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Literature summary for 3.2.1.182 extracted from

  • Sue, M.; Yamazaki, K.; Yajima, S.; Nomura, T.; Matsukawa, T.; Iwamura, H.; Miyamoto, T.
    Molecular and structural characterization of hexameric beta-D-glucosidases in wheat and rye (2006), Plant Physiol., 141, 1237-1247.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.182

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Triticum aestivum
expressed in Escherichia coli as a His-tagged fusion protein Secale cereale

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure of hexameric TaGlu1b is determined at a resolution of 1.8 A in complex with DOMBOA. The N-terminal region is located at the dimer-dimer interface and plays a crucial role in hexamer formation Triticum aestivum

Protein Variants

Protein Variants Comment Organism
E191A mutant shows no activity Triticum aestivum
E191A mutant shows no activity Secale cereale
E407A mutant shows no activity Triticum aestivum
E407A mutant shows no activity Secale cereale
F198A kcat/Km values of ScGlu-F198A for DIMBOA-glucoside and DIBOA-glucoside decrease by a factor of 24- to 28fold as compared to wild-type ScGlu due to an increased Km, whereas the kcat value increases Secale cereale
F198A Km increased compared to wild-type, kcat decreased compared to wild-type Triticum aestivum
F471Y kcat values for DIBOA-glucoside increased, Km (DIBOA-glucoside) increased, Km (DIMBOA-glucoside) decreased, kcat values for DIMBOA-glucoside increased Secale cereale
F471Y Km increased compared to wild-type, kcat (DIBOA-glucoside) increased compared to wild-type, kcat (DIMBOA-glucoside or 4-nitrophenyl beta-D-glucoside) increased compared to wild-type Triticum aestivum
G464F kcat values for DIMBOA-glucoside and DIBOA-glucoside decreased, Km values increased Secale cereale
G464S kcat values for DIMBOA-glucoside and DIBOA-glucoside decreased, Km values increased Secale cereale
G464S/S465L kcat values for DIBOA-glucoside decreased, Km (DIBOA-glucoside) increased, Km (DIMBOA-glucoside) decreased, kcat values for DIMBOA-glucoside increased Secale cereale
additional information the N-terminal 25 residues of the mature TaGlu1a and TaGlu1b are replaced with the corresponding residues of Zea mays ZmGlu1, considering that this enzyme is known to exist as a dimer. The chimeric glucosidases (Zm-TaGlu1a and Zm-TaGlu1b) completely loses their ability to form a hexamer, which is confirmed by gel filtration chromatography Triticum aestivum
S464F Km increased compared to wild-type, kcat (DIBOA-glucoside) increased compared to wild-type, kcat (DIMBOA-glucoside or 4-nitrophenyl beta-D-glucoside) increased compared to wild-type Triticum aestivum
S465L kcat values for DIBOA-glucoside decreased, Km (DIBOA-glucoside) increased, Km (DIMBOA-glucoside) decreased, kcat values for DIMBOA-glucoside increased Secale cereale
Y378A kcat values for DIMBOA-glucoside and DIBOA-glucoside increased, Km values increased Secale cereale
Y378A Km increased compared to wild-type, kcat decreased compared to wild-type Triticum aestivum
Y378F kcat values for DIMBOA-glucoside and DIBOA-glucoside increased, Km values dereased Secale cereale
Y378F Km decreased compared to wild-type, kcat decreased compared to wild-type Triticum aestivum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 DIMBOA-glucoside pH 5.5, 30°C, mutant Y378F Triticum aestivum
0.29
-
 DIMBOA-glucoside pH 5.5, 30°C, wild-type Triticum aestivum
0.36
-
 DIMBOA-glucoside pH 5.5, 30°C, wild-type Triticum aestivum
0.39
-
