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Literature summary for 3.2.1.18 extracted from

  • Kim, S.; Oh, D.B.; Kwon, O.; Kang, H.A.
    Identification and functional characterization of the NanH extracellular sialidase from Corynebacterium diphtheriae (2010), J. Biochem., 147, 523-533.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Corynebacterium diphtheriae

Protein Variants

Protein Variants Comment Organism
additional information purified truncated NanH protein devoid of its signal sequence as a mature enzyme fused with the 6-His tag at the N-terminal region can cleave alpha-2,3- and alpah-2,6-linked sialic acid from sialic acid-containing substrates and is able to catalyse the transfer of sialic acid using several sialoconjugates as donor Corynebacterium diphtheriae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.5
-
alpha(2-3)-sialyllactose at 37°C Corynebacterium diphtheriae
7
-
alpha(2-6)-sialyllactose at 37°C Corynebacterium diphtheriae
7.3
-
4-nitrophenyl alpha-sialoside at 37°C Corynebacterium diphtheriae
7.9
-
4-methylumbelliferyl alpha-sialoside at 37°C Corynebacterium diphtheriae

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
Corynebacterium diphtheriae
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66390
-
His-tagged enzyme, MALDI-TOF mass spectrometry Corynebacterium diphtheriae
70000
-
His-tagged enzyme, SDS-PAGE Corynebacterium diphtheriae

Organism

Organism UniProt Comment Textmining
Corynebacterium diphtheriae C6JSW6
-
-
Corynebacterium diphtheriae KCTC3075 C6JSW6
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Q-Sepharose column chromatography Corynebacterium diphtheriae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-(4-methylumbelliferyl) alpha-D-N-acetylneuraminic acid + H2O
-
Corynebacterium diphtheriae 4-methylumbelliferone + N-acetylneuraminic acid
-
?
2'-(4-methylumbelliferyl) alpha-D-N-acetylneuraminic acid + H2O
-
Corynebacterium diphtheriae KCTC3075 4-methylumbelliferone + N-acetylneuraminic acid
-
?
4-methylumbelliferyl alpha-sialoside + H2O 48.9% activity compared to alpha(2-6)-sialyllactose Corynebacterium diphtheriae ?
-
?
4-methylumbelliferyl alpha-sialoside + H2O 48.9% activity compared to alpha(2-6)-sialyllactose Corynebacterium diphtheriae KCTC3075 ?
-
?
4-nitrophenyl alpha-sialoside + H2O 65.5% activity compared to alpha(2-6)-sialyllactose Corynebacterium diphtheriae ?
-
?
4-nitrophenyl alpha-sialoside + H2O 65.5% activity compared to alpha(2-6)-sialyllactose Corynebacterium diphtheriae KCTC3075 ?
-
?
alpha(2-3)-sialyllactose + H2O 89.8% activity compared to alpha(2-6)-sialyllactose Corynebacterium diphtheriae sialic acid + lactose
-
?
alpha(2-3)-sialyllactose + H2O 89.8% activity compared to alpha(2-6)-sialyllactose Corynebacterium diphtheriae KCTC3075 sialic acid + lactose
-
?
alpha(2-6)-sialyllactose + H2O 100% activity Corynebacterium diphtheriae sialic acid + lactose
-
?
alpha(2-6)-sialyllactose + H2O 100% activity Corynebacterium diphtheriae KCTC3075 sialic acid + lactose
-
?

Synonyms

Synonyms Comment Organism
NanH
-
Corynebacterium diphtheriae
sialidase
-
Corynebacterium diphtheriae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
around pH 7.0 Corynebacterium diphtheriae