCC BAA-488
A5IL43
-
-
Thermotoga petrophila DSM 13995
A5IL43
-
-
Thermotoga petrophila RKU-1
A5IL43
-
-
Purification (Commentary)
Purification (Commentary)
Organism
recombinant His-tagged enzyme 6.1fold from Escherichia coli strain BL21(DE3) by heat treatment and nickel affinity chromatography
Thermotoga petrophila
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
in a reaction at 90°C and pH 5.0, 10 g/l of ginsenoside Rb1 is transformed into 6.93 g/l of Rg3 within 90 min, with a corresponding molar conversion of 97.9 %, and Rg3 productivity of 4620 mg/l/h
Thermotoga petrophila
95.7
-
purified recombinant His-tagged enzyme, pH 5.0, 90°C, substrate is 4-nitrophenyl beta-D-glucopyranoside
Thermotoga petrophila
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2-nitrophenyl beta-D-glucopyranoside + H2O
low activity
754332
Thermotoga petrophila
2-nitrophenol + beta-D-glucopyranose
-
-
-
?
2-nitrophenyl beta-D-glucopyranoside + H2O
low activity
754332
Thermotoga petrophila DSM 13995
2-nitrophenol + beta-D-glucopyranose
-
-
-
?
2-nitrophenyl beta-D-glucopyranoside + H2O
low activity
754332
Thermotoga petrophila RKU-1
2-nitrophenol + beta-D-glucopyranose
-
-
-
?
2-nitrophenyl beta-D-glucopyranoside + H2O
low activity
754332
Thermotoga petrophila ATCC BAA-488
2-nitrophenol + beta-D-glucopyranose
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
754332
Thermotoga petrophila
4-nitrophenol + beta-D-glucopyranose
-
-
-
?
ginsenoside compound K + H2O
35% activity compared to ginsenoside Rb1
754332
Thermotoga petrophila
20(S)-protopanaxadiol + D-glucopyranose
-
-
-
?
ginsenoside F2 + H2O
91% activity compared to ginsenoside Rb1
754332
Thermotoga petrophila
ginsenoside 20(S)-Rh2 + D-glucopyranose
-
-
-
?
ginsenoside Rb1 + H2O
-
754332
Thermotoga petrophila
ginsenoside Rd + D-glucopyranose
-
-
-
?
ginsenoside Rb1 + H2O
best ginsenoside substrate
754332
Thermotoga petrophila
ginsenoside Rd + D-glucopyranose
-
-
-
?
ginsenoside Rb1 + H2O
-
754332
Thermotoga petrophila DSM 13995
ginsenoside Rd + D-glucopyranose
-
-
-
?
ginsenoside Rb1 + H2O
best ginsenoside substrate
754332
Thermotoga petrophila DSM 13995
ginsenoside Rd + D-glucopyranose
-
-
-
?
ginsenoside Rb1 + H2O
-
754332
Thermotoga petrophila RKU-1
ginsenoside Rd + D-glucopyranose
-
-
-
?
ginsenoside Rb1 + H2O
best ginsenoside substrate
754332
Thermotoga petrophila RKU-1
ginsenoside Rd + D-glucopyranose
-
-
-
?
ginsenoside Rb1 + H2O
-
754332
Thermotoga petrophila ATCC BAA-488
ginsenoside Rd + D-glucopyranose
-
-
-
?
ginsenoside Rb1 + H2O
best ginsenoside substrate
754332
Thermotoga petrophila ATCC BAA-488
ginsenoside Rd + D-glucopyranose
-
-
-
?
ginsenoside Rb1 + H2O
direct conversion of Rb1 into Rg3
754332
Thermotoga petrophila
ginsenoside Rg3 + D-glucopyranose
-
-
-
?
ginsenoside Rd + H2O
-
754332
Thermotoga petrophila
ginsenoside Rg3 + D-glucopyranose
-
-
-
?
