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Literature summary for 3.2.1.18 extracted from
- Optimizing the biocatalytic productivity of an engineered sialidase from Trypanosoma rangeli for 3-sialyllactose production (2014), Enzyme Microb. Technol., 55, 85-93.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris | Trypanosoma rangeli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 72700 | - |
x * 72700, calculated from amino acid sequence | Trypanosoma rangeli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| fetuin + H2O | Trypanosoma rangeli | - |
? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trypanosoma rangeli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni2+ affinity column chromatography, and gel filtration | Trypanosoma rangeli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-sialyllactose + H2O | - |
Trypanosoma rangeli | sialic acid + lactose | - |
? | |
| casein glycomacropeptide + H2O | - |
Trypanosoma rangeli | sialic acid + ? | - |
? | |
| casein glycomacropeptide + lactose | - |
Trypanosoma rangeli | 3'-sialyllactose + ? | - |
? | |
| fetuin + H2O | - |
Trypanosoma rangeli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 72700, calculated from amino acid sequence | Trypanosoma rangeli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| sialidase | - |
Trypanosoma rangeli |
| Tr6 | - |
Trypanosoma rangeli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 65 | engineered free sialidase Tr6 is stable at 25°C for at least 24 h. At 45°C the enzyme is stable for at least 100 min. 2% of the initial trans-sialidase activity is retained after 5 min of incubation at 65°C | Trypanosoma rangeli |
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