Kinetics of a cloned special ginsenosidase hydrolyzing 3-O-glucoside of multi-protopanaxadiol-type ginsenosides, named ginsenosidase type III
Jin, X.F.; Yu, H.S.; Wang, D.M.; Liu, T.Q.; Liu, C.Y.; An, D.S.; Im, W.T.; Kim, S.G.; Jin, F.X.; J. Microbiol. Biotechnol. 22, 343-351 (2012) 
Data extracted from this reference:
Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Terrabacter ginsenosidimutans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.85
-
ginsenoside Rc
pH 7.0, 40°C
Terrabacter ginsenosidimutans
4.08
-
ginsenoside Rb1
pH 7.0, 40°C
Terrabacter ginsenosidimutans
8.85
-
ginsenoside Rb3
pH 7.0, 40°C
Terrabacter ginsenosidimutans
9.09
-
ginsenoside Rb2
pH 7.0, 40°C
Terrabacter ginsenosidimutans
9.7
-
ginsenoside Rg3(S)
pH 7.0, 40°C
Terrabacter ginsenosidimutans
11.4
-
ginsenoside Rd
pH 7.0, 40°C
Terrabacter ginsenosidimutans
12.9
-
ginsenoside F2
pH 7.0, 40°C
Terrabacter ginsenosidimutans
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
71140
-
x * 71140, calculated from sequence
Terrabacter ginsenosidimutans
72000
-
x * 72000, SDS-PAGE
Terrabacter ginsenosidimutans
Organism
Organism
UniProt
Commentary
Textmining
Terrabacter ginsenosidimutans
D2KAT1
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Terrabacter ginsenosidimutans
Reaction
Reaction
Commentary
Organism
Reaction ID
a protopanaxadiol-type ginsenoside with two glucosyl residues at position 3 + 2 H2O = a protopanaxadiol-type ginsenoside with no glycosidic modification at position 3 + 2 D-glucopyranose
overall reaction
Terrabacter ginsenosidimutans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
ginsenoside C-Mc1 + H2O
hydrolysis of 3-O-beta-D-glucopyranosyl bond
722936
Terrabacter ginsenosidimutans
ginsenoside C-Mc + D-glucopyranose
-
-
-
?
ginsenoside C-Mx1 + H2O
hydrolysis of 3-O-beta-D-glucopyranosyl bond
722936
Terrabacter ginsenosidimutans
ginsenoside C-Mx + D-glucopyranose
-
-
-
?
ginsenoside C-O + H2O
hydrolysis of 3-O-beta-D-glucopyranosyl bond
722936
Terrabacter ginsenosidimutans
ginsenoside C-Y + D-glucopyranose
-
-
-
?
ginsenoside F2 + H2O
hydrolysis of 3-O-beta-D-glucopyranosyl bond
722936
Terrabacter ginsenosidimutans
ginsenoside C-K + D-glucopyranose
-
-
-
?
ginsenoside Rb1 + H2O
hydrolysis of 3-O-beta-D-(1->2)-glucopyranosyl
722936
Terrabacter ginsenosidimutans
gypenoside XVII + D-glucopyranose
-
-
-
?
ginsenoside Rb2 + H2O
hydrolysis of 3-O-beta-D-(1->2)-glucopyranosyl
722936
Terrabacter ginsenosidimutans
ginsenoside C-O + D-glucopyranose
-
-
-
?
ginsenoside Rb3 + H2O
hydrolysis of 3-O-beta-D-(1->2)-glucopyranosyl bond
722936
Terrabacter ginsenosidimutans
ginsenoside C-Mx1 + D-glucopyranose
-
-
-
?
ginsenoside Rc + H2O
hydrolysis of 3-O-beta-D-(1->2)-glucopyranosyl bond
722936
Terrabacter ginsenosidimutans
ginsenoside C-Mc1 + D-glucopyranose
-
-
-
?
ginsenoside Rd + H2O
hydrolysis of 3-O-beta-D-(1->2)-glucopyranosyl bond
722936
Terrabacter ginsenosidimutans
ginsenoside F2 + D-glucopyranose
-
-
-
?