ginsenoside Rd + H2O
-
754332
Thermotoga petrophila DSM 13995
ginsenoside Rg3 + D-glucopyranose
-
-
-
?
ginsenoside Rd + H2O
-
754332
Thermotoga petrophila RKU-1
ginsenoside Rg3 + D-glucopyranose
-
-
-
?
ginsenoside Rd + H2O
-
754332
Thermotoga petrophila ATCC BAA-488
ginsenoside Rg3 + D-glucopyranose
-
-
-
?
ginsenoside Rd + H2O
84% activity compared to ginsenoside Rb1
754332
Thermotoga petrophila
ginsenoside 20(S)-Rg3 + D-glucopyranose
-
-
-
?
ginsenoside Re + H2O
81% activity compared to ginsenoside Rb1
754332
Thermotoga petrophila
ginsenoside Rg2 + D-glucopyranose
-
-
-
?
ginsenoside Rg1 + H2O
64% activity compared to ginsenoside Rb1
754332
Thermotoga petrophila
ginsenoside 20(S)-Rh1 + D-glucopyranose
-
-
-
?
additional information
the beta-glucosidase shows preference for cleaving the outer and inner glucopyranosyl moieties at the C-20 carbon of ginsenoside Rb1, which produces the pharmacologically active minor ginsenoside 20(S)-Rg3, via graded hydrolysis of the two glucoses at C-20 or direct hydrolysis of the inner glucoses at C-20. The ginsenoside Rg3 exists as two optical isomers, ginsenoside 20(S)-Rg3 and 20(R)-Rg3. The 20(S)-Rg3 isomer is superior to the 20(R)-Rg3 isomer in terms of its water solubility and bioavailability because of the spatial arrangement of the hydroxyl group on carbon 20. No or poor activity with ginsenosides Rb2, Rc, (S)-Rh2, (S)-Rg3, Rg2, and (S)-Rh1 as substrates, no activity with 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-alpha--L-arabinofuranoside, 4-nitrophenyl-alpha-L-arabinopyranoside, 4-nitrophenyl-alpha-L-rhamnopyranoside, 4-nitrophenyl-beta-D-xylopyranoside. Low activity with gentiobiose and cellobiose. No activity with laminaribiose and sucrose. The enzyme also has broadly specific beta-glucosidase activity against a wide range of substrates with different glycosidic bonds, including aryl-beta-glucosidic bonds and alkyl-beta-glucosidic bonds, and oligosaccharides
754332
Thermotoga petrophila
?
-
-
-
?
additional information
the beta-glucosidase shows preference for cleaving the outer and inner glucopyranosyl moieties at the C-20 carbon of ginsenoside Rb1, which produces the pharmacologically active minor ginsenoside 20(S)-Rg3, via graded hydrolysis of the two glucoses at C-20 or direct hydrolysis of the inner glucoses at C-20. The ginsenoside Rg3 exists as two optical isomers, ginsenoside 20(S)-Rg3 and 20(R)-Rg3. The 20(S)-Rg3 isomer is superior to the 20(R)-Rg3 isomer in terms of its water solubility and bioavailability because of the spatial arrangement of the hydroxyl group on carbon 20. No or poor activity with ginsenosides Rb2, Rc, (S)-Rh2, (S)-Rg3, Rg2, and (S)-Rh1 as substrates, no activity with 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-alpha--L-arabinofuranoside, 4-nitrophenyl-alpha-L-arabinopyranoside, 4-nitrophenyl-alpha-L-rhamnopyranoside, 4-nitrophenyl-beta-D-xylopyranoside. Low activity with gentiobiose and cellobiose. No activity with laminaribiose and sucrose. The enzyme also has broadly specific beta-glucosidase activity against a wide range of substrates with different glycosidic bonds, including aryl-beta-glucosidic bonds and alkyl-beta-glucosidic bonds, and oligosaccharides
754332
Thermotoga petrophila DSM 13995
?
