Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 10 mM, slight inhibition | Absidia sp. (in: Fungi) | |
Cu2+ | 10 mM, significant inhibition | Absidia sp. (in: Fungi) | |
Hg2+ | 10 mM, significant inhibition | Absidia sp. (in: Fungi) |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | activity of the dioscin-glycosidase is not affected by Na+, K+, and Mg2+ at 50 mM | Absidia sp. (in: Fungi) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
SDS-PAGE | Absidia sp. (in: Fungi) |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-O-[alpha-L-Rha-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin + 3 H2O | Absidia sp. (in: Fungi) | - |
D-glucopyranose + 2 L-rhamnopyranose + diosgenin | - |
? | |
3-O-[alpha-L-Rha-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin + 3 H2O | Absidia sp. (in: Fungi) d38 | - |
D-glucopyranose + 2 L-rhamnopyranose + diosgenin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Absidia sp. (in: Fungi) | - |
- |
- |
Absidia sp. (in: Fungi) d38 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Absidia sp. (in: Fungi) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.94 | - |
40°C, pH 5.0 | Absidia sp. (in: Fungi) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-O-[alpha-L-Ara-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin + 3 H2O | - |
Absidia sp. (in: Fungi) | D-glucopyranose + L-arabinose + L-rhamnopyranose + diosgenin | - |
? | |
3-O-[alpha-L-Ara-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin + 3 H2O | - |
Absidia sp. (in: Fungi) d38 | D-glucopyranose + L-arabinose + L-rhamnopyranose + diosgenin | - |
? | |
3-O-[alpha-L-Rha-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin + 3 H2O | - |
Absidia sp. (in: Fungi) | D-glucopyranose + 2 L-rhamnopyranose + diosgenin | - |
? | |
3-O-[alpha-L-Rha-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin + 3 H2O | 3-O-[alpha-L-Rha-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin is the the main dioscin in the rhizome of Dioscorea nipponica. The dioscin-glycosidase gradually hydrolyzes either the 3-O-alpha-L-(1->4)-Rha or the 3-O-alpha-L-(1->2)-Rha from dioscin into 3-O-alpha-L-Rha-(1->2)-beta-D-diosgenin or 3-O-alpha-L-Rha-(1->4)-beta-D-diosgenin, further rapidly hydrolyzes the other alpha-L-Rha into the main intermediate products of 3-O-beta-D-Glc-diosgenin, and subsequently hydrolyzes these intermediate product into aglycone as the final product | Absidia sp. (in: Fungi) | D-glucopyranose + 2 L-rhamnopyranose + diosgenin | - |
? | |
3-O-[alpha-L-Rha-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin + 3 H2O | - |
Absidia sp. (in: Fungi) d38 | D-glucopyranose + 2 L-rhamnopyranose + diosgenin | - |
? | |
3-O-[alpha-L-Rha-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin + 3 H2O | 3-O-[alpha-L-Rha-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin is the the main dioscin in the rhizome of Dioscorea nipponica. The dioscin-glycosidase gradually hydrolyzes either the 3-O-alpha-L-(1->4)-Rha or the 3-O-alpha-L-(1->2)-Rha from dioscin into 3-O-alpha-L-Rha-(1->2)-beta-D-diosgenin or 3-O-alpha-L-Rha-(1->4)-beta-D-diosgenin, further rapidly hydrolyzes the other alpha-L-Rha into the main intermediate products of 3-O-beta-D-Glc-diosgenin, and subsequently hydrolyzes these intermediate product into aglycone as the final product | Absidia sp. (in: Fungi) d38 | D-glucopyranose + 2 L-rhamnopyranose + diosgenin | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55000, SDS-PAGE | Absidia sp. (in: Fungi) |
Synonyms | Comment | Organism |
---|---|---|
dioscin glycosidase (aglycone-forming) | - |
Absidia sp. (in: Fungi) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Absidia sp. (in: Fungi) |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | 20°C: about 45% of maximal activity, 50°C: about 80% of maximal activity | Absidia sp. (in: Fungi) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Absidia sp. (in: Fungi) |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 6 | pH 4.0: about 95% of maximal activity, pH 6.0: about 90% of maximal activity | Absidia sp. (in: Fungi) |