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Literature summary for 3.2.1.191 extracted from
- Classification of glycosidases that hydrolyze the specific positions and types of sugar moieties in ginsenosides (2016), Crit. Rev. Biotechnol., 36, 1036-1049 .
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ginsenoside Rb1 + H2O | Sphingomonas sp. | - |
ginsenoside G17 + D-glucose | - |
? |  |
| ginsenoside Rb1 + H2O | Sphingopyxis alaskensis | - |
ginsenoside G17 + D-glucose | - |
? | |
| ginsenoside Rb1 + H2O | Esteya vermicola | via ginsenoside G17 | ginsenoside G75 + 2 D-glucose | - |
? | |
| ginsenoside Rb1 + H2O | Esteya vermicola CNU120806 | via ginsenoside G17 | ginsenoside G75 + 2 D-glucose | - |
? | |
| ginsenoside Rb2 + H2O | Sphingomonas sp. | - |
ginsenoside C-O + D-glucose | - |
? | |
| ginsenoside Rb2 + H2O | Sphingopyxis alaskensis | - |
ginsenoside C-O + D-glucose | - |
? | |
| ginsenoside Rc + H2O | Sphingomonas sp. | - |
ginsenoside C-Mc1 + D-glucose | - |
? | |
| ginsenoside Rc + H2O | Sphingopyxis alaskensis | - |
ginsenoside C-Mc1 + D-glucose | - |
? | |
| ginsenoside Rd + H2O | Esteya vermicola | via ginsenosside F2 | ginsenoside C-K + 2 D-glucose | - |
? | |
| ginsenoside Rd + H2O | Sphingomonas sp. | - |
ginsenoside F2 + D-glucose | - |
? | |
| ginsenoside Rd + H2O | Sphingopyxis alaskensis | - |
ginsenoside F2 + D-glucose | - |
? | |
| ginsenoside Rd + H2O | Esteya vermicola CNU120806 | via ginsenosside F2 | ginsenoside C-K + 2 D-glucose | - |
? | |
| ginsenoside Rg3 + H2O | Fusarium sp. | - |
ginsenoside Rh2 + D-glucose | - |
? | |
| ginsenoside Rg3 + H2O | Fusarium proliferatum | - |
ginsenoside Rh2 + D-glucose | - |
? | |
| ginsenoside Rg3 + H2O | Panax ginseng | - |
ginsenoside Rh2 + D-glucose | - |
? | |
| ginsenoside Rg3 + H2O | Sphingopyxis alaskensis | - |
ginsenoside Rh2 + D-glucose | - |
? | |
| ginsenoside Rg3 + H2O | Fusarium proliferatum ECU2042 | - |
ginsenoside Rh2 + D-glucose | - |
? | |
| additional information | Aspergillus niger | ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides | ? | - |
? | |
| additional information | Fusarium sp. | ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides | ? | - |
? | |
| additional information | Sphingomonas sp. | ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides | ? | - |
? | |
| additional information | Fusarium proliferatum | ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides | ? | - |
? | |
| additional information | Panax ginseng | ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides | ? | - |
? | |
| additional information | Terrabacter ginsenosidimutans | ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides | ? | - |
? | |
| additional information | Sphingopyxis alaskensis | ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides | ? | - |
? | |
| additional information | Esteya vermicola | ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides | ? | - |
? | |
| additional information | Esteya vermicola CNU120806 | ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides | ? | - |
? | |
| additional information | Fusarium proliferatum ECU2042 | ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
- |
- |
| Esteya vermicola | - |
- |
- |
| Esteya vermicola CNU120806 | - |
- |
- |
| Fusarium proliferatum | - |
- |
- |
| Fusarium proliferatum ECU2042 | - |
- |
- |
| Fusarium sp. | - |
- |
- |
| Panax ginseng | - |
- |
- |
| Sphingomonas sp. | - |
- |
- |
| Sphingopyxis alaskensis | - |
- |
- |
| Terrabacter ginsenosidimutans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme | Esteya vermicola |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2 | - |
pH 5.0, 60°C, substrate ginsenoside Rg3 | Panax ginseng |
| 76 | - |
purified enzyme, pH 7.0, 37°C | Terrabacter ginsenosidimutans |
| 290 | - |
purified native enzyme, pH 5.0, 50°C | Esteya vermicola |
| 309 | - |
pH 5.5, 40°C | Sphingopyxis alaskensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ginsenoside Rb1 + H2O | - |
Sphingomonas sp. | ginsenoside G17 + D-glucose | - |
? | |
| ginsenoside Rb1 + H2O | - |
Sphingopyxis alaskensis | ginsenoside G17 + D-glucose | - |
? | |
| ginsenoside Rb1 + H2O | via ginsenoside G17 | Terrabacter ginsenosidimutans | ginsenoside G75 + 2 D-glucose | - |
? | |
| ginsenoside Rb1 + H2O | via ginsenoside G17 | Esteya vermicola | ginsenoside G75 + 2 D-glucose | - |
