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Literature summary for 3.2.1.178 extracted from

  • Correc, G.; Hehemann, J.H.; Czjzek, M.; Helbert, W.
    Structural analysis of the degradation products of porphyran digested by Zobellia galactanivorans ?-porphyranase A (2011), Carbohydr. Polym., 83, 277-283.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged expression in Escherichia coli Zobellia galactanivorans

Organism

Organism UniProt Comment Textmining
Zobellia galactanivorans D7GXG0
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Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Zobellia galactanivorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
porphyran + H2O the backbone of porphyran consists largely (~70%) of (1->3)-linked beta-D-galactopyranose followed by (1->4)-linked alpha-L-galactopyranose-6-sulfate. The other 30% are mostly agarobiose repeating units of (1->3)-linked beta-D-galactopyranose followed by (1->4)-linked 3,6-anhydro-alpha-L-galactopyranose. Methylation of the D-galactose prevents its binding at position -1. The disaccharide alpha-L-galactopyranose-6-sulfate-(1->3)-beta-D-galactose and oligosaccharides of the alpha-L-Galp-6-sulfate-(1->3)-beta-D-Galp series are the major final product Zobellia galactanivorans alpha-L-galactopyranose-6-sulfate-(1->3)-beta-D-galactopyranoside + oligosaccharides derived from porphyran
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Synonyms

Synonyms Comment Organism
beta-porphyranase A
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Zobellia galactanivorans