 DIMBOA-glucoside pH 5.5, 30°C, wild-type Triticum aestivum
0.41
-
 DIBOA-glucoside pH 5.5, 30°C, mutant Y378F Triticum aestivum
0.52
-
 DIBOA-glucoside pH 5.5, 30°C, mutant Y378F Secale cereale
0.59
-
 DIMBOA-glucoside pH 5.5, 30°C, mutant G464S/S465L Secale cereale
0.7
-
 DIMBOA-glucoside pH 5.5, 30°C, mutant F471Y Triticum aestivum
0.76
-
 DIMBOA-glucoside pH 5.5, 30°C, mutant S465L Secale cereale
0.8
-
 DIBOA-glucoside pH 5.5, 30°C, wild-type Secale cereale
0.83
-
 DIBOA-glucoside pH 5.5, 30°C, mutant S465L Secale cereale
0.92
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, mutant F471Y Secale cereale
0.94
-
 DIMBOA-glucoside pH 5.5, 30°C, mutant G464S Secale cereale
1.02
-
 DIBOA-glucoside pH 5.5, 30°C, mutant G464S Secale cereale
1.04
-
 DIMBOA-glucoside pH 5.5, 30°C, mutant F471Y Secale cereale
1.05
-
 DIBOA-glucoside pH 5.5, 30°C, wild-type Triticum aestivum
1.07
-
 DIMBOA-glucoside pH 5.5, 30°C, mutant Y378F Secale cereale
1.19
-
 DIMBOA-glucoside pH 5.5, 30°C, mutant Y378A Triticum aestivum
1.23
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, mutant Y378F Triticum aestivum
1.3
-
 DIMBOA-glucoside pH 5.5, 30°C, wild-type Secale cereale
1.33
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, mutant Y378A Triticum aestivum
1.34
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, mutant Y378F Secale cereale
1.38
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, mutant G464S/S465L Secale cereale
1.4
-
 DIBOA-glucoside pH 5.5, 30°C, wild-type Triticum aestivum
1.42
-
 DIMBOA-glucoside pH 5.5, 30°C, mutant S464F Triticum aestivum
1.42
-
 DIMBOA-glucoside pH 5.5, 30°C, mutant Y378A Secale cereale
1.44
-
 DIBOA-glucoside pH 5.5, 30°C, wild-type Triticum aestivum
1.55
-
 DIBOA-glucoside pH 5.5, 30°C, mutant G464S/S465L Secale cereale
1.6
-
 DIBOA-glucoside pH 5.5, 30°C, mutant G464F Secale cereale
1.7
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, mutant G464F Secale cereale
1.75
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, wild-type Triticum aestivum
1.78
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, wild-type Secale cereale
1.85
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, wild-type Triticum aestivum
2.02
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, mutant Y378A Secale cereale
2.03
-
 DIBOA-glucoside pH 5.5, 30°C, mutant F471Y Secale cereale
2.03
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, mutant S465L Secale cereale
2.16
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, wild-type Triticum aestivum
2.31
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, mutant S464F Triticum aestivum
2.89
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, mutant G464S Secale cereale
2.89
-
 DIBOA-glucoside pH 5.5, 30°C, mutant S464F Triticum aestivum
2.93
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, mutant F471Y Triticum aestivum
3.05
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, mutant F198A Secale cereale
4.21
-
 DIBOA-glucoside pH 5.5, 30°C, mutant F471Y Triticum aestivum
5.12
-
 DIMBOA-glucoside pH 5.5, 30°C, mutant G464F Secale cereale
6.05
-
 DIBOA-glucoside pH 5.5, 30°C, mutant Y378A Triticum aestivum
7.99
-
 DIBOA-glucoside pH 5.5, 30°C, mutant Y378A Secale cereale
9.28
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, mutant F198A Triticum aestivum
27.5
-
 DIBOA-glucoside pH 5.5, 30°C, mutant F198A Triticum aestivum
35.7
-
 DIMBOA-glucoside pH 5.5, 30°C, mutant F198A Triticum aestivum
39.3
-
 DIMBOA-glucoside pH 5.5, 30°C, mutant F198A Secale cereale
44.1
-
 DIBOA-glucoside pH 5.5, 30°C, mutant F198A Secale cereale

