Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.18 extracted from

  • Sultana, I.; Yang, K.; Getie-Kebtie, M.; Couzens, L.; Markoff, L.; Alterman, M.; Eichelberger, M.C.
    Stability of neuraminidase in inactivated influenza vaccines (2014), Vaccine, 32, 2225-2230.
    View publication on PubMed

General Stability

General Stability Organism
incubation with 0.1% (w/v) RapiGest results in almost complete loss of enzyme activity influenza A virus
incubation with 0.1% (w/v) RapiGest results in approximately 80% loss of enzyme activity influenza A virus
the enzyme activity is not influenced by 0.1% (w/v) RapiGest influenza B virus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
fetuin + H2O influenza A virus
-
?
-
?
fetuin + H2O influenza B virus
-
?
-
?
fetuin + H2O influenza A virus H3N2
-
?
-
?

Organic Solvent Stability

Organic Solvent Comment Organism
deoxycholate incubation with 1% (w/v) deoxycholate results in no significant loss of enzyme activity influenza A virus
deoxycholate incubation with 1% (w/v) deoxycholate results in no significant loss of enzyme activity influenza B virus

Organism

Organism UniProt Comment Textmining
influenza A virus
-
-
-
influenza A virus H3N2
-
-
-
influenza B virus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
-
influenza A virus 4-methylumbelliferone + N-acetylneuraminic acid
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
-
influenza B virus 4-methylumbelliferone + N-acetylneuraminic acid
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
-
influenza A virus H3N2 4-methylumbelliferone + N-acetylneuraminic acid
-
?
fetuin + H2O
-
influenza A virus ?
-
?
fetuin + H2O
-
influenza B virus ?
-
?
fetuin + H2O
-
influenza A virus H3N2 ?
-
?

Synonyms

Synonyms Comment Organism
sialidase
-
influenza A virus
sialidase
-
influenza B virus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
25 the enzyme is stable at 4 and 25°C for 24 h. At 37°C, the activity is retained for at least 7 h in the monovalent bulks, but by 24 h it has about 50% of its original activity. Three freeze-thaw cycles lead to a loss of about 70% of enzyme activity influenza A virus
-
25 the enzyme is stable at 4 and 25°C for 24 h. At 37°C, the activity is retained for at least 7 h in the monovalent bulks, but by 24 h it has about 75% of its original activity. Three freeze-thaw cycles lead to a loss of about 20% of enzyme activity influenza A virus