Literature summary for 3.2.1.178 extracted from

Hehemann, J.H.; Kelly, A.G.; Pudlo, N.A.; Martens, E.C.; Boraston, A.B.
Bacteria of the human gut microbiome catabolize red seaweed glycans with carbohydrate-active enzyme updates from extrinsic microbes (2012), Proc. Natl. Acad. Sci. USA, 109, 19786-19791.

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Cloned(Commentary)

Cloned (Comment)	Organism
gene BACPLE_01689, encoded in a polysaccharide utilization locus (PUL) obtained by horizontal gene transfer by the gut bacterium Bacteroides plebeius, DNA and amino acid sequence determination and analysis	Phocaeicola plebeius
gene BACPLE_01693, encoded in a polysaccharide utilization locus (PUL) obtained by horizontal gene transfer by the gut bacterium Bacteroides plebeius, DNA and amino acid sequence determination and analysis	Phocaeicola plebeius

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified enzyme comprising the catalytic domain (residues 25-599) in complex with product, X-ray diffraction structure determiation and analysis at 1.3 A resolution	Phocaeicola plebeius

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Phocaeicola plebeius	the enzyme degrades porphyran	?	-	?
additional information	Phocaeicola plebeius DSM 17135	the enzyme degrades porphyran	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Phocaeicola plebeius	B5CY92	gene BACPLE_01689	-
Phocaeicola plebeius	B5CY96	gene BACPLE_01693	-
Phocaeicola plebeius DSM 17135	B5CY92	gene BACPLE_01689	-
Phocaeicola plebeius DSM 17135	B5CY96	gene BACPLE_01693	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the enzyme degrades porphyran	Phocaeicola plebeius	?	-	?
additional information	the enzyme degrades porphyran	Phocaeicola plebeius DSM 17135	?	-	?

Subunits

Subunits	Comment	Organism
More	the enzyme shows a multidomain architecture consisting of an N-terminal (beta/alpha)8 barrel and two C-terminal beta-sandwich domains, which align via their convex faces to the exterior of the (beta/alpha)8 barrel and connect with two N-terminal beta-strands that become part of these C-terminal beta-sandwich domains. The distal part of the barrel lacks the helix alpha2 in the outer ring and parts of the surface loops, which form the rim in the canonical (beta/alpha)8 barrel fold	Phocaeicola plebeius

Synonyms

Synonyms	Comment	Organism
BpGH16B	-	Phocaeicola plebeius
BpGH86A	-	Phocaeicola plebeius
PORA	-	Phocaeicola plebeius
PORB	-	Phocaeicola plebeius

Expression

Organism	Comment	Expression
Phocaeicola plebeius	porphyran induces the gene locus encoding gene BACPLE_01689	up
Phocaeicola plebeius	porphyran induces the gene locus encoding gene BACPLE_01693	up

General Information

General Information	Comment	Organism
evolution	the enzyme belongs to the glycoside hydrolase family 16, GH16, encoded in a polysaccharide utilization locus (PUL) obtained by horizontal gene transfer by the gut bacterium Bacteroides plebeius, genetic analysis, overview	Phocaeicola plebeius
evolution	the enzyme belongs to the glycoside hydrolase family 86, GH86, encoded in a polysaccharide utilization locus (PUL) obtained by horizontal gene transfer by the gut bacterium Bacteroides plebeius, genetic analysis, overview	Phocaeicola plebeius
additional information	the enzyme shows a multidomain architecture consisting of an N-terminal (beta/alpha)8 barrel and two C-terminal beta-sandwich domains, which align via their convex faces to the exterior of the (beta/alpha)8 barrel and connect with two N-terminal beta-strands that become part of these C-terminal beta-sandwich domains. The distal part of the barrel lacks the helix alpha2 in the outer ring and parts of the surface loops, which form the rim in the canonical (beta/alpha)8 barrel fold	Phocaeicola plebeius

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Release 2023.1 (January 2023)