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Literature summary for 3.2.1.192 extracted from

  • Yan, Q.; Zhou, W.; Li, X.; Feng, M.; Zhou, P.
    Purification method improvement and characterization of a novel ginsenoside-hydrolyzing beta-glucosidase from Paecilomyces Bainier sp. 229 (2008), Biosci. Biotechnol. Biochem., 72, 352-359.
    View publication on PubMed
Search for more literature for 3.2.1.192

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.072
-
 ginsenoside Rb1 pH 3.5, 45°C Paecilomyces sp. (in: Eurotiomycetes)
0.111
-
 4-nitrophenyl beta-D-glucoside pH 3.5, 45°C Paecilomyces sp. (in: Eurotiomycetes)
0.805
-
 ginsenoside Rd pH 3.5, 45°C Paecilomyces sp. (in: Eurotiomycetes)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
102000
-
 3 * 102000, SDS-PAGE Paecilomyces sp. (in: Eurotiomycetes)
305000
-
 gel filtration Paecilomyces sp. (in: Eurotiomycetes)

Organism

Organism UniProt Comment Textmining
Paecilomyces sp. (in: Eurotiomycetes)
-
-
-
Paecilomyces sp. (in: Eurotiomycetes) 220 Bainier
-
-
-

Purification (Commentary)

Purification (Comment) Organism
only two chromatographic steps, QSepharose FF and microcrystalline cellulose column in sequence, are required to purify the enzyme to homogeneity Paecilomyces sp. (in: Eurotiomycetes)

Reaction

Reaction Comment Organism Reaction ID
ginsenoside Rb1 + 2 H2O = ginsenoside Rg3 + 2 D-glucopyranose overall reaction Paecilomyces sp. (in: Eurotiomycetes)
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-glucoside + H2O no hydrolysis of 4-nitrophenyl-alpha-D-glucoside or 4-nitrophenyl-beta-D-xyloside Paecilomyces sp. (in: Eurotiomycetes) 4-nitrophenol + D-glucopyranose
-
 ?
gentiobiose + H2O
-
 Paecilomyces sp. (in: Eurotiomycetes) D-glucopyranose
-
 ?
ginsenoside Rb1 + H2O hydrolysis of 20-O-beta-D-glucosyl-beta-D-(1->6)-glucosidic bond Paecilomyces sp. (in: Eurotiomycetes) ginsenoside Rd + D-glucopyranose
-
 ?
ginsenoside Rd + H2O hydrolyzes the 20-O-glucoside of Rd at 44% of the activity compared to the hydrolysis of the 20-O-beta-D-glucosyl-beta-D-(1->6)-glucosidic bond of ginsenoside Rb1 Paecilomyces sp. (in: Eurotiomycetes) ginsenoside Rg3 + D-glucopyranose
-
 ?
additional information no hydrolysis of the 20-O-alpha-D-(1->6)-arabinopyranosidic bond of ginsenoside Rb2 and the 20-O-beta-D-(1->6)-xylosidic bond of ginsenoside Rb3. It does not hydrolyse the 3-O-beta-glucosidic bond of ginsenoside Rb1, Rb2, Rb3 Paecilomyces sp. (in: Eurotiomycetes) ?
-
 ?

Subunits

Subunits Comment Organism
trimer 3 * 102000, SDS-PAGE Paecilomyces sp. (in: Eurotiomycetes)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 assay at Paecilomyces sp. (in: Eurotiomycetes)
55
-
-
 Paecilomyces sp. (in: Eurotiomycetes)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 60 40°C: about 50% of maximal activity. 60°C: about 60% of maximal activity Paecilomyces sp. (in: Eurotiomycetes)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 pH 3.5, 4 h, 10.4% loss of activity Paecilomyces sp. (in: Eurotiomycetes)
55
-
 pH 3.5, 4 h, 81.5% loss of activity Paecilomyces sp. (in: Eurotiomycetes)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5
-
 at 55°C, in 50 mM formate buffer Paecilomyces sp. (in: Eurotiomycetes)

pH Range

pH Minimum pH Maximum Comment Organism
2.5 5 pH 2.5: about 45% of maximal activity, pH 5.0: about 45% of maximal activity Paecilomyces sp. (in: Eurotiomycetes)

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 7 45°C, 4 h, 10% loss of activity Paecilomyces sp. (in: Eurotiomycetes)
3.5 5 45°C, 4 h, 5% loss of activity Paecilomyces sp. (in: Eurotiomycetes)