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Literature summary for 3.2.1.188 extracted from
- Characterization of a membrane bound beta-glucosidase responsible for the activation of oat leaf saponins (1985), Phytochemistry, 24, 1941-1943.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Hg2+ | complete inhibition at 1 mM | Avena sativa |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.183 | - |
avenacoside B | at pH 6.5 and 25°C | Avena sativa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| tonoplast | bound to the tonoplast membrane | Avena sativa | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not influenced by 1 mM Cu2+ | Avena sativa |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 68000 | - |
x * 68000, SDS-PAGE | Avena sativa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| avenacoside A + H2O | Avena sativa | - |
26-desgluco-avenacoside A + D-glucose | - |
? | |
| avenacoside B + H2O | Avena sativa | - |
26-desgluco-avenacoside B + D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Avena sativa | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Avena sativa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Avena sativa | 4-nitrophenol + D-glucose | - |
? | |
| avenacoside A + H2O | - |
Avena sativa | 26-desgluco-avenacoside A + D-glucose | - |
? | |
| avenacoside B + H2O | - |
Avena sativa | 26-desgluco-avenacoside B + D-glucose | - |
? | |
| additional information | no activity with hederacoside C and cellobiose | Avena sativa | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 68000, SDS-PAGE | Avena sativa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Avena sativa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | - |
Avena sativa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.8 | 8 | 50% activity at pH 4.8 and 8.0, respectively | Avena sativa |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
at pH 6.5 and 25°C | Avena sativa | Hg2+ | |
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