ginsenoside Rg3(S) + H2O
hydrolysis of 3-O-beta-D-(1->2)-glucopyranosyl bond
722936
Terrabacter ginsenosidimutans
ginsenoside Rh2(S) + D-glucopyranose
-
-
-
?
ginsenoside Rh2(S) + H2O
hydrolysis of 3-O-beta-D-glucopyranosyl bond
722936
Terrabacter ginsenosidimutans
protopanaxadiol aglycone + D-glucopyranose
-
-
-
?
gypenoside XVII + H2O
hydrolysis of 3-O-beta-D-glucopyranosyl
722936
Terrabacter ginsenosidimutans
gypenoside LXXV + D-glucopyranose
-
-
-
?
additional information
the enzyme is able to hydrolyse 3-O-glucoside of multi-protopanaxadiol-type ginsenosides. Similarly, the enzyme can hydrolyse the glucopyranosyls linked to the 3-O-position of Rb2, Rc, Rd, Rb3, and Rg3
722936
Terrabacter ginsenosidimutans
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 72000, SDS-PAGE
Terrabacter ginsenosidimutans
?
x * 71140, calculated from sequence
Terrabacter ginsenosidimutans
Synonyms
Synonyms
Commentary
Organism
3-O-multi-glycoside ginsenosidase
-
Terrabacter ginsenosidimutans
bgpA
-
Terrabacter ginsenosidimutans
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
assay at
Terrabacter ginsenosidimutans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Terrabacter ginsenosidimutans
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Terrabacter ginsenosidimutans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.85
-
ginsenoside Rc
pH 7.0, 40°C
Terrabacter ginsenosidimutans
4.08
-
ginsenoside Rb1
pH 7.0, 40°C
Terrabacter ginsenosidimutans
8.85
-
ginsenoside Rb3
pH 7.0, 40°C
Terrabacter ginsenosidimutans
9.09
-
ginsenoside Rb2
pH 7.0, 40°C
Terrabacter ginsenosidimutans
9.7
-
ginsenoside Rg3(S)
pH 7.0, 40°C
Terrabacter ginsenosidimutans
11.4
-
ginsenoside Rd
pH 7.0, 40°C
Terrabacter ginsenosidimutans
12.9
-
ginsenoside F2
pH 7.0, 40°C
Terrabacter ginsenosidimutans
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
71140
-
x * 71140, calculated from sequence
Terrabacter ginsenosidimutans
72000
-
x * 72000, SDS-PAGE
Terrabacter ginsenosidimutans
Purification (Commentary) (protein specific)
Commentary
Organism
-
Terrabacter ginsenosidimutans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
ginsenoside C-Mc1 + H2O
hydrolysis of 3-O-beta-D-glucopyranosyl bond
722936
Terrabacter ginsenosidimutans
ginsenoside C-Mc + D-glucopyranose
-
-
-
?
ginsenoside C-Mx1 + H2O
hydrolysis of 3-O-beta-D-glucopyranosyl bond
722936
Terrabacter ginsenosidimutans
ginsenoside C-Mx + D-glucopyranose
-
-
-
?
ginsenoside C-O + H2O
hydrolysis of 3-O-beta-D-glucopyranosyl bond
722936
Terrabacter ginsenosidimutans
ginsenoside C-Y + D-glucopyranose
-
-
-
?
ginsenoside F2 + H2O
hydrolysis of 3-O-beta-D-glucopyranosyl bond
722936
Terrabacter ginsenosidimutans
ginsenoside C-K + D-glucopyranose
-
-
-
?
ginsenoside Rb1 + H2O
hydrolysis of 3-O-beta-D-(1->2)-glucopyranosyl
722936
Terrabacter ginsenosidimutans
gypenoside XVII + D-glucopyranose
-
-
-
?
ginsenoside Rb2 + H2O
hydrolysis of 3-O-beta-D-(1->2)-glucopyranosyl
722936
Terrabacter ginsenosidimutans
ginsenoside C-O + D-glucopyranose
-
-
-
?
ginsenoside Rb3 + H2O
hydrolysis of 3-O-beta-D-(1->2)-glucopyranosyl bond
722936
Terrabacter ginsenosidimutans
ginsenoside C-Mx1 + D-glucopyranose
-
-
-
?
