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Literature summary for 3.2.1.187 extracted from

  • Fujita, K.; Sakamoto, S.; Ono, Y.; Wakao, M.; Suda, Y.; Kitahara, K.; Suganuma, T.
    Molecular cloning and characterization of a beta-L-arabinobiosidase in Bifidobacterium longum that belongs to a novel glycoside hydrolase family (2011), J. Biol. Chem., 286, 5143-5150.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.187

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(lambdaDE3) cells Bifidobacterium longum

Inhibitors

Inhibitors Comment Organism Structure
EDTA 1 mM EDTA decreases the enzymatic activity to 1.4% Bifidobacterium longum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0107
-
 4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline in 50 mM sodium acetate (pH 6.0), at 37°C Bifidobacterium longum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM Ca2+ increases the enzymatic activity by 43.8% Bifidobacterium longum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
156797
-
 x * 156797, calculated from amino acid sequence Bifidobacterium longum
157000
-
 x * 157000, SDS-PAGE Bifidobacterium longum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + H2O Bifidobacterium longum specific substrate 4-O-(beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose
-
 ?
4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + H2O Bifidobacterium longum JCM 1217 specific substrate 4-O-(beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose
-
 ?

Organism

Organism UniProt Comment Textmining
Bifidobacterium longum
-
-
-
Bifidobacterium longum JCM 1217
-
-
-

Purification (Commentary)

Purification (Comment) Organism
TALON metal affinity resin column chromatography Bifidobacterium longum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + H2O specific substrate Bifidobacterium longum 4-O-(beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose
-
 ?
4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + H2O specific substrate Bifidobacterium longum JCM 1217 4-O-(beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose
-
 ?
arabinan + H2O
-
 Bifidobacterium longum ?
-
 ?
arabinan + H2O
-
 Bifidobacterium longum JCM 1217 ?
-
 ?
carrot extension + H2O
-
 Bifidobacterium longum ?
-
 ?
carrot extension + H2O
-
 Bifidobacterium longum JCM 1217 ?
-
 ?
debranched arabinan + H2O
-
 Bifidobacterium longum ?
-
 ?
debranched arabinan + H2O
-
 Bifidobacterium longum JCM 1217 ?
-
 ?
additional information no activity with 4-nitrophenyl alpha-L-arabinofuranoside, 4-nitrophenyl alpha-L-arabinopyranoside, 4-nitrophenyl beta-L-arabinopyranoside, arabinoxylan, rhamnogalacturonan, 4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline and 4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(1->2)-(beta-L-arabinofuranosyl)-(1->2)-(beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline Bifidobacterium longum ?
-
 ?
additional information no activity with 4-nitrophenyl alpha-L-arabinofuranoside, 4-nitrophenyl alpha-L-arabinopyranoside, 4-nitrophenyl beta-L-arabinopyranoside, arabinoxylan, rhamnogalacturonan, 4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline and 4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(1->2)-(beta-L-arabinofuranosyl)-(1->2)-(beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline Bifidobacterium longum JCM 1217 ?
-
 ?
potato lectin + H2O
-
 Bifidobacterium longum ?
-
 ?

Subunits

Subunits Comment Organism
? x * 157000, SDS-PAGE Bifidobacterium longum
? x * 156797, calculated from amino acid sequence Bifidobacterium longum

Synonyms

Synonyms Comment Organism
beta-L-arabinobiosidase
-
 Bifidobacterium longum
hypBA2
-
 Bifidobacterium longum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Bifidobacterium longum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7
-
 4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline in 50 mM sodium acetate (pH 6.0), at 37°C Bifidobacterium longum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6
-
 Bifidobacterium longum