-
-
-
?
additional information
the beta-glucosidase shows preference for cleaving the outer and inner glucopyranosyl moieties at the C-20 carbon of ginsenoside Rb1, which produces the pharmacologically active minor ginsenoside 20(S)-Rg3, via graded hydrolysis of the two glucoses at C-20 or direct hydrolysis of the inner glucoses at C-20. The ginsenoside Rg3 exists as two optical isomers, ginsenoside 20(S)-Rg3 and 20(R)-Rg3. The 20(S)-Rg3 isomer is superior to the 20(R)-Rg3 isomer in terms of its water solubility and bioavailability because of the spatial arrangement of the hydroxyl group on carbon 20. No or poor activity with ginsenosides Rb2, Rc, (S)-Rh2, (S)-Rg3, Rg2, and (S)-Rh1 as substrates, no activity with 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-alpha--L-arabinofuranoside, 4-nitrophenyl-alpha-L-arabinopyranoside, 4-nitrophenyl-alpha-L-rhamnopyranoside, 4-nitrophenyl-beta-D-xylopyranoside. Low activity with gentiobiose and cellobiose. No activity with laminaribiose and sucrose. The enzyme also has broadly specific beta-glucosidase activity against a wide range of substrates with different glycosidic bonds, including aryl-beta-glucosidic bonds and alkyl-beta-glucosidic bonds, and oligosaccharides
754332
Thermotoga petrophila RKU-1
?
-
-
-
?
additional information
the beta-glucosidase shows preference for cleaving the outer and inner glucopyranosyl moieties at the C-20 carbon of ginsenoside Rb1, which produces the pharmacologically active minor ginsenoside 20(S)-Rg3, via graded hydrolysis of the two glucoses at C-20 or direct hydrolysis of the inner glucoses at C-20. The ginsenoside Rg3 exists as two optical isomers, ginsenoside 20(S)-Rg3 and 20(R)-Rg3. The 20(S)-Rg3 isomer is superior to the 20(R)-Rg3 isomer in terms of its water solubility and bioavailability because of the spatial arrangement of the hydroxyl group on carbon 20. No or poor activity with ginsenosides Rb2, Rc, (S)-Rh2, (S)-Rg3, Rg2, and (S)-Rh1 as substrates, no activity with 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-alpha--L-arabinofuranoside, 4-nitrophenyl-alpha-L-arabinopyranoside, 4-nitrophenyl-alpha-L-rhamnopyranoside, 4-nitrophenyl-beta-D-xylopyranoside. Low activity with gentiobiose and cellobiose. No activity with laminaribiose and sucrose. The enzyme also has broadly specific beta-glucosidase activity against a wide range of substrates with different glycosidic bonds, including aryl-beta-glucosidic bonds and alkyl-beta-glucosidic bonds, and oligosaccharides
754332
Thermotoga petrophila ATCC BAA-488
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 81000, recombinant His-tagged enzyme, SDS-PAGE, x * 81243, sequence calculation
Thermotoga petrophila
Synonyms
Synonyms
Commentary
Organism
More
cf. EC 3.2.1.21
Thermotoga petrophila
Tpebgl3
-
Thermotoga petrophila
Tpet_0898
-
Thermotoga petrophila
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
90
-
recombinant enzyme
Thermotoga petrophila
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
75
98
over 50% of maximal activity within this range
Thermotoga petrophila
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
70
-
purified recombinant His-tagged enzyme, pH 5.0, about 90% activity remaining after 180 min
Thermotoga petrophila
80
-
purified recombinant His-tagged enzyme, pH 5.0, about 70% activity remaining after 180 min
Thermotoga petrophila
90
-
purified recombinant His-tagged enzyme, pH 5.0, about 45% activity remaining after 180 min
Thermotoga petrophila
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
