Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.182 extracted from

  • Nikus, J.; Esen, A.; Jonsson, L.
    Cloning of a plastidic rye (Secale cereale) beta-glucosidase cDNA and its expression in Escherichia coli (2003), Physiol. Plant., 118, 337-345.
No PubMed abstract available
Search for more literature for 3.2.1.182

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Secale cereale

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
 4-nitrophenyl beta-D-fucoside pH 5.8, 37°C, native protein Secale cereale
1.1
-
 4-nitrophenyl beta-D-fucoside pH 5.8, 37°C, recombinant protein Secale cereale
1.3
-
 4-nitrophenyl beta-D-galactoside pH 5.8, 37°C, native protein Secale cereale
1.3
-
 2-Nitrophenyl beta-D-glucoside pH 5.8, 37°C, recombinant protein Secale cereale
1.4
-
 2-Nitrophenyl beta-D-glucoside pH 5.8, 37°C, native protein Secale cereale
1.4
-
 DIMBOA beta-D-glucoside pH 5.8, 37°C, recombinant protein Secale cereale
1.5
-
 4-nitrophenyl beta-D-galactoside pH 5.8, 37°C, recombinant protein Secale cereale
2.1
-
 DIMBOA beta-D-glucoside pH 5.8, 37°C, native protein Secale cereale
2.4
-
 4-nitrophenyl beta-D-glucoside pH 5.8, 37°C, native protein Secale cereale
2.4
-
 4-nitrophenyl beta-D-glucoside pH 5.8, 37°C, recombinant protein Secale cereale
2.8
-
 DIBOA beta-D-glucoside pH 5.8, 37°C, recombinant protein Secale cereale
3.3
-
 DIBOA beta-D-glucoside pH 5.8, 37°C, native protein Secale cereale

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast putatively localised in chloroplasts Secale cereale 9507
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
 SDS-PAGE Secale cereale

Organism

Organism UniProt Comment Textmining
Secale cereale
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
coleoptile highest expression Secale cereale
-
mesocotyl highest expression Secale cereale
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-glucoside + H2O
-
 Secale cereale 2-nitrophenol + beta-D-glucose
-
 ?
4-nitrophenyl beta-D-fucoside + H2O
-
 Secale cereale 4-nitrophenol + beta-D-fucose
-
 ?
4-nitrophenyl beta-D-galactoside + H2O
-
 Secale cereale 4-nitrophenol + beta-D-galactose
-
 ?
4-nitrophenyl beta-D-glucoside + H2O
-
 Secale cereale 4-nitrophenol + beta-D-glucose
-
 ?
DIBOA beta-D-glucoside + H2O
-
 Secale cereale DIBOA + beta-D-glucose
-
 ?
DIMBOA beta-D-glucoside + H2O
-
 Secale cereale DIMBOA + beta-D-glucose
-
 ?

Synonyms

Synonyms Comment Organism
beta-glucosidase
-
 Secale cereale

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Secale cereale

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.3
-
 4-nitrophenyl beta-D-galactoside pH 5.8, 37°C, recombinant protein Secale cereale
3.5
-
 4-nitrophenyl beta-D-galactoside pH 5.8, 37°C, native protein Secale cereale
52
-
 4-nitrophenyl beta-D-glucoside pH 5.8, 37°C, native protein Secale cereale
66.8
-
 4-nitrophenyl beta-D-fucoside pH 5.8, 37°C, native protein Secale cereale
72.8
-
 4-nitrophenyl beta-D-glucoside pH 5.8, 37°C, recombinant protein Secale cereale
86.5
-
 4-nitrophenyl beta-D-fucoside pH 5.8, 37°C, recombinant protein Secale cereale
97.4
-
 DIMBOA beta-D-glucoside pH 5.8, 37°C, recombinant protein Secale cereale
119
-
 DIMBOA beta-D-glucoside pH 5.8, 37°C, native protein Secale cereale
159
-
 2-Nitrophenyl beta-D-glucoside pH 5.8, 37°C, native protein Secale cereale
169
-
 DIBOA beta-D-glucoside pH 5.8, 37°C, recombinant protein Secale cereale
172
-
 DIBOA beta-D-glucoside pH 5.8, 37°C, native protein Secale cereale
204.2
-
 2-Nitrophenyl beta-D-glucoside pH 5.8, 37°C, recombinant protein Secale cereale

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
 assay at Secale cereale