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Literature summary for 3.2.1.185 extracted from

  • Fujita, K.; Takashi, Y.; Obuchi, E.; Kitahara, K.; Suganuma, T.
    Characterization of a novel ?-L-Arabinofuranosidase in Bifidobacterium longum: functional elucidation of A DUF1680 family member (2011), J. Biol. Chem., 286, 38079-38085.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.185

Protein Variants

Protein Variants Comment Organism
E322A 1.5% of wild-type activity Bifidobacterium longum
E338A 16% of wild-type activity Bifidobacterium longum
E366A 0.0013% of wild-type activity Bifidobacterium longum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.31
-
 beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-L-hydroxyproline wild-type, pH 4.5, 37°C Bifidobacterium longum
0.43
-
 beta-L-arabinofuranosyl-L-hydroxyproline wild-type, pH 4.5, 37°C Bifidobacterium longum
0.85
-
 beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose wild-type, pH 4.5, 37°C Bifidobacterium longum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
74000
-
 x * 74000, SDS-PAGE, x * 74319, calculated Bifidobacterium longum
74319
-
 x * 74000, SDS-PAGE, x * 74319, calculated Bifidobacterium longum

Organism

Organism UniProt Comment Textmining
Bifidobacterium longum E8MGH8
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00003
-
 mutant E366A, pH 4.5, 37°C Bifidobacterium longum
0.032
-
 mutant E322A, pH 4.5, 37°C Bifidobacterium longum
0.34
-
 mutant E338A, pH 4.5, 37°C Bifidobacterium longum
2.1
-
 wild-type, pH 4.5, 37°C Bifidobacterium longum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose + H2O
-
 Bifidobacterium longum 2 beta-L-arabinofuranose enzyme liberates L-arabinose from the L-arabinofuranose (Ara)-beta1,2-Ara disaccharide ?
beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-L-hydroxyproline + H2O
-
 Bifidobacterium longum 2 beta-L-arabinofuranose + L-hydroxyproline
-
 ?
beta-L-arabinofuranosyl-L-hydroxyproline + H2O
-
 Bifidobacterium longum beta-L-arabinofuranose + L-hydroxyproline
-
 ?
additional information in addition, enzyme transglycosylates 1-alkanols with retention of the anomeric configuration Bifidobacterium longum ?
-
 ?
additional information no substrates: 4-nitrophenyl-alpha-L-arabinopyranoside, 4-nitrophenyl-alpha-L-arabinofuranoside, 4-nitrophenyl-beta-L-arabinopyranoside, or (beta-L-arabinofuranosyl)4-L-hydroxyproline Bifidobacterium longum ?
-
 ?

Subunits

Subunits Comment Organism
? x * 74000, SDS-PAGE, x * 74319, calculated Bifidobacterium longum

Synonyms

Synonyms Comment Organism
beta-L-arabinofuranosidase
-
 Bifidobacterium longum
HypBA1
-
 Bifidobacterium longum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35 40
-
 Bifidobacterium longum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.013
-
 beta-L-arabinofuranosyl-L-hydroxyproline wild-type, pH 4.5, 37°C Bifidobacterium longum
2
-
 beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose wild-type, pH 4.5, 37°C Bifidobacterium longum
6.3
-
 beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-L-hydroxyproline wild-type, pH 4.5, 37°C Bifidobacterium longum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Bifidobacterium longum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.03
-
 beta-L-arabinofuranosyl-L-hydroxyproline wild-type, pH 4.5, 37°C Bifidobacterium longum
2.3
-
 beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose wild-type, pH 4.5, 37°C Bifidobacterium longum
20
-
 beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-L-hydroxyproline wild-type, pH 4.5, 37°C Bifidobacterium longum