recombinant enzyme
Thermotoga petrophila
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4
6
over 50% of maximal activity within this range
Thermotoga petrophila
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
3.5
7.5
purified recombinant His-tagged enzyme, completely stable at pH 7.5, 50% activity at pH 3.5
Thermotoga petrophila
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Thermotoga petrophila
sequence calculation
-
5.28
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
methanol
activates 14% at 5%, inhibits 20% at 30%
Thermotoga petrophila
Cloned(Commentary) (protein specific)
Commentary
Organism
gene Tpet_0898, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) partly in inclusion bodies, method optimization
Thermotoga petrophila
General Stability (protein specific)
General Stability
Organism
Ca2+ and Mn2+ stabilize the purified recombinant enzyme
Thermotoga petrophila
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Al3+
67% inhibition at 5 mM
Thermotoga petrophila
Cu2+
40% inhibition at 5 mM
Thermotoga petrophila
DMSO
inhibits 26% at 15% and 42% at 30%
Thermotoga petrophila
ethanol
inhibits 20% at 15% and 45% at 30%
Thermotoga petrophila
Hg2+
complete inhibition at 1-5 mM
Thermotoga petrophila
methanol
activates 14% at 5%, inhibits 20% at 30%
Thermotoga petrophila
additional information
no significant effect of 5 mM EDTA on the enzyme activity
Thermotoga petrophila
NH4+
45% inhibition at 5 mM
Thermotoga petrophila
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
activates about 1.5fold at 5 mM, stabilizes the purified recombinant enzyme
Thermotoga petrophila
Co2+
activates about 1.5fold at 5 mM, does not stabilize the purified recombinant enzyme
Thermotoga petrophila
Mn2+
activates about 1.5fold at 5 mM, stabilizes the purified recombinant enzyme
Thermotoga petrophila
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ginsenoside Rb1 + H2O
Thermotoga petrophila
-
ginsenoside Rd + D-glucopyranose
-
-
?
ginsenoside Rb1 + H2O
Thermotoga petrophila
direct conversion of Rb1 into Rg3
ginsenoside Rg3 + D-glucopyranose
-
-
?
ginsenoside Rb1 + H2O
Thermotoga petrophila DSM 13995
-
ginsenoside Rd + D-glucopyranose
-
-
?
ginsenoside Rb1 + H2O
Thermotoga petrophila RKU-1
-
ginsenoside Rd + D-glucopyranose
-
-
?
ginsenoside Rb1 + H2O
Thermotoga petrophila ATCC BAA-488
-
ginsenoside Rd + D-glucopyranose
-
-
?
ginsenoside Rd + H2O
Thermotoga petrophila
-
ginsenoside Rg3 + D-glucopyranose
-
-
?
ginsenoside Rd + H2O
Thermotoga petrophila DSM 13995
-
ginsenoside Rg3 + D-glucopyranose
-
-
?
ginsenoside Rd + H2O
Thermotoga petrophila RKU-1
-
ginsenoside Rg3 + D-glucopyranose
-
-
?
ginsenoside Rd + H2O
Thermotoga petrophila ATCC BAA-488
-
ginsenoside Rg3 + D-glucopyranose
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged enzyme 6.1fold from Escherichia coli strain BL21(DE3) by heat treatment and nickel affinity chromatography
Thermotoga petrophila
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
in a reaction at 90°C and pH 5.0, 10 g/l of ginsenoside Rb1 is transformed into 6.93 g/l of Rg3 within 90 min, with a corresponding molar conversion of 97.9 %, and Rg3 productivity of 4620 mg/l/h
Thermotoga petrophila
95.7
-
purified recombinant His-tagged enzyme, pH 5.0, 90°C, substrate is 4-nitrophenyl beta-D-glucopyranoside
Thermotoga petrophila
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2-nitrophenyl beta-D-glucopyranoside + H2O
low activity
754332
Thermotoga petrophila
2-nitrophenol + beta-D-glucopyranose
-
-
-
?
