Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Escherichia phage K1F |
Protein Variants | Comment | Organism |
---|---|---|
E581A | the mutation results in complete loss of sialidase activity | Escherichia phage K1F |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia phage K1F | endoNF is therefore an inverting glycosidase | ? | - |
? | |
polysialic acid + H2O | Escherichia phage K1F | - |
sialic acid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia phage K1F | - |
- |
- |
Purification (Comment) | Organism |
---|---|
amylose resin column chromatography | Escherichia phage K1F |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha(2-6)-sialyllactose + H2O | - |
Escherichia phage K1F | sialic acid + lactose | - |
? | |
additional information | endoNF is therefore an inverting glycosidase | Escherichia phage K1F | ? | - |
? | |
additional information | does not hydrolyze the monomeric and dimeric sialic acid with alpha-linked trifluoromethylumbelliferyl significantly over a 2 h period | Escherichia phage K1F | ? | - |
? | |
polysialic acid + H2O | - |
Escherichia phage K1F | sialic acid | - |
? | |
sialic acid tetramer with alpha-linked trifluoromethylumbelliferyl + H2O | hydrolysis with formation of two alpha-linked trifluoromethylumbelliferyl glycoside-containing species, trifluoromethylumbelliferone and the TFMU sialoside monomer | Escherichia phage K1F | ? | - |
? | |
sialic acid trimer with alpha-linked trifluoromethylumbelliferyl + H2O | degraded with exclusive cleavage at the coumarin-sialoside bond | Escherichia phage K1F | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
endo-sialidase | - |
Escherichia phage K1F |
endoNF | - |
Escherichia phage K1F |