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Literature summary for 3.2.1.18 extracted from

  • Morley, T.J.; Willis, L.M.; Whitfield, C.; Wakarchuk, W.W.; Withers, S.G.
    A new sialidase mechanism: bacteriophage K1F endo-sialidase is an inverting glycosidase (2009), J. Biol. Chem., 284, 17404-17410.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Escherichia phage K1F

Protein Variants

Protein Variants Comment Organism
E581A the mutation results in complete loss of sialidase activity Escherichia phage K1F

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia phage K1F endoNF is therefore an inverting glycosidase ?
-
?
polysialic acid + H2O Escherichia phage K1F
-
sialic acid
-
?

Organism

Organism UniProt Comment Textmining
Escherichia phage K1F
-
-
-

Purification (Commentary)

Purification (Comment) Organism
amylose resin column chromatography Escherichia phage K1F

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha(2-6)-sialyllactose + H2O
-
Escherichia phage K1F sialic acid + lactose
-
?
additional information endoNF is therefore an inverting glycosidase Escherichia phage K1F ?
-
?
additional information does not hydrolyze the monomeric and dimeric sialic acid with alpha-linked trifluoromethylumbelliferyl significantly over a 2 h period Escherichia phage K1F ?
-
?
polysialic acid + H2O
-
Escherichia phage K1F sialic acid
-
?
sialic acid tetramer with alpha-linked trifluoromethylumbelliferyl + H2O hydrolysis with formation of two alpha-linked trifluoromethylumbelliferyl glycoside-containing species, trifluoromethylumbelliferone and the TFMU sialoside monomer Escherichia phage K1F ?
-
?
sialic acid trimer with alpha-linked trifluoromethylumbelliferyl + H2O degraded with exclusive cleavage at the coumarin-sialoside bond Escherichia phage K1F ?
-
?

Synonyms

Synonyms Comment Organism
endo-sialidase
-
Escherichia phage K1F
endoNF
-
Escherichia phage K1F