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Literature summary for 3.2.1.191 extracted from

  • Jin, X.F.; Yu, H.S.; Wang, D.M.; Liu, T.Q.; Liu, C.Y.; An, D.S.; Im, W.T.; Kim, S.G.; Jin, F.X.
    Kinetics of a cloned special ginsenosidase hydrolyzing 3-O-glucoside of multi-protopanaxadiol-type ginsenosides, named ginsenosidase type III (2012), J. Microbiol. Biotechnol., 22, 343-351.
    View publication on PubMed
Search for more literature for 3.2.1.191

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Terrabacter ginsenosidimutans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.85
-
 ginsenoside Rc pH 7.0, 40°C Terrabacter ginsenosidimutans
4.08
-
 ginsenoside Rb1 pH 7.0, 40°C Terrabacter ginsenosidimutans
8.85
-
 ginsenoside Rb3 pH 7.0, 40°C Terrabacter ginsenosidimutans
9.09
-
 ginsenoside Rb2 pH 7.0, 40°C Terrabacter ginsenosidimutans
9.7
-
 ginsenoside Rg3(S) pH 7.0, 40°C Terrabacter ginsenosidimutans
11.4
-
 ginsenoside Rd pH 7.0, 40°C Terrabacter ginsenosidimutans
12.9
-
 ginsenoside F2 pH 7.0, 40°C Terrabacter ginsenosidimutans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
71140
-
 x * 71140, calculated from sequence Terrabacter ginsenosidimutans
72000
-
 x * 72000, SDS-PAGE Terrabacter ginsenosidimutans

Organism

Organism UniProt Comment Textmining
Terrabacter ginsenosidimutans D2KAT1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Terrabacter ginsenosidimutans

Reaction

Reaction Comment Organism Reaction ID
a protopanaxadiol-type ginsenoside with two glucosyl residues at position 3 + 2 H2O = a protopanaxadiol-type ginsenoside with no glycosidic modification at position 3 + 2 D-glucopyranose overall reaction Terrabacter ginsenosidimutans
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ginsenoside C-Mc1 + H2O hydrolysis of 3-O-beta-D-glucopyranosyl bond Terrabacter ginsenosidimutans ginsenoside C-Mc + D-glucopyranose
-
 ?
ginsenoside C-Mx1 + H2O hydrolysis of 3-O-beta-D-glucopyranosyl bond Terrabacter ginsenosidimutans ginsenoside C-Mx + D-glucopyranose
-
 ?
ginsenoside C-O + H2O hydrolysis of 3-O-beta-D-glucopyranosyl bond Terrabacter ginsenosidimutans ginsenoside C-Y + D-glucopyranose
-
 ?
ginsenoside F2 + H2O hydrolysis of 3-O-beta-D-glucopyranosyl bond Terrabacter ginsenosidimutans ginsenoside C-K + D-glucopyranose
-
 ?
ginsenoside Rb1 + H2O hydrolysis of 3-O-beta-D-(1->2)-glucopyranosyl Terrabacter ginsenosidimutans gypenoside XVII + D-glucopyranose
-
 ?
ginsenoside Rb2 + H2O hydrolysis of 3-O-beta-D-(1->2)-glucopyranosyl Terrabacter ginsenosidimutans ginsenoside C-O + D-glucopyranose
-
 ?
ginsenoside Rb3 + H2O hydrolysis of 3-O-beta-D-(1->2)-glucopyranosyl bond Terrabacter ginsenosidimutans ginsenoside C-Mx1 + D-glucopyranose
-
 ?
ginsenoside Rc + H2O hydrolysis of 3-O-beta-D-(1->2)-glucopyranosyl bond Terrabacter ginsenosidimutans ginsenoside C-Mc1 + D-glucopyranose
-
 ?
ginsenoside Rd + H2O hydrolysis of 3-O-beta-D-(1->2)-glucopyranosyl bond Terrabacter ginsenosidimutans ginsenoside F2 + D-glucopyranose
-
 ?
ginsenoside Rg3(S) + H2O hydrolysis of 3-O-beta-D-(1->2)-glucopyranosyl bond Terrabacter ginsenosidimutans ginsenoside Rh2(S) + D-glucopyranose
-
 ?
ginsenoside Rh2(S) + H2O hydrolysis of 3-O-beta-D-glucopyranosyl bond Terrabacter ginsenosidimutans protopanaxadiol aglycone + D-glucopyranose
-
 ?
gypenoside XVII + H2O hydrolysis of 3-O-beta-D-glucopyranosyl Terrabacter ginsenosidimutans gypenoside LXXV + D-glucopyranose
-
 ?
additional information the enzyme is able to hydrolyse 3-O-glucoside of multi-protopanaxadiol-type ginsenosides. Similarly, the enzyme can hydrolyse the glucopyranosyls linked to the 3-O-position of Rb2, Rc, Rd, Rb3, and Rg3 Terrabacter ginsenosidimutans ?
-
 ?

Subunits

Subunits Comment Organism
? x * 72000, SDS-PAGE Terrabacter ginsenosidimutans
? x * 71140, calculated from sequence Terrabacter ginsenosidimutans

Synonyms

Synonyms Comment Organism
3-O-multi-glycoside ginsenosidase
-
 Terrabacter ginsenosidimutans
bgpA
-
 Terrabacter ginsenosidimutans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 assay at Terrabacter ginsenosidimutans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Terrabacter ginsenosidimutans