2-nitrophenyl beta-D-glucopyranoside + H2O
low activity
754332
Thermotoga petrophila DSM 13995
2-nitrophenol + beta-D-glucopyranose
-
-
-
?
2-nitrophenyl beta-D-glucopyranoside + H2O
low activity
754332
Thermotoga petrophila RKU-1
2-nitrophenol + beta-D-glucopyranose
-
-
-
?
2-nitrophenyl beta-D-glucopyranoside + H2O
low activity
754332
Thermotoga petrophila ATCC BAA-488
2-nitrophenol + beta-D-glucopyranose
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
754332
Thermotoga petrophila
4-nitrophenol + beta-D-glucopyranose
-
-
-
?
ginsenoside compound K + H2O
35% activity compared to ginsenoside Rb1
754332
Thermotoga petrophila
20(S)-protopanaxadiol + D-glucopyranose
-
-
-
?
ginsenoside F2 + H2O
91% activity compared to ginsenoside Rb1
754332
Thermotoga petrophila
ginsenoside 20(S)-Rh2 + D-glucopyranose
-
-
-
?
ginsenoside Rb1 + H2O
-
754332
Thermotoga petrophila
ginsenoside Rd + D-glucopyranose
-
-
-
?
ginsenoside Rb1 + H2O
best ginsenoside substrate
754332
Thermotoga petrophila
ginsenoside Rd + D-glucopyranose
-
-
-
?
ginsenoside Rb1 + H2O
-
754332
Thermotoga petrophila DSM 13995
ginsenoside Rd + D-glucopyranose
-
-
-
?
ginsenoside Rb1 + H2O
best ginsenoside substrate
754332
Thermotoga petrophila DSM 13995
ginsenoside Rd + D-glucopyranose
-
-
-
?
ginsenoside Rb1 + H2O
-
754332
Thermotoga petrophila RKU-1
ginsenoside Rd + D-glucopyranose
-
-
-
?
ginsenoside Rb1 + H2O
best ginsenoside substrate
754332
Thermotoga petrophila RKU-1
ginsenoside Rd + D-glucopyranose
-
-
-
?
ginsenoside Rb1 + H2O
-
754332
Thermotoga petrophila ATCC BAA-488
ginsenoside Rd + D-glucopyranose
-
-
-
?
ginsenoside Rb1 + H2O
best ginsenoside substrate
754332
Thermotoga petrophila ATCC BAA-488
ginsenoside Rd + D-glucopyranose
-
-
-
?
ginsenoside Rb1 + H2O
direct conversion of Rb1 into Rg3
754332
Thermotoga petrophila
ginsenoside Rg3 + D-glucopyranose
-
-
-
?
ginsenoside Rd + H2O
-
754332
Thermotoga petrophila
ginsenoside Rg3 + D-glucopyranose
-
-
-
?
ginsenoside Rd + H2O
-
754332
Thermotoga petrophila DSM 13995
ginsenoside Rg3 + D-glucopyranose
-
-
-
?
ginsenoside Rd + H2O
-
754332
Thermotoga petrophila RKU-1
ginsenoside Rg3 + D-glucopyranose
-
-
-
?
ginsenoside Rd + H2O
-
754332
Thermotoga petrophila ATCC BAA-488
ginsenoside Rg3 + D-glucopyranose
-
-
-
?
ginsenoside Rd + H2O
84% activity compared to ginsenoside Rb1
754332
Thermotoga petrophila
ginsenoside 20(S)-Rg3 + D-glucopyranose
-
-
-
?
ginsenoside Re + H2O
81% activity compared to ginsenoside Rb1
754332
Thermotoga petrophila
ginsenoside Rg2 + D-glucopyranose
-
-
-
?
ginsenoside Rg1 + H2O
64% activity compared to ginsenoside Rb1
754332
Thermotoga petrophila
ginsenoside 20(S)-Rh1 + D-glucopyranose
-
-
-
?
