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Literature summary for 3.2.1.183 extracted from

  • Moeller, H.; Boehrsch, V.; Lucka, L.; Hackenberger, C.P.; Hinderlich, S.
    Efficient metabolic oligosaccharide engineering of glycoproteins by UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase (GNE) knock-down (2011), Mol. Biosyst., 7, 2245-2251.
    View publication on PubMed

Application

Application Comment Organism
biotechnology a GNE-deficient HEK293 cell line proves its potential for the production of glycoproteins with modified sialylation, gaining therapeutic and diagnostic glycoproteins, and efficient application of metabolic oligosaccharide engineering Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
gene GNE Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information efficient metabolic oligosaccharide engineering of glycoproteins by GNE enzyme knockdown by specific shRNAs in HEK293 cells. Stable GNE knockout via RNAi dramatically increases incorporation of N-acetylmannosamine analogues into glycoproteins of HEK293 cells. By means of these GNE-deficient cells highly sialylated glycoproteins can efficiently be decorated with reactive functional groups, which can be employed in bioorthogonal functionalization strategies for fluorescence labelling or biotinylation Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-alpha-D-glucosamine + H2O Homo sapiens
-
N-acetyl-D-mannosamine + UDP
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293 cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-alpha-D-glucosamine + H2O
-
Homo sapiens N-acetyl-D-mannosamine + UDP
-
?

Synonyms

Synonyms Comment Organism
GNE
-
Homo sapiens
UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase
-
Homo sapiens

General Information

General Information Comment Organism
metabolism key enzyme in the sialic acid biosynthetic pathway Homo sapiens