Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Bacteroides fragilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | - |
Bacteroides fragilis | |
Ca2+ | - |
Bacteroides fragilis | |
Co2+ | - |
Bacteroides fragilis | |
Cu2+ | - |
Bacteroides fragilis | |
EDTA | - |
Bacteroides fragilis | |
Fe2+ | - |
Bacteroides fragilis | |
Fe3+ | - |
Bacteroides fragilis | |
Hg2+ | - |
Bacteroides fragilis | |
K+ | - |
Bacteroides fragilis | |
Mn2+ | - |
Bacteroides fragilis | |
Na+ | - |
Bacteroides fragilis | |
NH4+ | - |
Bacteroides fragilis | |
Zn2+ | - |
Bacteroides fragilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
4-methylumbelliferyl N-acetyl-alpha-D-neuraminic acid | at pH 6.5 and 50°C | Bacteroides fragilis | |
0.75 | - |
sialic acid | at pH 6.5 and 50°C | Bacteroides fragilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Bacteroides fragilis | |
Ni2+ | - |
Bacteroides fragilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
113600 | - |
gel filtration | Bacteroides fragilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacteroides fragilis | Q5L868 | - |
- |
Bacteroides fragilis NCTC 9343 | Q5L868 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-siallyllactose + H2O | - |
Bacteroides fragilis | sialic acid + lactose | - |
r | |
3'-siallyllactose + H2O | - |
Bacteroides fragilis NCTC 9343 | sialic acid + lactose | - |
r | |
4-methylumbelliferyl N-acetyl-alpha-D-neuraminic acid + H2O | highest activity | Bacteroides fragilis | ? | - |
? | |
6'-sialyllactose + H2O | - |
Bacteroides fragilis | sialic acid + lactose | - |
r | |
6'-sialyllactose + H2O | - |
Bacteroides fragilis NCTC 9343 | sialic acid + lactose | - |
r | |
colominic acid + H2O | - |
Bacteroides fragilis | ? | - |
? | |
colominic acid + H2O | - |
Bacteroides fragilis NCTC 9343 | ? | - |
? | |
additional information | the enzyme has no activities toward other substrates with beta-linkages or lacking sialic acid in the glycan moieties, including 4-methylumbelliferyl alpha- or 4-methylumbelliferyl beta-D-galactopyranoside, 4-nitrophenyl alpha- or 4-nitrophenyl beta-D-galactopyranoside, 2-nitrophenyl alpha-or 2-nitrophenyl beta-D-galactopyranoside, 3-nitrophenyl D-galactopyranoside, 4-nitrophenyl alpha- or 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl alpha- or 4-nitrophenyl beta-D-mannopyranoside, 4-nitrophenyl alpha-L-mannopyranoside, 4-nitrophenyl beta D-mannopyranoside, 4-nitrophenyl 2-acetylamino-2-deoxy-alpha- or 4-nitrophenyl 2-acetylamino-2-deoxy-beta-D-galactopyranoside, and 4-nitrophenyl 2-acetylamino-2-deoxy-beta-D-glucopyranoside | Bacteroides fragilis | ? | - |
? | |
additional information | the enzyme hydrolyzes alpha2-3- and alpha2-6-linked sialyllactose, as well as alpha2-8-linked disaccharides and polysaccharides, but with a preference for alpha2-8-linked saccharides over alpha2-3- and alpha2-6-linked substrates | Bacteroides fragilis | ? | - |
? | |
additional information | the enzyme has no activities toward other substrates with beta-linkages or lacking sialic acid in the glycan moieties, including 4-methylumbelliferyl alpha- or 4-methylumbelliferyl beta-D-galactopyranoside, 4-nitrophenyl alpha- or 4-nitrophenyl beta-D-galactopyranoside, 2-nitrophenyl alpha-or 2-nitrophenyl beta-D-galactopyranoside, 3-nitrophenyl D-galactopyranoside, 4-nitrophenyl alpha- or 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl alpha- or 4-nitrophenyl beta-D-mannopyranoside, 4-nitrophenyl alpha-L-mannopyranoside, 4-nitrophenyl beta D-mannopyranoside, 4-nitrophenyl 2-acetylamino-2-deoxy-alpha- or 4-nitrophenyl 2-acetylamino-2-deoxy-beta-D-galactopyranoside, and 4-nitrophenyl 2-acetylamino-2-deoxy-beta-D-glucopyranoside | Bacteroides fragilis NCTC 9343 | ? | - |
? | |
additional information | the enzyme hydrolyzes alpha2-3- and alpha2-6-linked sialyllactose, as well as alpha2-8-linked disaccharides and polysaccharides, but with a preference for alpha2-8-linked saccharides over alpha2-3- and alpha2-6-linked substrates | Bacteroides fragilis NCTC 9343 | ? | - |
? | |
sialic acid + lactose | the enzyme is able to transfer sialyl from sialic acid dimer or oligomer to lactose with high efficiency and strict alpha2-6 regioselectivity | Bacteroides fragilis | 6'-sialyllactose | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 57900, SDS-PAGE | Bacteroides fragilis |
Synonyms | Comment | Organism |
---|---|---|
GH33C | - |
Bacteroides fragilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Bacteroides fragilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 40 | the enzyme remains stable after 1 h at 20-40°C | Bacteroides fragilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
283.2 | - |
4-methylumbelliferyl N-acetyl-alpha-D-neuraminic acid | at pH 6.5 and 50°C | Bacteroides fragilis | |
329.6 | - |
sialic acid | at pH 6.5 and 50°C | Bacteroides fragilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Bacteroides fragilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | the enzyme shows more than 50% activity after 1 h at pH 5.0-6.0 | Bacteroides fragilis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
438 | - |
sialic acid | at pH 6.5 and 50°C | Bacteroides fragilis | |
4857.3 | - |
4-methylumbelliferyl N-acetyl-alpha-D-neuraminic acid | at pH 6.5 and 50°C | Bacteroides fragilis |