additional information
the beta-glucosidase shows preference for cleaving the outer and inner glucopyranosyl moieties at the C-20 carbon of ginsenoside Rb1, which produces the pharmacologically active minor ginsenoside 20(S)-Rg3, via graded hydrolysis of the two glucoses at C-20 or direct hydrolysis of the inner glucoses at C-20. The ginsenoside Rg3 exists as two optical isomers, ginsenoside 20(S)-Rg3 and 20(R)-Rg3. The 20(S)-Rg3 isomer is superior to the 20(R)-Rg3 isomer in terms of its water solubility and bioavailability because of the spatial arrangement of the hydroxyl group on carbon 20. No or poor activity with ginsenosides Rb2, Rc, (S)-Rh2, (S)-Rg3, Rg2, and (S)-Rh1 as substrates, no activity with 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-alpha--L-arabinofuranoside, 4-nitrophenyl-alpha-L-arabinopyranoside, 4-nitrophenyl-alpha-L-rhamnopyranoside, 4-nitrophenyl-beta-D-xylopyranoside. Low activity with gentiobiose and cellobiose. No activity with laminaribiose and sucrose. The enzyme also has broadly specific beta-glucosidase activity against a wide range of substrates with different glycosidic bonds, including aryl-beta-glucosidic bonds and alkyl-beta-glucosidic bonds, and oligosaccharides
754332
Thermotoga petrophila
?
-
-
-
?
additional information
the beta-glucosidase shows preference for cleaving the outer and inner glucopyranosyl moieties at the C-20 carbon of ginsenoside Rb1, which produces the pharmacologically active minor ginsenoside 20(S)-Rg3, via graded hydrolysis of the two glucoses at C-20 or direct hydrolysis of the inner glucoses at C-20. The ginsenoside Rg3 exists as two optical isomers, ginsenoside 20(S)-Rg3 and 20(R)-Rg3. The 20(S)-Rg3 isomer is superior to the 20(R)-Rg3 isomer in terms of its water solubility and bioavailability because of the spatial arrangement of the hydroxyl group on carbon 20. No or poor activity with ginsenosides Rb2, Rc, (S)-Rh2, (S)-Rg3, Rg2, and (S)-Rh1 as substrates, no activity with 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-alpha--L-arabinofuranoside, 4-nitrophenyl-alpha-L-arabinopyranoside, 4-nitrophenyl-alpha-L-rhamnopyranoside, 4-nitrophenyl-beta-D-xylopyranoside. Low activity with gentiobiose and cellobiose. No activity with laminaribiose and sucrose. The enzyme also has broadly specific beta-glucosidase activity against a wide range of substrates with different glycosidic bonds, including aryl-beta-glucosidic bonds and alkyl-beta-glucosidic bonds, and oligosaccharides
754332
Thermotoga petrophila DSM 13995
?
-
-
-
?
additional information
the beta-glucosidase shows preference for cleaving the outer and inner glucopyranosyl moieties at the C-20 carbon of ginsenoside Rb1, which produces the pharmacologically active minor ginsenoside 20(S)-Rg3, via graded hydrolysis of the two glucoses at C-20 or direct hydrolysis of the inner glucoses at C-20. The ginsenoside Rg3 exists as two optical isomers, ginsenoside 20(S)-Rg3 and 20(R)-Rg3. The 20(S)-Rg3 isomer is superior to the 20(R)-Rg3 isomer in terms of its water solubility and bioavailability because of the spatial arrangement of the hydroxyl group on carbon 20. No or poor activity with ginsenosides Rb2, Rc, (S)-Rh2, (S)-Rg3, Rg2, and (S)-Rh1 as substrates, no activity with 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-alpha--L-arabinofuranoside, 4-nitrophenyl-alpha-L-arabinopyranoside, 4-nitrophenyl-alpha-L-rhamnopyranoside, 4-nitrophenyl-beta-D-xylopyranoside. Low activity with gentiobiose and cellobiose. No activity with laminaribiose and sucrose. The enzyme also has broadly specific beta-glucosidase activity against a wide range of substrates with different glycosidic bonds, including aryl-beta-glucosidic bonds and alkyl-beta-glucosidic bonds, and oligosaccharides
754332
Thermotoga petrophila RKU-1
?
