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Literature summary for 3.2.1.181 extracted from

  • Yoshimi, Y.; Yaguchi, K.; Kaneko, S.; Tsumuraya, Y.; Kotake, T.
    Properties of two fungal endo-beta-1,3-galactanases and their synergistic action with an exo-beta-1,3-galactanase in degrading arabinogalactan-proteins (2017), Carbohydr. Res., 453-454, 26-35 .
    View publication on PubMed
Search for more literature for 3.2.1.181

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Pichia pastoris Neurospora crassa
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Pichia pastoris Aspergillus flavus
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Pichia pastoris Flammulina velutipes

Organism

Organism UniProt Comment Textmining
Aspergillus flavus
-
-
-
Flammulina velutipes
-
-
-
Neurospora crassa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme 1.2fold from Pichia pastoris by cation exchange chromatography and gel filtration Neurospora crassa
recombinant enzyme 1.6fold from Pichia pastoris by cation exchange chromatography and gel filtration Flammulina velutipes
recombinant enzyme 1.7fold from Pichia pastoris by cation exchange chromatography and gel filtration Aspergillus flavus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1
-
 purified recombinant enzyme, pH 5.0, 40°C Aspergillus flavus
15
-
 purified recombinant enzyme, pH 5.0, 45°C Neurospora crassa
17
-
 purified recombinant enzyme, pH 5.0, 40°C Flammulina velutipes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arabinogalactan + H2O from radish leaves Flammulina velutipes ?
-
 ?
arabinogalactan + H2O the enzyme specifically acts on beta-1,3-galactan within the plant proteoglycansin arabinogalactan-proteins (AGPs) in an endo-manner. The carbohydrate moieties of AGPs consist of beta-1,3-galactan main chains and beta-1,6-galactan side chains, to which other auxiliary sugars are attached. Enzyme Af3G hardly hydrolyze native arabinogalactan-proteins (AGPs) alone but acts synergistically with a fungal exo-beta-1,3-galactanase on the AGP Aspergillus flavus ?
-
 ?
arabinogalactan + H2O the enzyme specifically acts on beta-1,3-galactan within the plant proteoglycansin arabinogalactan-proteins (AGPs) in an endo-manner. The carbohydrate moieties of AGPs consist of beta-1,3-galactan main chains and beta-1,6-galactan side chains, to which other auxiliary sugars are attached. Enzyme NcEn3GAL hardly hydrolyze native arabinogalactan-proteins (AGPs) alone but acts synergistically with a fungal exo-beta-1,3-galactanase on the AGP. Very low activity on native gum arabic and the larch arabinogalactan Neurospora crassa ?
-
 ?
beta-1,3-galactan + H2O best substrate Neurospora crassa oligo-beta-(1->3)-D-galactose
-
 ?
beta-1,3-galactan + H2O best substrate Aspergillus flavus oligo-beta-(1->3)-D-galactose
-
 ?
beta-1,3-galactan + H2O best substrate Flammulina velutipes oligo-beta-(1->3)-D-galactose
-
 ?
beta-1,3-galactotetraose + H2O
-
 Flammulina velutipes 2 beta-1,3-galactobiose
-
 ?
beta-1,3-galactotetraose + H2O the enzyme seems to prefer hydrolyzing the central beta-1,3-glycosidic linkage of three in beta-1,3-galactotetraose Neurospora crassa 2 beta-1,3-galactobiose
-
 ?
beta-1,3-galactotetraose + H2O the enzyme seems to prefer hydrolyzing the central beta-1,3-glycosidic linkage of three in beta-1,3-galactotetraose Aspergillus flavus 2 beta-1,3-galactobiose
-
 ?
beta-1,4-galactan + H2O from lupin Neurospora crassa ?
-
 ?
beta-1,4-galactan + H2O from lupin Aspergillus flavus ?
-
 ?
beta-1,4-galactan + H2O from lupin Flammulina velutipes ?
-
 ?
gum arabic beta-1,3-galactan I + H2O
-
 Neurospora crassa ?
-
 ?
gum arabic beta-1,3-galactan I + H2O
-
 Aspergillus flavus ?
-
 ?
gum arabic beta-1,3-galactan I + H2O
-
 Flammulina velutipes ?
-
 ?
gum arabic beta-1,3-galactan II + H2O
-
 Neurospora crassa ?
-
 ?
gum arabic beta-1,3-galactan II + H2O
-
 Aspergillus flavus ?
-
 ?
gum arabic beta-1,3-galactan II + H2O
-
 Flammulina velutipes ?
-
 ?
larch arabinogalactan + H2O
-
 Neurospora crassa ?
-
 ?
larch arabinogalactan + H2O
-
 Aspergillus flavus ?
-
 ?
larch arabinogalactan + H2O
-
 Flammulina velutipes ?
-
 ?
larch beta-galactan I + H2O
-
 Neurospora crassa ?
-
 ?
larch beta-galactan I + H2O
-
 Aspergillus flavus ?
-
 ?
larch beta-galactan I + H2O
-
 Flammulina velutipes ?
-
 ?
larch beta-galactan II + H2O
-
 Neurospora crassa ?
-
 ?
larch beta-galactan II + H2O
-
 Aspergillus flavus ?
-
 ?
larch beta-galactan II + H2O
-
 Flammulina velutipes ?
-
 ?
larch beta-galactan III + H2O
-
 Neurospora crassa ?
-
 ?
larch beta-galactan III + H2O
-
 Aspergillus flavus ?
-
 ?
larch beta-galactan III + H2O
-
 Flammulina velutipes ?
-
 ?
additional information substrate specificity, overview. Gum arabic from acacia tree is a poor substrate Flammulina velutipes ?
-
 ?
additional information very low activity on native gum arabic and the larch arabinogalactan. Substrate specificity, overview Neurospora crassa ?
-
 ?
additional information very low activity on native gum arabic and the larch arabinogalactan. Substrate specificity, overview Aspergillus flavus ?
-
 ?
radish leaf arabinogalactan + H2O
-
 Neurospora crassa ?
-
 ?
radish leaf arabinogalactan + H2O
-
 Aspergillus flavus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 42000, recombinant enzyme, SDS-PAGE Neurospora crassa
? x * 37000, recombinant enzyme, SDS-PAGE Aspergillus flavus

