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Literature summary for 3.2.1.186 extracted from

  • Inoue, K.; Ebizuka, Y.
    Purification and characterization of furostanol glycoside 26-O-beta-glucosidase from Costus speciosus rhizomes (1996), FEBS Lett., 378, 157-160.
    View publication on PubMed
Search for more literature for 3.2.1.186

Inhibitors

Inhibitors Comment Organism Structure
beta-sitosterol
-
 Hellenia speciosa
cholesterol
-
 Hellenia speciosa
conduritol beta-epoxide
-
 Hellenia speciosa
D-glucono-1,5-lactone 0.25 mM, 50% inhibition Hellenia speciosa
diosgenin 0.079, 50% inhibition Hellenia speciosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 protogracillin pH 5.0, 40°C Hellenia speciosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
 1 * 54000 + 1 * 58000, SDS-PAGE Hellenia speciosa
58000
-
 1 * 54000 + 1 * 58000, SDS-PAGE Hellenia speciosa
110000
-
 gel filtration Hellenia speciosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Hellenia speciosa the enzyme is involved in the postharvest conversion of furostanol glycosides to spirostanol glycosides in Costus speciosus. Furostanol glycosides are the precursors of spirostane glycosides, containing a glucose unit attached to the C-26 hydroxyl of the aglycone ?
-
 ?

Organism

Organism UniProt Comment Textmining
Hellenia speciosa Q42707
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Hellenia speciosa

Source Tissue

Source Tissue Comment Organism Textmining
rhizome
-
 Hellenia speciosa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
325
-
 pH 5.0, 40°C Hellenia speciosa

Storage Stability

Storage Stability Organism
4°C, 20 mM potassium phosphate buffer, pH 7.0, stable for 3 weeks Hellenia speciosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-glucoside + H2O
-
 Hellenia speciosa 4-nitrophenol + beta-D-glucose
-
 ?
additional information the enzyme is involved in the postharvest conversion of furostanol glycosides to spirostanol glycosides in Costus speciosus. Furostanol glycosides are the precursors of spirostane glycosides, containing a glucose unit attached to the C-26 hydroxyl of the aglycone Hellenia speciosa ?
-
 ?
additional information no activity amygdalin, cellobiose, gentiobiose, octyl beta-D-glucoside or prunasin Hellenia speciosa ?
-
 ?
protodioscin + H2O
-
 Hellenia speciosa 26-deglucoprotodioscin + D-glucose
-
 ?
protogracillin the enzyme is highly specific for cleavage of the C26-bound glucose moiety of furostanol glycosides Hellenia speciosa 26-deglucoprotogracillin + D-glucose
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 54000 + 1 * 58000, SDS-PAGE Hellenia speciosa

Synonyms

Synonyms Comment Organism
F26G
-
 Hellenia speciosa
furostanol 26-O-beta-D-glucoside glucohydrolase
-
 Hellenia speciosa
furostanol glycoside 26-O-beta-D-glucosidase
-
 Hellenia speciosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 assay at Hellenia speciosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 assay at Hellenia speciosa
5 5.5 substrate: protogracillin Hellenia speciosa

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.4
-
 pH 5.0, 40°C Hellenia speciosa beta-sitosterol
2.4
-
 pH 5.0, 40°C Hellenia speciosa conduritol beta-epoxide
2.8
-
 pH 5.0, 40°C Hellenia speciosa cholesterol