Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.18 extracted from

  • Wang, Z.M.; Tong, L.L.; Grant, D.; Cihlar, T.
    Expression and characterization of soluble human parainfluenza virus type 1 hemagglutinin-neuraminidase glycoprotein (2001), J. Virol. Methods, 98, 53-61.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
soluble, C-terminally His-tagged truncation mutant is functionally expressed in Spodoptera frugiperda Sf21 insect cells via baculovirus infection, protein is fused to the honeybee melittin secretion signal peptide, expression in Escherichia coli results in an inactive and insoluble enzyme Human respirovirus 1

Protein Variants

Protein Variants Comment Organism
additional information construction of a soluble truncation mutant, amino acid residues 137-575, lacking the N-terminal hydrophobic membrane anchoring region, mutant shows increased sensitivity to inhibition by DANA Human respirovirus 1

Inhibitors

Inhibitors Comment Organism Structure
2,3-didehydro-2-deoxy-N-acetylneuraminic acid i.e. DANA, specific, competitive neuraminidase inhibitor Human respirovirus 1
Cl- recombinant mutant: increase in concentration from 5 to 150 mM results in a decrease in activity of 50%, native enzyme: increase in concentration from 10 to 150 mM results in a decrease in activity of 20% Human respirovirus 1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.028
-
2'-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid pH 4.6, 37°C, native wild-type enzyme Human respirovirus 1
0.117
-
2'-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid pH 4.6, 37°C, recombinant mutant Human respirovirus 1

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Human respirovirus 1

Organism

Organism UniProt Comment Textmining
Human respirovirus 1
-
i.e. hPIV-1
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein N-glycosylated recombinant enzyme Human respirovirus 1

Purification (Commentary)

Purification (Comment) Organism
processed, recombinant His-tagged truncation mutant from insect cells by cation exchange and metal affinity chromatography, 1230fold Human respirovirus 1

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.123
-
purified recombinant mutant Human respirovirus 1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O synthetic, fluorogenic substrate Human respirovirus 1 4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
-
?

Synonyms

Synonyms Comment Organism
hemagglutinin-neuraminidase glycoprotein
-
Human respirovirus 1
HN
-
Human respirovirus 1

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Human respirovirus 1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
purified recombinant mutant Human respirovirus 1
5
-
crude native enzyme Human respirovirus 1

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.028
-
2,3-didehydro-2-deoxy-N-acetylneuraminic acid pH 4.6, 37°C, native wild-type enzyme Human respirovirus 1
0.117
-
2,3-didehydro-2-deoxy-N-acetylneuraminic acid pH 4.6, 37°C, recombinant mutant Human respirovirus 1