Cloned (Comment) | Organism |
---|---|
gene nanB, DNA and amino acid sequence determination and analysis, expression in Escherichia coli of the gene rendering the recombinant cells capable of utilizing several sialoconjugates when grown on a minimal medium with these conjugates as sole carbon source | Pasteurella multocida |
genes nanH, DNA and amino acid sequence determination and analysis, expression in Escherichia coli of the gene rendering the recombinant cells capable of utilizing several sialoconjugates when grown on a minimal medium with these conjugates as sole carbon source | Pasteurella multocida |
Protein Variants | Comment | Organism |
---|---|---|
additional information | insertional inactivation of nanH results in a mutant, which is not deficient in sialidase production, but exhibits reduced activity | Pasteurella multocida |
additional information | insertional inactivation of nanH, encoding the second sialidase of the organism, results in a mutant, which is not deficient in sialidase production, but exhibits reduced activity | Pasteurella multocida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.063 | - |
alpha(2-3')-sialyllactose | recombinant enzyme, pH 6.8, 37°C | Pasteurella multocida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
x * 80000, SDS-PAGE | Pasteurella multocida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pasteurella multocida | most strains of Pasteurella multocida produce sialidase activity which may contribute to colonization of the respiratory tract by the mucosal pathogen, or for production of lesions in an actiev infection | ? | - |
? | |
additional information | Pasteurella multocida | most strains of Pasteurella multocida produce sialidase activity which may contribute to colonization of the respiratory tract by the mucosal pathogen, or for production of lesions in an active infection | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pasteurella multocida | Q9EZV6 | gene nanH, from fowl cholera isolates, quantitiative relation of the 2 sialidase ativities, encoded by genes nanB and nanH, in different isolates, overview | - |
Pasteurella multocida | Q9EZV7 | gene nanB, from fowl cholera isolates, quantitiative relation of the 2 sialidase ativities, encoded by genes nanB and nanH, in different isolates, overview | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli, 950fold, by ammonium sulfate precipitation, and several chromatographic steps | Pasteurella multocida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
substrate specificity | Pasteurella multocida |
1.9 | - |
purified recombinant enzyme | Pasteurella multocida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha(2-3')-sialyllactose + H2O | nanH encoded enzyme is highly specific for the (2-3')-sialyllactose | Pasteurella multocida | sialic acid + lactose | - |
? | |
alpha(2-3')-sialyllactose + H2O | the nanB encoded enzyme hydrolyzes both 2-3'- and 2-6'-sialyllactose | Pasteurella multocida | sialic acid + lactose | - |
? | |
alpha(2-6')-sialyllactose + H2O | the nanB encoded enzyme hydrolyzes both (2-3')- and (2-6')-sialyllactose, the latter substrate is preferred | Pasteurella multocida | sialic acid + lactose | - |
? | |
alpha-acid glycoprotein + H2O | - |
Pasteurella multocida | sialic acid + ? | - |
? | |
apotransferrin + H2O | - |
Pasteurella multocida | sialic acid + ? | - |
? | |
bovine submaxillary gland mucin + H2O | - |
Pasteurella multocida | sialic acid + ? | - |
? | |
colominic acid + H2O | i.e. (2-8')-sialyllactose | Pasteurella multocida | sialic acid + lactose | - |
? | |
fetuin + H2O | - |
Pasteurella multocida | sialic acid + ? | - |
? | |
ganglioside GD1a + H2O | - |
Pasteurella multocida | sialic acid + ? | - |
? | |
ganglioside GT1b + H2O | - |
Pasteurella multocida | sialic acid + ? | - |
? | |
additional information | substrate specificity | Pasteurella multocida | ? | - |
? | |
additional information | most strains of Pasteurella multocida produce sialidase activity which may contribute to colonization of the respiratory tract by the mucosal pathogen, or for production of lesions in an actiev infection | Pasteurella multocida | ? | - |
? | |
additional information | most strains of Pasteurella multocida produce sialidase activity which may contribute to colonization of the respiratory tract by the mucosal pathogen, or for production of lesions in an active infection | Pasteurella multocida | ? | - |
? | |
additional information | substrate specificity, no or poor activity with ganglioside GM1, colominic acid, and alpha(2-6')-sialyllactose | Pasteurella multocida | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | x * 80000, SDS-PAGE | Pasteurella multocida |
Synonyms | Comment | Organism |
---|---|---|
sialidase | - |
Pasteurella multocida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Pasteurella multocida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | 6.8 | depending on the buffer system | Pasteurella multocida |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | - |
Pasteurella multocida |
5.5 | 9 | - |
Pasteurella multocida |