Cloned (Comment) | Organism |
---|---|
soluble, C-terminally His-tagged truncation mutant is functionally expressed in Spodoptera frugiperda Sf21 insect cells via baculovirus infection, protein is fused to the honeybee melittin secretion signal peptide, expression in Escherichia coli results in an inactive and insoluble enzyme | Human respirovirus 1 |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a soluble truncation mutant, amino acid residues 137-575, lacking the N-terminal hydrophobic membrane anchoring region, mutant shows increased sensitivity to inhibition by DANA | Human respirovirus 1 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,3-didehydro-2-deoxy-N-acetylneuraminic acid | i.e. DANA, specific, competitive neuraminidase inhibitor | Human respirovirus 1 | |
Cl- | recombinant mutant: increase in concentration from 5 to 150 mM results in a decrease in activity of 50%, native enzyme: increase in concentration from 10 to 150 mM results in a decrease in activity of 20% | Human respirovirus 1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.028 | - |
2'-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid | pH 4.6, 37°C, native wild-type enzyme | Human respirovirus 1 | |
0.117 | - |
2'-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid | pH 4.6, 37°C, recombinant mutant | Human respirovirus 1 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Human respirovirus 1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Human respirovirus 1 | - |
i.e. hPIV-1 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | N-glycosylated recombinant enzyme | Human respirovirus 1 |
Purification (Comment) | Organism |
---|---|
processed, recombinant His-tagged truncation mutant from insect cells by cation exchange and metal affinity chromatography, 1230fold | Human respirovirus 1 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.123 | - |
purified recombinant mutant | Human respirovirus 1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O | synthetic, fluorogenic substrate | Human respirovirus 1 | 4-methylumbelliferol + alpha-D-N-acetylneuraminic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
hemagglutinin-neuraminidase glycoprotein | - |
Human respirovirus 1 |
HN | - |
Human respirovirus 1 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Human respirovirus 1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
purified recombinant mutant | Human respirovirus 1 |
5 | - |
crude native enzyme | Human respirovirus 1 |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.028 | - |
2,3-didehydro-2-deoxy-N-acetylneuraminic acid | pH 4.6, 37°C, native wild-type enzyme | Human respirovirus 1 | |
0.117 | - |
2,3-didehydro-2-deoxy-N-acetylneuraminic acid | pH 4.6, 37°C, recombinant mutant | Human respirovirus 1 |