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
 SDS-PAGE, native enzyme Triticum aestivum
59000
-
 calculated from cDNA, native enzyme Triticum aestivum
59160
-
 calculated from cDNA, native enzyme Triticum aestivum
59250
-
 calculated from cDNA, native enzyme Triticum aestivum
60000
-
 SDS-PAGE, native enzyme Triticum aestivum
64090
-
 mass-spectroscopy, His-tagged TaGlu1a Triticum aestivum
64140
-
 mass-spectroscopy, His-tagged TaGlu1a Triticum aestivum

Organism

Organism UniProt Comment Textmining
Secale cereale
-
-
-
Triticum aestivum
-
-
-
Triticum aestivum Q1XH05
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Triticum aestivum
-
 Secale cereale

Source Tissue

Source Tissue Comment Organism Textmining
shoot
-
 Triticum aestivum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-glucoside + H2O
-
 Triticum aestivum 4-nitrophenol + beta-D-glucose
-
 ?
4-nitrophenyl beta-D-glucoside + H2O
-
 Secale cereale 4-nitrophenol + beta-D-glucose
-
 ?
DIBOA-glucoside + H2O
-
 Triticum aestivum DIBOA + beta-D-glucose
-
 ?
DIBOA-glucoside + H2O
-
 Secale cereale DIBOA + beta-D-glucose
-
 ?
DIMBOA-glucoside + H2O
-
 Triticum aestivum DIMBOA + beta-D-glucose
-
 ?
DIMBOA-glucoside + H2O
-
 Secale cereale DIMBOA + beta-D-glucose
-
 ?

Subunits

Subunits Comment Organism
dimer minor bands in gel filtration are also observed Triticum aestivum
heterohexamer coexpression of TaGlu1a and TaGlu1b give seven bands on a native-PAGE gel, indicating the formation of both homo- and heterohexamers Triticum aestivum
heterohexamer coexpression of TaGlu1b and TaGlu1c give seven bands on a native-PAGE gel, indicating the formation of both homo- and heterohexamers Triticum aestivum
hexamer crystal structure, N-terminal region plays an important role in hexamer formation Triticum aestivum
hexamer major band in gel filtration shows activity with DIMBOA-glucose Triticum aestivum
homohexamer coexpression of TaGlu1a and TaGlu1b give seven bands on a native-PAGE gel, indicating the formation of both homo- and heterohexamers Triticum aestivum
homohexamer coexpression of TaGlu1b and TaGlu1c give seven bands on a native-PAGE gel, indicating the formation of both homo- and heterohexamers Triticum aestivum
monomer major band in gel filtration shows no activity with DIMBOA-glucose Triticum aestivum
tetramer minor bands in gel filtration are also observed Triticum aestivum

Synonyms

Synonyms Comment Organism
beta-glucosidase
-
 Triticum aestivum
beta-glucosidase
-
 Secale cereale
ScGlu
-
 Secale cereale
TaGlu1a
-
 Triticum aestivum
TaGlu1b
-
 Triticum aestivum
TaGlu1c
-
 Triticum aestivum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Triticum aestivum
30
-
 assay at Secale cereale

