Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Corynebacterium diphtheriae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | purified truncated NanH protein devoid of its signal sequence as a mature enzyme fused with the 6-His tag at the N-terminal region can cleave alpha-2,3- and alpah-2,6-linked sialic acid from sialic acid-containing substrates and is able to catalyse the transfer of sialic acid using several sialoconjugates as donor | Corynebacterium diphtheriae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.5 | - |
alpha(2-3)-sialyllactose | at 37°C | Corynebacterium diphtheriae | |
7 | - |
alpha(2-6)-sialyllactose | at 37°C | Corynebacterium diphtheriae | |
7.3 | - |
4-nitrophenyl alpha-sialoside | at 37°C | Corynebacterium diphtheriae | |
7.9 | - |
4-methylumbelliferyl alpha-sialoside | at 37°C | Corynebacterium diphtheriae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Corynebacterium diphtheriae | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66390 | - |
His-tagged enzyme, MALDI-TOF mass spectrometry | Corynebacterium diphtheriae |
70000 | - |
His-tagged enzyme, SDS-PAGE | Corynebacterium diphtheriae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium diphtheriae | C6JSW6 | - |
- |
Corynebacterium diphtheriae KCTC3075 | C6JSW6 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Q-Sepharose column chromatography | Corynebacterium diphtheriae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-(4-methylumbelliferyl) alpha-D-N-acetylneuraminic acid + H2O | - |
Corynebacterium diphtheriae | 4-methylumbelliferone + N-acetylneuraminic acid | - |
? | |
2'-(4-methylumbelliferyl) alpha-D-N-acetylneuraminic acid + H2O | - |
Corynebacterium diphtheriae KCTC3075 | 4-methylumbelliferone + N-acetylneuraminic acid | - |
? | |
4-methylumbelliferyl alpha-sialoside + H2O | 48.9% activity compared to alpha(2-6)-sialyllactose | Corynebacterium diphtheriae | ? | - |
? | |
4-methylumbelliferyl alpha-sialoside + H2O | 48.9% activity compared to alpha(2-6)-sialyllactose | Corynebacterium diphtheriae KCTC3075 | ? | - |
? | |
4-nitrophenyl alpha-sialoside + H2O | 65.5% activity compared to alpha(2-6)-sialyllactose | Corynebacterium diphtheriae | ? | - |
? | |
4-nitrophenyl alpha-sialoside + H2O | 65.5% activity compared to alpha(2-6)-sialyllactose | Corynebacterium diphtheriae KCTC3075 | ? | - |
? | |
alpha(2-3)-sialyllactose + H2O | 89.8% activity compared to alpha(2-6)-sialyllactose | Corynebacterium diphtheriae | sialic acid + lactose | - |
? | |
alpha(2-3)-sialyllactose + H2O | 89.8% activity compared to alpha(2-6)-sialyllactose | Corynebacterium diphtheriae KCTC3075 | sialic acid + lactose | - |
? | |
alpha(2-6)-sialyllactose + H2O | 100% activity | Corynebacterium diphtheriae | sialic acid + lactose | - |
? | |
alpha(2-6)-sialyllactose + H2O | 100% activity | Corynebacterium diphtheriae KCTC3075 | sialic acid + lactose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NanH | - |
Corynebacterium diphtheriae |
sialidase | - |
Corynebacterium diphtheriae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
around pH 7.0 | Corynebacterium diphtheriae |