Cloned (Comment) | Organism |
---|---|
gene BACPLE_01689, encoded in a polysaccharide utilization locus (PUL) obtained by horizontal gene transfer by the gut bacterium Bacteroides plebeius, DNA and amino acid sequence determination and analysis | Phocaeicola plebeius |
gene BACPLE_01693, encoded in a polysaccharide utilization locus (PUL) obtained by horizontal gene transfer by the gut bacterium Bacteroides plebeius, DNA and amino acid sequence determination and analysis | Phocaeicola plebeius |
Crystallization (Comment) | Organism |
---|---|
purified enzyme comprising the catalytic domain (residues 25-599) in complex with product, X-ray diffraction structure determiation and analysis at 1.3 A resolution | Phocaeicola plebeius |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Phocaeicola plebeius | the enzyme degrades porphyran | ? | - |
? | |
additional information | Phocaeicola plebeius DSM 17135 | the enzyme degrades porphyran | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phocaeicola plebeius | B5CY92 | gene BACPLE_01689 | - |
Phocaeicola plebeius | B5CY96 | gene BACPLE_01693 | - |
Phocaeicola plebeius DSM 17135 | B5CY92 | gene BACPLE_01689 | - |
Phocaeicola plebeius DSM 17135 | B5CY96 | gene BACPLE_01693 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme degrades porphyran | Phocaeicola plebeius | ? | - |
? | |
additional information | the enzyme degrades porphyran | Phocaeicola plebeius DSM 17135 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme shows a multidomain architecture consisting of an N-terminal (beta/alpha)8 barrel and two C-terminal beta-sandwich domains, which align via their convex faces to the exterior of the (beta/alpha)8 barrel and connect with two N-terminal beta-strands that become part of these C-terminal beta-sandwich domains. The distal part of the barrel lacks the helix alpha2 in the outer ring and parts of the surface loops, which form the rim in the canonical (beta/alpha)8 barrel fold | Phocaeicola plebeius |
Synonyms | Comment | Organism |
---|---|---|
BpGH16B | - |
Phocaeicola plebeius |
BpGH86A | - |
Phocaeicola plebeius |
PORA | - |
Phocaeicola plebeius |
PORB | - |
Phocaeicola plebeius |
Organism | Comment | Expression |
---|---|---|
Phocaeicola plebeius | porphyran induces the gene locus encoding gene BACPLE_01689 | up |
Phocaeicola plebeius | porphyran induces the gene locus encoding gene BACPLE_01693 | up |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the glycoside hydrolase family 16, GH16, encoded in a polysaccharide utilization locus (PUL) obtained by horizontal gene transfer by the gut bacterium Bacteroides plebeius, genetic analysis, overview | Phocaeicola plebeius |
evolution | the enzyme belongs to the glycoside hydrolase family 86, GH86, encoded in a polysaccharide utilization locus (PUL) obtained by horizontal gene transfer by the gut bacterium Bacteroides plebeius, genetic analysis, overview | Phocaeicola plebeius |
additional information | the enzyme shows a multidomain architecture consisting of an N-terminal (beta/alpha)8 barrel and two C-terminal beta-sandwich domains, which align via their convex faces to the exterior of the (beta/alpha)8 barrel and connect with two N-terminal beta-strands that become part of these C-terminal beta-sandwich domains. The distal part of the barrel lacks the helix alpha2 in the outer ring and parts of the surface loops, which form the rim in the canonical (beta/alpha)8 barrel fold | Phocaeicola plebeius |