? | |
| ginsenoside Rb1 + H2O | via ginsenoside G17 | Esteya vermicola CNU120806 | ginsenoside G75 + 2 D-glucose | - |
? | |
| ginsenoside Rb2 + H2O | - |
Sphingomonas sp. | ginsenoside C-O + D-glucose | - |
? | |
| ginsenoside Rb2 + H2O | - |
Sphingopyxis alaskensis | ginsenoside C-O + D-glucose | - |
? | |
| ginsenoside Rb2 + H2O | via ginsenoside C-O | Terrabacter ginsenosidimutans | ginsenoside C-Y + 2 D-glucose | - |
? | |
| ginsenoside Rb3 + H2O | via ginsenoside C-Mx1 | Terrabacter ginsenosidimutans | ginsenoside C-Mx + 2 D-glucose | - |
? | |
| ginsenoside Rc + H2O | - |
Sphingomonas sp. | ginsenoside C-Mc1 + D-glucose | - |
? | |
| ginsenoside Rc + H2O | - |
Sphingopyxis alaskensis | ginsenoside C-Mc1 + D-glucose | - |
? | |
| ginsenoside Rc + H2O | via ginsenoside C-Mc1 | Terrabacter ginsenosidimutans | ginsenoside C-Mc + 2 D-glucose | - |
? | |
| ginsenoside Rd + H2O | via ginsenoside F2 | Esteya vermicola | ginsenoside C-K + 2 D-glucose | - |
? | |
| ginsenoside Rd + H2O | via ginsenosside F2 | Terrabacter ginsenosidimutans | ginsenoside C-K + 2 D-glucose | - |
? | |
| ginsenoside Rd + H2O | via ginsenosside F2 | Esteya vermicola | ginsenoside C-K + 2 D-glucose | - |
? | |
| ginsenoside Rd + H2O | via ginsenoside F2 | Esteya vermicola CNU120806 | ginsenoside C-K + 2 D-glucose | - |
? | |
| ginsenoside Rd + H2O | via ginsenosside F2 | Esteya vermicola CNU120806 | ginsenoside C-K + 2 D-glucose | - |
? | |
| ginsenoside Rd + H2O | - |
Sphingomonas sp. | ginsenoside F2 + D-glucose | - |
? | |
| ginsenoside Rd + H2O | - |
Sphingopyxis alaskensis | ginsenoside F2 + D-glucose | - |
? | |
| ginsenoside Rg3 + H2O | - |
Fusarium sp. | ginsenoside Rh2 + D-glucose | - |
? | |
| ginsenoside Rg3 + H2O | - |
Fusarium proliferatum | ginsenoside Rh2 + D-glucose | - |
? | |
| ginsenoside Rg3 + H2O | - |
Panax ginseng | ginsenoside Rh2 + D-glucose | - |
? | |
| ginsenoside Rg3 + H2O | - |
Sphingopyxis alaskensis | ginsenoside Rh2 + D-glucose | - |
? | |
| ginsenoside Rg3 + H2O | - |
Fusarium proliferatum ECU2042 | ginsenoside Rh2 + D-glucose | - |
? | |
| ginsenoside Rg3 + H2O | via ginsenoside Rh2 | Terrabacter ginsenosidimutans | protopanaxadiol aglycone + 2 D-glucose | - |
? | |
| ginsenoside Rg3 + H2O | via ginsenoside Rh2, hydrolysis of the inner glucose at C-3 | Aspergillus niger | protopanaxadiol aglycone + 2 D-glucose | - |
? | |
| additional information | ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides | Aspergillus niger | ? | - |
? | |
| additional information | ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides | Fusarium sp. | ? | - |
? | |
| additional information | ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides | Sphingomonas sp. | ? | - |
? | |
| additional information | ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides | Fusarium proliferatum | ? | - |
? | |
| additional information | ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides | Panax ginseng | ? | - |
? | |
| additional information | ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides | Terrabacter ginsenosidimutans | ? | - |
? | |
| additional information | ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides | Sphingopyxis alaskensis | ? | - |
? | |
| additional information | ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides | Esteya vermicola | ? | - |
? | |
| additional information | ginsenosidase type III-i catalyzes the hydrolysis of only C-3 outer glucose in PPD-type gindenosides. Ginsenosidase type III-ii catalyzes the hydrolysis of outer and inner, or only inner glucose in PPD-type ginsenosides | Aspergillus niger | ? | - |
? | |
| additional information | ginsenosidase type III-i catalyzes the hydrolysis of only C-3 outer glucose in PPD-type gindenosides. Ginsenosidase type III-ii catalyzes the hydrolysis of outer and inner, or only inner glucose in PPD-type ginsenosides | Fusarium sp. | ? | - |
? | |
| additional information | ginsenosidase type III-i catalyzes the hydrolysis of only C-3 outer glucose in PPD-type gindenosides. Ginsenosidase type III-ii catalyzes the hydrolysis of outer and inner, or only inner glucose in PPD-type ginsenosides | Sphingomonas sp. | ? | - |
? | |