ginsenoside Rc + H2O
hydrolysis of 3-O-beta-D-(1->2)-glucopyranosyl bond
722936
Terrabacter ginsenosidimutans
ginsenoside C-Mc1 + D-glucopyranose
-
-
-
?
ginsenoside Rd + H2O
hydrolysis of 3-O-beta-D-(1->2)-glucopyranosyl bond
722936
Terrabacter ginsenosidimutans
ginsenoside F2 + D-glucopyranose
-
-
-
?
ginsenoside Rg3(S) + H2O
hydrolysis of 3-O-beta-D-(1->2)-glucopyranosyl bond
722936
Terrabacter ginsenosidimutans
ginsenoside Rh2(S) + D-glucopyranose
-
-
-
?
ginsenoside Rh2(S) + H2O
hydrolysis of 3-O-beta-D-glucopyranosyl bond
722936
Terrabacter ginsenosidimutans
protopanaxadiol aglycone + D-glucopyranose
-
-
-
?
gypenoside XVII + H2O
hydrolysis of 3-O-beta-D-glucopyranosyl
722936
Terrabacter ginsenosidimutans
gypenoside LXXV + D-glucopyranose
-
-
-
?
additional information
the enzyme is able to hydrolyse 3-O-glucoside of multi-protopanaxadiol-type ginsenosides. Similarly, the enzyme can hydrolyse the glucopyranosyls linked to the 3-O-position of Rb2, Rc, Rd, Rb3, and Rg3
722936
Terrabacter ginsenosidimutans
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 72000, SDS-PAGE
Terrabacter ginsenosidimutans
?
x * 71140, calculated from sequence
Terrabacter ginsenosidimutans
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
assay at
Terrabacter ginsenosidimutans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Terrabacter ginsenosidimutans
Other publictions for EC 3.2.1.191
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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Li
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1
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6
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1
3
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1
1
4
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1
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1
1
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5
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1
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6
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1
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13
1
1
1
3
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1
1
4
1
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3
3
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753334
Shin
Classification of glycosidase ...
Aspergillus niger, Fusarium sp., Sphingomonas sp., Fusarium proliferatum, Panax ginseng, Terrabacter ginsenosidimutans, Sphingopyxis alaskensis, Esteya vermicola, Esteya vermicola CNU120806, Fusarium proliferatum ECU2042
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-
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-
-
-
-
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-
-
-
-
27
-
11
-
-
1
-
-
-
4
-
46
4
12
5
-
-
-
5
-
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-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
27
-
-
-
1
-
-
4
-
46
4
5
-
-
-
5
-
-
-
-
6
6
-
-
-
752583
Shin
An amino acid at position 512 ...
Clavibacter michiganensis, Clavibacter michiganensis DSM 46364
Appl. Microbiol. Biotechnol.
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1
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15
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7
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-
13
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2
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-
-
-
-
24
-
16
-
1
1
-
-
7
1
-
-
-
-
-
-
-
-
1
-
-
15
-
-
-
-
7
-
-
-
13
-
-
-
-
-
-
24
-
16
-
1
-
-
7
1
-
-
-
-
1
1
-
7
7
755376
Wan
-
Highly efficient biotransform ...
Aspergillus sp.
RSC Adv.
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78874-78879
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-
1
-
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-
-
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-
1
-
-
-
-
-
-
2
-
6
-
1
1
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-
1
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-
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-
1
-
-
-
-
-
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-
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-
-
-
-
-
-
-
2
-
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
722936
Jin
Kinetics of a cloned special g ...
Terrabacter ginsenosidimutans
J. Microbiol. Biotechnol.
22
343-351
2012
-
-
1
-
-
-
-
7
-
-
2
-
-
2
-
-
1
1
-
-
-
-
13
2
2
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
7
-
-
2
-
-
-
-
1
-
-
-
-
13
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
713832
An
Identification and characteriz ...
Terrabacter ginsenosidimutans
Appl. Environ. Microbiol.
76
5827-5836
2010
-
-
1
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-
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1
2
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1
3
-
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1
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1
-
-
-
-
-
4
2
1
2
-
2
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1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
2
-
1
3
-
-
-
-
1
-
-
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4
2
2
-
2
-
1
1
-
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-