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Literature summary for 3.2.1.186 extracted from

  • Gus-Mayer, S.; Brunner, H.; Schneider-Poetsch, H.A.
    Rüdiger, W.: Avenacosidase from oat: purification, sequence analysis and biochemical characterization of a new member of the BGA family of beta-glucosidases (1994), Plant Mol. Biol., 26, 909-921.
    View publication on PubMed

General Stability

General Stability Organism
the enzyme is highly aggregated. It consists of 300-350 kDa aggregates and multimers thereof. Dissociation by freezing and thawing leads to complete loss of enzyme activity Avena sativa

Localization

Localization Comment Organism GeneOntology No. Textmining
plastid
-
Avena sativa 9536
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
x * 60000, the enzyme is highly aggregated. It consists of 300-350 kDa aggregates and multimers thereof Avena sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
avenacoside A + H2O Avena sativa the enzyme belongs to a preformed defence system of oat against fungal infection 26-deglucoavenacoside A + D-glucose
-
?
avenacoside B + H2O Avena sativa the enzyme belongs to a preformed defence system of oat against fungal infection 26-deglucoavenacoside B + D-glucose
-
?

Organism

Organism UniProt Comment Textmining
Avena sativa Q38786
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Avena sativa

Source Tissue

Source Tissue Comment Organism Textmining
seedling etiolated Avena sativa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
avenacoside A + H2O
-
Avena sativa 26-deglucoavenacoside A + D-glucose
-
?
avenacoside A + H2O the enzyme belongs to a preformed defence system of oat against fungal infection Avena sativa 26-deglucoavenacoside A + D-glucose
-
?
avenacoside B + H2O
-
Avena sativa 26-deglucoavenacoside B + D-glucose
-
?
avenacoside B + H2O the enzyme belongs to a preformed defence system of oat against fungal infection Avena sativa 26-deglucoavenacoside B + D-glucose
-
?
additional information 4-nitrophenyl derivatives serve as substrates, in decreasing order: beta-fucoside, beta-glucoside, beta-galactoside, beta-xyloside. The corresponding 2-nitrophenyl glycosides are less well accepted. No hydrolysis is found with alpha-glycosides and beta-thioglucoside Avena sativa ?
-
?

Subunits

Subunits Comment Organism
multimer x * 60000, the enzyme is highly aggregated. It consists of 300-350 kDa aggregates and multimers thereof Avena sativa

Synonyms

Synonyms Comment Organism
avenacosidase
-
Avena sativa