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Literature summary for 3.2.1.185 extracted from

  • Nakamura, M.; Yasukawa, Y.; Furusawa, A.; Fuchiwaki, T.; Honda, T.; Okamura, Y.; Fujita, K.; Iwai, H.
    Functional characterization of unique enzymes in Xanthomonas euvesicatoria related to degradation of arabinofurano-oligosaccharides on hydroxyproline-rich glycoproteins (2018), PLoS ONE, 13, e0201982 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.185

Cloned(Commentary)

Cloned (Comment) Organism
gene XCV2724, belongs to operon xehypBA2-AA containing genes XCV2724, XCV2728, and XCV2729, DNA and amino acid sequence determination and analysis, and genetic structure, recombinant expression of wild-type and mutant enzymes without the signal peptide in Escherichia coli strain BL21(DE3), recombinant expression of the operon in transgenic Solanum lycopersicum strain Micro-Tom Xanthomonas euvesicatoria

Protein Variants

Protein Variants Comment Organism
additional information construction of a single-gene deletion mutant, DELTAxehypBA1 and a triple-gene deletion mutant DELTAxehypBA1-BA2-AA. The mutants remain pathogenic, and mutations of the operon or the single genes XCV2724, XCV2728, and XCV2729 have no effect on nonhost resistance, either, indicating that these three enzymes are not involved in either pathogenicity or nonhost resistance reactions Xanthomonas euvesicatoria

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information the enzyme contains a 43 amino-acid signal peptide Xanthomonas euvesicatoria
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose + H2O Xanthomonas euvesicatoria
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 2 beta-L-arabinofuranose
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 ?
beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose + H2O Xanthomonas euvesicatoria 85-10
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 2 beta-L-arabinofuranose
-
 ?
beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose + H2O Xanthomonas euvesicatoria UPB139
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 2 beta-L-arabinofuranose
-
 ?
beta-L-arabinofuranosyl-L-hydroxyproline + H2O Xanthomonas euvesicatoria
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 beta-L-arabinofuranose + L-hydroxyproline
-
 ?
beta-L-arabinofuranosyl-L-hydroxyproline + H2O Xanthomonas euvesicatoria 85-10
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 beta-L-arabinofuranose + L-hydroxyproline
-
 ?
beta-L-arabinofuranosyl-L-hydroxyproline + H2O Xanthomonas euvesicatoria UPB139
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 beta-L-arabinofuranose + L-hydroxyproline
-
 ?

Organism

Organism UniProt Comment Textmining
Xanthomonas euvesicatoria Q3BS08
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Xanthomonas euvesicatoria 85-10 Q3BS08
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Xanthomonas euvesicatoria UPB139 Q3BS08
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose + H2O
-
 Xanthomonas euvesicatoria 2 beta-L-arabinofuranose
-
 ?
beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose + H2O
-
 Xanthomonas euvesicatoria 85-10 2 beta-L-arabinofuranose
-
 ?
beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose + H2O
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 Xanthomonas euvesicatoria UPB139 2 beta-L-arabinofuranose
-
 ?
beta-L-arabinofuranosyl-L-hydroxyproline + H2O
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 Xanthomonas euvesicatoria beta-L-arabinofuranose + L-hydroxyproline
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 ?
beta-L-arabinofuranosyl-L-hydroxyproline + H2O
-
 Xanthomonas euvesicatoria 85-10 beta-L-arabinofuranose + L-hydroxyproline
-
 ?
beta-L-arabinofuranosyl-L-hydroxyproline + H2O
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 Xanthomonas euvesicatoria UPB139 beta-L-arabinofuranose + L-hydroxyproline
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 ?
additional information substrate specificity, overview. Enzyme XeHypBA1 prefers Ara-Hyp as a substrate and liberates L-arabinose and is only slightly active on beta-Ara2, but does not use Ara4-Hyp and Ara3-Hyp at all. Ara2-Hyp is completely degraded in its dansylated form, but only partially when unmodified Xanthomonas euvesicatoria ?
-
 ?
additional information substrate specificity, overview. Enzyme XeHypBA1 prefers Ara-Hyp as a substrate and liberates L-arabinose and is only slightly active on beta-Ara2, but does not use Ara4-Hyp and Ara3-Hyp at all. Ara2-Hyp is completely degraded in its dansylated form, but only partially when unmodified Xanthomonas euvesicatoria 85-10 ?
-
 ?
additional information substrate specificity, overview. Enzyme XeHypBA1 prefers Ara-Hyp as a substrate and liberates L-arabinose and is only slightly active on beta-Ara2, but does not use Ara4-Hyp and Ara3-Hyp at all. Ara2-Hyp is completely degraded in its dansylated form, but only partially when unmodified Xanthomonas euvesicatoria UPB139 ?
-
 ?

Synonyms

Synonyms Comment Organism
GH 127 beta-L-arabinofuranosidase
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 Xanthomonas euvesicatoria
GH127 beta-L-arabinofuranosidase
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 Xanthomonas euvesicatoria
xcv2724
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 Xanthomonas euvesicatoria
XeHypBA1
-
 Xanthomonas euvesicatoria

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Xanthomonas euvesicatoria

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
 assay at Xanthomonas euvesicatoria

Expression

Organism Comment Expression
Xanthomonas euvesicatoria the operon is upregulated by transcription factor HrpX up

General Information

General Information Comment Organism
evolution the enzyme belongs to the glycosyl hydrolase family 127, GH127 Xanthomonas euvesicatoria
malfunction single deletion mutants of genes XCV2724, XCV2728, and XCV2729 and the triple deletion mutant remain pathogenic, and mutations of the operon or the single genes have no effect on nonhost resistance, either, indicating that these three enzymes are not involved in either pathogenicity or nonhost resistance reactions Xanthomonas euvesicatoria
physiological function enzymes XCV2724, XCV2728, and XCV2729 in Xanthomonas euvesicatoria degrade the arabinofuranooligosaccharides present on hydroxyproline (Hyp)-rich glycoproteins (HRGPs) such as extensin and solanaceous lectins in plant cell walls. These enzymes work coordinately to degrade the oligosaccharides. The main oligosaccharide structure of Ara4-Hyp on the HRGPs is degraded to Ara3-Hyp, then to Ara-Hyp, and finally to Ara monosaccharides by the action of these three enzymes. Structure of arabino-oligosaccharides on extensin and solanaceous lectins in plant cell walls, overview. HRGPs containing oligosaccharide substrates have been reported to contribute to plant defense. The enzyme is not involved in either pathogenicity or nonhost resistance reactions Xanthomonas euvesicatoria