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.7
-
 DIBOA-glucoside pH 5.5, 30°C, mutant Y378F Triticum aestivum
4.7
-
 DIBOA-glucoside pH 5.5, 30°C, mutant Y378F Secale cereale
6.7
-
 DIBOA-glucoside pH 5.5, 30°C, mutant F198A Triticum aestivum
6.7
-
 DIBOA-glucoside pH 5.5, 30°C, mutant F198A Secale cereale
10.7
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, mutant F198A Triticum aestivum
10.7
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, mutant F198A Secale cereale
25.1
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, mutant Y378A Triticum aestivum
25.1
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, mutant Y378A Secale cereale
26.4
-
 DIBOA-glucoside pH 5.5, 30°C, mutant Y378A Triticum aestivum
26.4
-
 DIBOA-glucoside pH 5.5, 30°C, mutant Y378A Secale cereale
31.2
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, mutant S464F Triticum aestivum
31.2
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, mutant S464F Secale cereale
46.1
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, mutant Y378F Triticum aestivum
46.1
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, mutant Y378F Secale cereale
48.8
-
 DIBOA-glucoside pH 5.5, 30°C, wild-type Triticum aestivum
48.8
-
 DIBOA-glucoside pH 5.5, 30°C, wild-type Secale cereale
54.6
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, mutant F471Y Triticum aestivum
54.6
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, mutant F471Y Secale cereale
73.3
-
 DIMBOA-glucoside pH 5.5, 30°C, mutant S464F Triticum aestivum
73.3
-
 DIMBOA-glucoside pH 5.5, 30°C, mutant S464F Secale cereale
83.5
-
 DIBOA-glucoside pH 5.5, 30°C, mutant F471Y Triticum aestivum
83.5
-
 DIBOA-glucoside pH 5.5, 30°C, mutant F471Y Secale cereale
84
-
 DIBOA-glucoside pH 5.5, 30°C, mutant S464F Triticum aestivum
84
-
 DIBOA-glucoside pH 5.5, 30°C, mutant S464F Secale cereale
85.1
-
 DIMBOA-glucoside pH 5.5, 30°C, mutant Y378F Triticum aestivum
85.1
-
 DIMBOA-glucoside pH 5.5, 30°C, mutant Y378F Secale cereale
99.2
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, wild-type Triticum aestivum
99.2
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, wild-type Secale cereale
110
-
 DIMBOA-glucoside pH 5.5, 30°C, mutant F198A Triticum aestivum
110
-
 DIMBOA-glucoside pH 5.5, 30°C, mutant F198A Secale cereale
124
-
 DIMBOA-glucoside pH 5.5, 30°C, mutant Y378A Triticum aestivum
124
-
 DIMBOA-glucoside pH 5.5, 30°C, mutant Y378A Secale cereale
128
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, wild-type Triticum aestivum
137
-
 DIBOA-glucoside pH 5.5, 30°C, wild-type Triticum aestivum
192
-
 DIMBOA-glucoside pH 5.5, 30°C, mutant F471Y Triticum aestivum
192
-
 DIMBOA-glucoside pH 5.5, 30°C, mutant F471Y Secale cereale
214
-
 DIBOA-glucoside pH 5.5, 30°C, wild-type Triticum aestivum
235.9
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, wild-type Triticum aestivum
338
-
 DIMBOA-glucoside pH 5.5, 30°C, wild-type Triticum aestivum
338
-
 DIMBOA-glucoside pH 5.5, 30°C, wild-type Secale cereale
773
-
 DIMBOA-glucoside pH 5.5, 30°C, wild-type Triticum aestivum
1201
-
 DIMBOA-glucoside pH 5.5, 30°C, wild-type Triticum aestivum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 assay at Triticum aestivum
5.5
-
 assay at Secale cereale

Expression

Organism Comment Expression
Triticum aestivum compared to Taglu1a and Taglu1b mRNA level of Taglu1c is much lower in 48-h-old and 96-h-old wheat shoots up
Triticum aestivum Taglu1a is most highly expressed (67%) in 48-h-old wheat up
Triticum aestivum Taglu1b is highly expressed in 48-h-old and 96-h-old wheat shoots up