-
-
-
?
additional information
the beta-glucosidase shows preference for cleaving the outer and inner glucopyranosyl moieties at the C-20 carbon of ginsenoside Rb1, which produces the pharmacologically active minor ginsenoside 20(S)-Rg3, via graded hydrolysis of the two glucoses at C-20 or direct hydrolysis of the inner glucoses at C-20. The ginsenoside Rg3 exists as two optical isomers, ginsenoside 20(S)-Rg3 and 20(R)-Rg3. The 20(S)-Rg3 isomer is superior to the 20(R)-Rg3 isomer in terms of its water solubility and bioavailability because of the spatial arrangement of the hydroxyl group on carbon 20. No or poor activity with ginsenosides Rb2, Rc, (S)-Rh2, (S)-Rg3, Rg2, and (S)-Rh1 as substrates, no activity with 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-alpha--L-arabinofuranoside, 4-nitrophenyl-alpha-L-arabinopyranoside, 4-nitrophenyl-alpha-L-rhamnopyranoside, 4-nitrophenyl-beta-D-xylopyranoside. Low activity with gentiobiose and cellobiose. No activity with laminaribiose and sucrose. The enzyme also has broadly specific beta-glucosidase activity against a wide range of substrates with different glycosidic bonds, including aryl-beta-glucosidic bonds and alkyl-beta-glucosidic bonds, and oligosaccharides
754332
Thermotoga petrophila ATCC BAA-488
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 81000, recombinant His-tagged enzyme, SDS-PAGE, x * 81243, sequence calculation
Thermotoga petrophila
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
90
-
recombinant enzyme
Thermotoga petrophila
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
75
98
over 50% of maximal activity within this range
Thermotoga petrophila
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
70
-
purified recombinant His-tagged enzyme, pH 5.0, about 90% activity remaining after 180 min
Thermotoga petrophila
80
-
purified recombinant His-tagged enzyme, pH 5.0, about 70% activity remaining after 180 min
Thermotoga petrophila
90
-
purified recombinant His-tagged enzyme, pH 5.0, about 45% activity remaining after 180 min
Thermotoga petrophila
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
recombinant enzyme
Thermotoga petrophila
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4
6
over 50% of maximal activity within this range
Thermotoga petrophila
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
3.5
7.5
purified recombinant His-tagged enzyme, completely stable at pH 7.5, 50% activity at pH 3.5
Thermotoga petrophila
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Thermotoga petrophila
sequence calculation
-
5.28
General Information
General Information
Commentary
Organism
evolution
the enzyme belongs to the glycosyl hydrolase family 3, GH3. Alignment of the Tpebgl3 cluster with several GH3 beta-glucosidases indicates that they share some conserved motifs: CIHKFV (residues 162-167), GFVMSDWYAGDN (residues 238-249) and IVISRISGEGYDRK (residues 452-463)
Thermotoga petrophila
General Information (protein specific)
General Information
Commentary
Organism
evolution
the enzyme belongs to the glycosyl hydrolase family 3, GH3. Alignment of the Tpebgl3 cluster with several GH3 beta-glucosidases indicates that they share some conserved motifs: CIHKFV (residues 162-167), GFVMSDWYAGDN (residues 238-249) and IVISRISGEGYDRK (residues 452-463)
Thermotoga petrophila
Other publictions for EC 3.2.1.192
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
753988
Fu
Biotransformation of ginsenos ...
Burkholderia sp. GE 17-7
J. Appl. Microbiol.
122
1579-1585
2017
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
3
-
-
1
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
3
-
1
1
-
-
1
1
-
-
-
-
-
-
-
-
754566
Renchinkhand
Characterization of Paenibaci ...
