Literature summary for 3.2.1.179 extracted from

Itoh, T.; Hashimoto, W.; Mikami, B.; Murata, K.
Substrate recognition by unsaturated glucuronyl hydrolase from Bacillus sp. GL1 (2006), Biochem. Biophys. Res. Commun., 344, 253-262.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
vapor diffusion method, demonstration of substrate recognition mechanism of the unsaturated glucuronyl hydrolase by determining the X-ray crystallographic structure of its substrate-enzyme complexes (D88N/DELTAGlcA-Glc-Pha-Glc and D99N/DELTAGlcA-GlcNAc). The tetrasaccharide-enzyme complex demonstrates that at least four subsites are present in the active pocket	Bacillus sp. (in: Bacteria)

Inhibitors

Inhibitors	Comment	Organism	Structure
glycine	-	Bacillus sp. (in: Bacteria)

Organism

Organism	UniProt	Comment	Textmining
Bacillus sp. (in: Bacteria)	Q9RC92	-	-
Bacillus sp. (in: Bacteria) GL1	Q9RC92	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
beta-D-4-deoxy-DELTA-4,5-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp + H2O	the enzyme strongly recognizes DELTA-4,5-GlcA at subsite -1 through the formation of hydrogen bonds and stacking interactions, and prefers N-acetyl-D-galactosamine and glucose rather than N-acetyl-D-glucosamine as a residue accommodated in subsite +1, due to the steric hindrance	Bacillus sp. (in: Bacteria)	4-deoxy-L-threo-5-hexosulose-uronate + beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp	-	?
beta-D-4-deoxy-DELTA-4,5-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp + H2O	the enzyme strongly recognizes DELTA-4,5-GlcA at subsite -1 through the formation of hydrogen bonds and stacking interactions, and prefers N-acetyl-D-galactosamine and glucose rather than N-acetyl-D-glucosamine as a residue accommodated in subsite +1, due to the steric hindrance	Bacillus sp. (in: Bacteria) GL1	4-deoxy-L-threo-5-hexosulose-uronate + beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp	-	?

Synonyms

Synonyms	Comment	Organism
UGL	-	Bacillus sp. (in: Bacteria)

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
6.5	-	glycine	pH 6.5, 30°C	Bacillus sp. (in: Bacteria)

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Release 2023.1 (January 2023)