| additional information | ginsenosidase type III-i catalyzes the hydrolysis of only C-3 outer glucose in PPD-type gindenosides. Ginsenosidase type III-ii catalyzes the hydrolysis of outer and inner, or only inner glucose in PPD-type ginsenosides | Fusarium proliferatum | ? | - |
? | |
| additional information | ginsenosidase type III-i catalyzes the hydrolysis of only C-3 outer glucose in PPD-type gindenosides. Ginsenosidase type III-ii catalyzes the hydrolysis of outer and inner, or only inner glucose in PPD-type ginsenosides | Panax ginseng | ? | - |
? | |
| additional information | ginsenosidase type III-i catalyzes the hydrolysis of only C-3 outer glucose in PPD-type gindenosides. Ginsenosidase type III-ii catalyzes the hydrolysis of outer and inner, or only inner glucose in PPD-type ginsenosides | Terrabacter ginsenosidimutans | ? | - |
? | |
| additional information | ginsenosidase type III-i catalyzes the hydrolysis of only C-3 outer glucose in PPD-type gindenosides. Ginsenosidase type III-ii catalyzes the hydrolysis of outer and inner, or only inner glucose in PPD-type ginsenosides | Esteya vermicola | ? | - |
? | |
| additional information | ginsenosidase type III-i catalyzes the hydrolysis of only C-3 outer glucose in PPD-type gindenosides. Ginsenosidase type III-ii catalyzes the hydrolysis of outer and inner, or only inner glucose in PPD-type ginsenosides. beta-Glucosidase from Sphingopyxis alaskensis hydrolyzes only the outer glucose at C-3 in ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to product G17, C-O, C-Mc1, F2 and Rh2, respectively, without further hydrolysis | Sphingopyxis alaskensis | ? | - |
? | |
| additional information | ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides | Esteya vermicola CNU120806 | ? | - |
? | |
| additional information | ginsenosidase type III-i catalyzes the hydrolysis of only C-3 outer glucose in PPD-type gindenosides. Ginsenosidase type III-ii catalyzes the hydrolysis of outer and inner, or only inner glucose in PPD-type ginsenosides | Esteya vermicola CNU120806 | ? | - |
? | |
| additional information | ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides | Fusarium proliferatum ECU2042 | ? | - |
? | |
| additional information | ginsenosidase type III-i catalyzes the hydrolysis of only C-3 outer glucose in PPD-type gindenosides. Ginsenosidase type III-ii catalyzes the hydrolysis of outer and inner, or only inner glucose in PPD-type ginsenosides | Fusarium proliferatum ECU2042 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 51000 | Sphingopyxis alaskensis |
| ? | x * 49000 | Sphingomonas sp. |
| ? | x * 70000 | Terrabacter ginsenosidimutans |
| ? | x * 59000 | Panax ginseng |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-glucosidase | - |
Sphingomonas sp. |
| beta-glucosidase | - |
Panax ginseng |
| beta-glucosidase | - |
Sphingopyxis alaskensis |
| beta-glucosidase | - |
Esteya vermicola |
| ginsenosidase type III-ii | - |
Aspergillus niger |
| ginsenosidase type III-ii | - |
Fusarium sp. |
| ginsenosidase type III-ii | - |
Sphingomonas sp. |
| ginsenosidase type III-ii | - |
Fusarium proliferatum |
| ginsenosidase type III-ii | - |
Panax ginseng |
| ginsenosidase type III-ii | - |
Terrabacter ginsenosidimutans |
| ginsenosidase type III-ii | - |
Sphingopyxis alaskensis |
| ginsenosidase type III-ii | - |
Esteya vermicola |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Sphingomonas sp. |
| 37 | - |
- |
Terrabacter ginsenosidimutans |
| 40 | - |
- |
Sphingopyxis alaskensis |
| 50 | - |
- |
Esteya vermicola |
| 60 | - |
- |
Panax ginseng |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Sphingomonas sp. |
| 5 | - |
- |
Panax ginseng |
| 5 | - |
- |
Esteya vermicola |
| 5.5 | - |
- |
Sphingopyxis alaskensis |
| 7 | - |
- |
Terrabacter ginsenosidimutans |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the glycosyl hydrolase family 1, GH1 | Sphingomonas sp. |
| evolution | the enzyme belongs to the glycosyl hydrolase family 1, GH1 | Sphingopyxis alaskensis |
| evolution | the enzyme belongs to the glycosyl hydrolase family 3, GH3 | Aspergillus niger |
| evolution | the enzyme belongs to the glycosyl hydrolase family 3, GH3 | Fusarium sp. |
| evolution | the enzyme belongs to the glycosyl hydrolase family 3, GH3 | Terrabacter ginsenosidimutans |
| evolution | the enzyme belongs to the glycosyl hydrolase family 3, GH3 | Esteya vermicola |
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