Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.191 extracted from

  • Wan, H.; Li, D.
    Highly efficient biotransformation of ginsenoside Rb1 and Rg3 using beta-galactosidase from Aspergillus sp. (2015), RSC Adv., 5, 78874-78879 .
No PubMed abstract available
Search for more literature for 3.2.1.191

Application

Application Comment Organism
synthesis the beta-galactosidase from Aspergillus sp. can be useful for the mass production of rare ginsenosides Aspergillus sp.

Organism

Organism UniProt Comment Textmining
Aspergillus sp.
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.014
-
 substrate 4-nitrophenyl beta-D-glucopyranoside, pH 5.6, 37°C Aspergillus sp.
6.54
-
 substrate 2-nitrophenyl beta-D-glucopyranoside, pH 5.6, 37°C Aspergillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-glucopyranoside + H2O
-
 Aspergillus sp. 2-nitrophenol + beta-D-glucose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Aspergillus sp. 4-nitrophenol + beta-D-glucose
-
 ?
ginsenoside Rb1 + H2O cf. EC 3.2.1.192 Aspergillus sp. ginsenoside Rd + D-glucopyranose
-
 ?
ginsenoside Rd + H2O
-
 Aspergillus sp. ginsenoside F2 + D-glucopyranose
-
 ?
ginsenoside Rg3 + H2O
-
 Aspergillus sp. ginsenoside Rh2 + D-glucopyranose
-
 ?
additional information beta-galactosidase from Aspergillus sp. displays beta-glucosidase activity, which is responsible for its ability to transform major ginsenoside Rb1 to rare ginsenoside F2 and to ginsenoside Rg3 via ginsenoside Rd. Ginsenoside Rg3 can be selectively hydrolyzed and only Rh2 is obtained with this beta-galactosidase. Before hydrolysis, an Rg3 inclusion complex is prepared with hydroxypropyl-b-cyclodextrin (HP-beta-CD) to improve the aqueous solubility. The solubility of Rg3 increases 74.6fold, and the phase solubility curve displays a typical AL-type, which indicates the formation of a 1:1 inclusion complex. Using an enzyme loading of 500 U/g Rg3, the highest Rg3 conversion of 90.6% and Rh2 yield of 88.5% are obtained after 24 h at 60°C. Ginsenoside F2 is not converted into ginsenoside Rh2 and this is not converted into the protopanaxadiol aglycone Aspergillus sp. ?
-
 ?

Synonyms

Synonyms Comment Organism
More cf. EC 3.2.1.192 Aspergillus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Aspergillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6
-
 assay at Aspergillus sp.