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.24
-
 DIBOA-glucoside pH 5.5, 30°C, mutant F198A Triticum aestivum
1.2
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, mutant F198A Triticum aestivum
2.72
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, mutant F198A Secale cereale
3.1
-
 DIMBOA-glucoside pH 5.5, 30°C, mutant F198A Triticum aestivum
4.36
-
 DIBOA-glucoside pH 5.5, 30°C, mutant Y378A Triticum aestivum
4.94
-
 DIMBOA-glucoside pH 5.5, 30°C, mutant F198A Secale cereale
5.28
-
 DIBOA-glucoside pH 5.5, 30°C, mutant F198A Secale cereale
11.5
-
 DIBOA-glucoside pH 5.5, 30°C, mutant Y378F Triticum aestivum
12.5
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, wild-type Secale cereale
13.5
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, mutant S464F Triticum aestivum
15
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, mutant G464F Secale cereale
18.6
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, mutant F471Y Triticum aestivum
18.9
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, mutant Y378A Triticum aestivum
19.8
-
 DIBOA-glucoside pH 5.5, 30°C, mutant F471Y Triticum aestivum
23.1
-
 DIBOA-glucoside pH 5.5, 30°C, mutant Y378F Secale cereale
23.6
-
 DIMBOA-glucoside pH 5.5, 30°C, mutant G464F Secale cereale
29.1
-
 DIBOA-glucoside pH 5.5, 30°C, mutant S464F Triticum aestivum
34.1
-
 DIBOA-glucoside pH 5.5, 30°C, mutant G464F Secale cereale
34.6
-
 DIBOA-glucoside pH 5.5, 30°C, wild-type Triticum aestivum
37.2
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, mutant S465L Secale cereale
37.5
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, mutant Y378F Triticum aestivum
42.9
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, mutant G464S Secale cereale
47
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, mutant Y378F Secale cereale
50.3
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, mutant F471Y Secale cereale
50.5
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, mutant Y378A Secale cereale
51.6
-
 DIMBOA-glucoside pH 5.5, 30°C, mutant S464F Triticum aestivum
53.6
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, wild-type Triticum aestivum
53.9
-
 DIBOA-glucoside pH 5.5, 30°C, mutant G464S/S465L Secale cereale
54.6
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, mutant G464S/S465L Secale cereale
56.1
-
 DIBOA-glucoside pH 5.5, 30°C, mutant S465L Secale cereale
59.3
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, wild-type Triticum aestivum
85.2
-
 DIBOA-glucoside pH 5.5, 30°C, mutant G464S Secale cereale
104
-
 DIMBOA-glucoside pH 5.5, 30°C, mutant Y378A Triticum aestivum
120
-
 DIBOA-glucoside pH 5.5, 30°C, mutant F471Y Secale cereale
122
-
 DIMBOA-glucoside pH 5.5, 30°C, wild-type Secale cereale
131
-
 DIBOA-glucoside pH 5.5, 30°C, wild-type Triticum aestivum
135
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C, wild-type Triticum aestivum
137
-
 DIBOA-glucoside pH 5.5, 30°C, mutant Y378A Secale cereale
148
-
 DIBOA-glucoside pH 5.5, 30°C, wild-type Secale cereale
149
-
 DIBOA-glucoside pH 5.5, 30°C, wild-type Triticum aestivum
182
-
 DIMBOA-glucoside pH 5.5, 30°C, mutant Y378F Secale cereale
244
-
 DIMBOA-glucoside pH 5.5, 30°C, mutant G464S Secale cereale
274
-
 DIMBOA-glucoside pH 5.5, 30°C, mutant F471Y Triticum aestivum
296
-
 DIMBOA-glucoside pH 5.5, 30°C, mutant S465L Secale cereale
309
-
 DIMBOA-glucoside pH 5.5, 30°C, mutant F471Y Secale cereale
488
-
 DIMBOA-glucoside pH 5.5, 30°C, mutant G464S/S465L Secale cereale
582
-
 DIMBOA-glucoside pH 5.5, 30°C, mutant Y378A Secale cereale
655
-
 DIMBOA-glucoside pH 5.5, 30°C, mutant Y378F Triticum aestivum
939
-
 DIMBOA-glucoside pH 5.5, 30°C, wild-type Triticum aestivum
1979
-
 DIMBOA-glucoside pH 5.5, 30°C, wild-type Triticum aestivum
4138
-
 DIMBOA-glucoside pH 5.5, 30°C, wild-type Triticum aestivum