Synonyms

Synonyms Comment Organism
Af3G
-
 Aspergillus flavus
endo-beta-1,3-galactanase
-
 Neurospora crassa
endo-beta-1,3-galactanase
-
 Aspergillus flavus
endo-beta-1,3-galactanase
-
 Flammulina velutipes
FvEn3GAL
-
 Flammulina velutipes
NcEn3GAL
-
 Neurospora crassa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 recombinant enzyme Aspergillus flavus
45
-
 assay at Flammulina velutipes
45
-
 recombinant enzyme Neurospora crassa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 60 activity range, profile overview, recombinant enzyme Neurospora crassa
10 60 40% of maximal activity at 50°C and 50% at 10°C, profile overview, recombinant enzyme Aspergillus flavus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 recombinant enzyme Neurospora crassa
5
-
 recombinant enzyme Aspergillus flavus
5
-
 assay at Flammulina velutipes

pH Range

pH Minimum pH Maximum Comment Organism
3 7 activity range, profile overview, recombinant enzyme Aspergillus flavus
3 7 over 50% of maximal activity within this range, inactive above pH 8.0, profile overview, recombinant enzyme Neurospora crassa

General Information

General Information Comment Organism
evolution the enzyme belongs to the glycosyl hydrolase family 16, GH16 Neurospora crassa
evolution the enzyme belongs to the glycosyl hydrolase family 16, GH16 Aspergillus flavus
evolution the enzyme belongs to the glycosyl hydrolase family 16, GH16 Flammulina velutipes
physiological function the endo-beta-1,3-galactanases presumably aid hydrolysis of arabinogalactan-proteins (AGPs) by internally breaking up AGPs, which creates more sites of attack for exo-beta-1,3-galactanase Neurospora crassa
physiological function the endo-beta-1,3-galactanases presumably aid hydrolysis of arabinogalactan-proteins (AGPs) by internally breaking up AGPs, which creates more sites of attack for exo-beta-1,3-galactanase Aspergillus flavus
physiological function the endo-beta-1,3-galactanases presumably aid hydrolysis of arabinogalactan-proteins (AGPs) by internally breaking up AGPs, which creates more sites of attack for exo-beta-1,3-galactanase Flammulina velutipes