Paenibacillus sp. MBT213
Korean J. Food Sci. Anim. Resour.
37
735-742
2017
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
1
-
1
-
-
1
-
-
-
1
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
1
-
-
-
1
1
-
-
-
1
1
-
-
-
753042
Huq
-
Enzymatic transformation of g ...
Microbacterium esteraromaticum
Biotechnol. Appl. Biochem.
63
532-538
2016
-
-
1
-
-
-
-
-
-
-
-
5
-
1
-
-
-
-
-
-
-
-
6
-
4
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
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5
-
-
-
-
-
-
-
-
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
754409
Ku
Whole-cell biocatalysis for p ...
Lacticaseibacillus rhamnosus, Lacticaseibacillus rhamnosus GG
J. Microbiol. Biotechnol.
26
1206-1215
2016
-
1
-
-
-
1
-
-
-
-
-
2
-
4
-
-
-
-
-
1
-
-
4
-
-
1
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
4
-
1
-
-
-
1
-
-
-
1
-
-
1
-
-
752518
Lin
Efficient biotransformation o ...
Aspergillus versicolor, Aspergillus versicolor LFJ1403
Antonie van Leeuwenhoek
108
1117-1127
2015
-
-
-
-
-
-
-
-
1
-
-
2
-
2
-
-
-
-
-
1
-
-
4
-
1
1
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
4
-
1
1
-
-
1
-
-
-
-
-
-
-
-
-
754213
Shin
Substrate specificity of beta ...
Gordonia terrae, Gordonia terrae DSM 43249, Gordonia terrae NBRC 100016
J. Biosci. Bioeng.
119
497-504
2015
-
-
1
-
-
-
-
4
-
-
1
2
-
3
-
-
1
-
-
-
3
-
5
-
1
1
1
-
3
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
4
-
-
1
2
-
-
-
1
-
-
3
-
5
-
1
1
-
3
1
1
-
-
-
-
-
-
3
3
754332
Xie
Overexpression and characteri ...
Thermotoga petrophila, Thermotoga petrophila DSM 13995, Thermotoga petrophila RKU-1, Thermotoga petrophila ATCC BAA-488
J. Ind. Microbiol. Biotechnol.
42
839-850
2015
1
-
1
-
-
1
8
-
-
3
-
9
-
6
-
-
1
-
-
-
2
-
27
1
3
1
1
3
-
1
1
1
-
-
1
-
1
-
1
-
-
-
1
-
8
-
-
-
3
-
9
-
-
-
1
-
-
2
-
27
1
1
1
3
-
1
1
1
1
-
1
1
-
-
-
754434
Pei
-
Enzymatic transformation of g ...
Pseudothermotoga thermarum, Pseudothermotoga thermarum DSM 5069 T
J. Mol. Catal. B
113
104-109
2015
3
-
1
-
-
-
7
1
-
1
-
5
-
2
-
-
1
-
-
-
2
-
16
1
6
1
1
3
1
1
1
2
-
-
-
-
3
-
1
-
-
-
-
-
7
-
1
-
1
-
5
-
-
-
1
-
-
2
-
16
1
1
1
3
1
1
1
2
-
-
1
1
-
1
1
755376
Wan
-
Highly efficient biotransform ...
Aspergillus sp.
RSC Adv.
5
78874-78879
2015
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
5
-
1
1
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
5
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
678825
Yan
Purification method improvemen ...
Paecilomyces sp. (in: Eurotiomycetes), Paecilomyces sp. (in: Eurotiomycetes) 220 Bainier
Biosci. Biotechnol. Biochem.
72
352-359
2008
-
-
-
-
-
-
-
3
-
-
2
-
-
2
-
-
1
1
-
-
-
-
5
1
-
2
1
2
-
1
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
2
-
-
-
-
1
-
-
-
-
5
1
2
1
2
-
1
1
2
-
-
-
-
-
-
-