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Literature summary for 3.2.1.182 extracted from

  • Hannemann, L.; Lucaciu, C.R.; Sharma, S.; Rattei, T.; Mayer, K.F.X.; Gierl, A.; Frey, M.
    A promiscuous beta-glucosidase is involved in benzoxazinoid deglycosylation in Lamium galeobdolon (2018), Phytochemistry, 156, 224-233 .
    View publication on PubMed
Search for more literature for 3.2.1.182

Cloned(Commentary)

Cloned (Comment) Organism
gene Bglu1, quantitative RT-PCR expression analysis Lamium galeobdolon
gene Bglu2, quantitative RT-PCR expression analysis Lamium galeobdolon
gene Bglu3, quantitative RT-PCR expression analysis Lamium galeobdolon
gene Bglu4, quantitative RT-PCR expression analysis Lamium galeobdolon

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DIBOA beta-D-glucoside + H2O Lamium galeobdolon i.e. benzoxazinoid GDIBOA, a DIBOA glucoside DIBOA + beta-D-glucose
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 ?

Organism

Organism UniProt Comment Textmining
Lamium galeobdolon A0A3G1VCA8 i.e. Galeopsis galeobdolon
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Lamium galeobdolon A0A3G1VCA9 i.e. Galeopsis galeobdolon
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Lamium galeobdolon A0A3G1VCB1 i.e. Galeopsis galeobdolon
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Lamium galeobdolon A0A3G1VCB6 i.e. Galeopsis galeobdolon
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Source Tissue

Source Tissue Comment Organism Textmining
flower
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 Lamium galeobdolon
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leaf
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 Lamium galeobdolon
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additional information quantitative tissue expression analysis of LgGLU isozymes, LgGLU1-4, overview. Isozyme LgGLU3 shows the highest expression, LgGLU4 the lowest Lamium galeobdolon
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root
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 Lamium galeobdolon
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl-beta-D-cellobioside + H2O
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 Lamium galeobdolon 4-nitrophenol + D-cellobiose
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 ?
4-nitrophenyl-beta-D-fucopyranoside + H2O
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 Lamium galeobdolon 4-nitrophenol + D-fucopyranose
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 ?
4-nitrophenyl-beta-D-glucopyranoside + H2O
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 Lamium galeobdolon 4-nitrophenol + D-glucopyranose
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 ?
7-methoxy-2,4-dihydroxy-1,4-benzoxazin-3-one beta-D-glucoside + H2O i.e. benzoxazinoid GDIMBOA, a DIBOA glucoside Lamium galeobdolon 2-hydroxy-7-methoxy-3,4-dihydro-2H-1,4-benzoxazin + beta-D-glucose
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 ?
DIBOA beta-D-glucoside + H2O i.e. benzoxazinoid GDIBOA, a DIBOA glucoside Lamium galeobdolon DIBOA + beta-D-glucose
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 ?
additional information GDIBOA BGLUs of the dicots are promiscuous enzymes Lamium galeobdolon ?
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 ?
additional information substrate specificity of LgGLU isozymes with different substrates: artificial p-nitrophenyl glycoside substrates, benzoxazinoids, iridoids, cyanogenic glycoside, isoflavonoid glucosides, hydroquinone glucoside, and cytokinin glucoside, overview. Besides benzoxazinoid GDIBOA, the enzyme catalyzes the hydrolsis of oleuropein, dhurrin, daidzin, and genistin. The enzyme does not catalyze the hydrolysis of benzoxazinoid GDIMBOA, harpagide, arbutin, geniposide, trans-zeatin-O-glucoside, phloridzin, and 4-nitrophenyl-beta-D-mannopyranoside. GDIBOA BGLUs of the dicots are promiscuous enzymes Lamium galeobdolon ?
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 ?
additional information substrate specificity of LgGLU isozymes with different substrates: artificial p-nitrophenyl glycoside substrates, benzoxazinoids, iridoids, cyanogenic glycoside, isoflavonoid glucosides, hydroquinone glucoside, and cytokinin glucoside, overview. Besides benzoxazinoid GDIBOA, the enzyme catalyzes the hydrolsis of oleuropein, dhurrin, daidzin, and phloridzin. The enzyme does not catalyze the hydrolysis of benzoxazinoid GDIMBOA, harpagide, arbutin, genistin, geniposide, trans-zeatin-O-glucoside, and 4-nitrophenyl-beta-D-mannopyranoside. GDIBOA BGLUs of the dicots are promiscuous enzymes Lamium galeobdolon ?
-
 ?
additional information substrate specificity of LgGLU isozymes with different substrates: artificial p-nitrophenyl glycoside substrates, benzoxazinoids, iridoids, cyanogenic glycoside, isoflavonoid glucosides, hydroquinone glucoside, and cytokinin glucoside, overview. Besides the benzoxazinoids, the enzyme catalyzes the hydrolsis of oleuropein, geniposide, dhurrin, daidzin, phloridzin, genistin, and trans-zeatin-O-glucoside. The enzyme does not catalyze the hydrolysis of harpagide, arbutin, 4-nitrophenyl-beta-D-mannopyranoside, and 4-nitrophenyl-beta-D-fucopyranoside. GDIBOA BGLUs of the dicots are promiscuous enzymes Lamium galeobdolon ?
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 ?

Synonyms

Synonyms Comment Organism
LgGLU1
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 Lamium galeobdolon
LgGLU2
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 Lamium galeobdolon
LgGLU3
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 Lamium galeobdolon
LgGLU4
-
 Lamium galeobdolon
More cf. EC 3.2.1.21 Lamium galeobdolon

General Information

General Information Comment Organism
evolution the enzyme belongs to the glycosyl hydrolase family 1, GH1. Benzoxazinoids are characteristic defence compounds of the Poales but are also found in some unrelated dicots. The benzoxazinoid specific BGLUs have recently been identified for the grasses maize, wheat, rye and the Ranunculaceae Consolida orientalis. All enzymes share a general substrate ambiguity but differ in detailed substrate pattern. The isolation of the second dicot GDIBOA glucosidase LgGLU1 allowed it to analyse the phylogenetic relation of the distinct BGLUs also within dicots. The data revealed long periods of independent sequence evolution before speciation Lamium galeobdolon
evolution the enzyme belongs to the glycosyl hydrolase family 1, GH1. Benzoxazinoids are characteristic defence compounds of the Poales but are also found in some unrelated dicots. The benzoxazinoid specific BGLUs have recently been identified for the grasses maize, wheat, rye and the Ranunculaceae Consolida orientalis. All enzymes share a general substrate ambiguity but differ in detailed substrate pattern. The isolation of the second dicot GDIBOA glucosidase LgGLU1 allows it to analyse the phylogenetic relation of the distinct BGLUs also within dicots. The data reveals long periods of independent sequence evolution before speciation Lamium galeobdolon
physiological function the beta-glucosidases (BGLUs) of the glycosidase hydrolase family 1 in Lamium galeobdolon are involved in hydrolysis of the benzoxazinoid GDIBOA (2,4-dihydroxy-1,4-benzoxazin-3-one glucoside) Lamium galeobdolon