Application | Comment | Organism |
---|---|---|
biotechnology | a GNE-deficient HEK293 cell line proves its potential for the production of glycoproteins with modified sialylation, gaining therapeutic and diagnostic glycoproteins, and efficient application of metabolic oligosaccharide engineering | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
gene GNE | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | efficient metabolic oligosaccharide engineering of glycoproteins by GNE enzyme knockdown by specific shRNAs in HEK293 cells. Stable GNE knockout via RNAi dramatically increases incorporation of N-acetylmannosamine analogues into glycoproteins of HEK293 cells. By means of these GNE-deficient cells highly sialylated glycoproteins can efficiently be decorated with reactive functional groups, which can be employed in bioorthogonal functionalization strategies for fluorescence labelling or biotinylation | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-alpha-D-glucosamine + H2O | Homo sapiens | - |
N-acetyl-D-mannosamine + UDP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HEK-293 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-alpha-D-glucosamine + H2O | - |
Homo sapiens | N-acetyl-D-mannosamine + UDP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GNE | - |
Homo sapiens |
UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
metabolism | key enzyme in the sialic acid biosynthetic pathway | Homo sapiens |