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Literature summary for 3.2.1.177 extracted from

  • Matsuzawa, T.; Kimura, N.; Suenaga, H.; Yaoi, K.
    Screening, identification, and characterization of alpha-xylosidase from a soil metagenome (2016), J. Biosci. Bioeng., 122, 393-399 .
    View publication on PubMed
Search for more literature for 3.2.1.177

Activating Compound

Activating Compound Comment Organism Structure
additional information no effect on activity by 10 mM EDTA uncultured microorganism

Cloned(Commentary)

Cloned (Comment) Organism
gene meXyl31, DNA and amino acid sequence determination and analysis, phylogenetic tree, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) uncultured microorganism

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 94% inhibition at 5 mM uncultured microorganism
DMSO 4% inhibition at 10% and 13% at 25% uncultured microorganism
ethanol 30% inhibition at 10% and 75% at 25% uncultured microorganism
Fe2+ 24% inhibition at 5 mM uncultured microorganism
additional information no effect on activity by 10 mM EDTA uncultured microorganism
Zn2+ 85% inhibition at 5 mM uncultured microorganism

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.39
-
 4-nitrophenyl alpha-D-xylopyranoside pH 5.5, 45°C, recombinant enzyme uncultured microorganism
8 9 Isoprimeverose pH 5.5, 45°C, recombinant enzyme uncultured microorganism

Metals/Ions

Metals/Ions Comment Organism Structure
additional information poor effect on activity by 5 mM Mg2+, Mn2+ and Ca2+ uncultured microorganism

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
tamarind seed xyloglucan + H2O uncultured microorganism
-
 D-xylose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
uncultured microorganism A0A169PW02 isolated from a soil metagenomic library
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme MeXyl31 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography uncultured microorganism

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.2
-
 pH 5.5, 45°C, recombinant enzyme, substrate alpha-D-Xyl-(1->6)-beta-D-Glc-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc uncultured microorganism
0.22
-
 pH 5.5, 45°C, recombinant enzyme, substrate alpha-D-Xyl-(1->6)-beta-D-Glc-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc uncultured microorganism
0.54
-
 pH 5.5, 45°C, recombinant enzyme, substrate 4-nitrophenyl alpha-D-glucopyranoside uncultured microorganism
2
-
 pH 5.5, 45°C, recombinant enzyme, substrate isoprimeverose uncultured microorganism
69.5
-
 pH 5.5, 45°C, recombinant enzyme, substrate 4-nitrophenyl alpha-D-xylopyranoside uncultured microorganism

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-D-glucopyranoside + H2O low activity uncultured microorganism 4-nitrophenol + D-glucose
-
 ?
4-nitrophenyl alpha-D-xylopyranoside + H2O best substrate uncultured microorganism 4-nitrophenol + D-xylose
-
 ?
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc + H2O low activity uncultured microorganism alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-beta-D-Glc + D-xylose
-
 ?
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc + H2O low activity uncultured microorganism ?
-
 ?
isoprimeverose + H2O 6-O-(alpha-D-xylopyranosyl)-D-glucose or 6-O-alpha-D-xylopyranosyl-beta-D-glucopyranose, low activity uncultured microorganism D-xylose + D-glucose
-
 ?
additional information substrate specificty, overview. The enzyme is not active with 0.16% tamarind seed xyloglucan, maltose, 4-nitrophenyl-beta-D-glucopyranoside, 4-nitrophenyl-alpha-D-mannopyranoside, 4-nitrophenyl-beta-D-mannopyranoside, 4-nitrophenyl-alpha-L-rhamnopyranoside, 4-nitrophenyl-beta-D-xylopyranoside, 4-nitrophenyl-alpha-L-arabinofuranoside, 4-nitrophenyl-alpha-L-arabinopyranoside, 4-nitrophenyl-beta-L-arabinopyranoside, 4-nitrophenyl-alpha-L-fucopyranoside, 4-nitrophenyl-beta-D-fucopyranoside, 4-nitrophenyl-beta-L-fucopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside, and 4-nitrophenyl-beta-D-galactopyranoside uncultured microorganism ?
-
 ?
tamarind seed xyloglucan + H2O
-
 uncultured microorganism D-xylose + ?
-
 ?

Synonyms

Synonyms Comment Organism
MeXyl31
-
 uncultured microorganism

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 recombinant enzyme uncultured microorganism

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.69
-
 Isoprimeverose pH 5.5, 45°C, recombinant enzyme uncultured microorganism
154
-
 4-nitrophenyl alpha-D-xylopyranoside pH 5.5, 45°C, recombinant enzyme uncultured microorganism

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 recombinant enzyme uncultured microorganism

General Information

General Information Comment Organism
evolution the enzyme belongs to the glycosylhydrolase family 31, GH31 uncultured microorganism
physiological function alpha-xylosidase is important for the degradation of oligoxyloglucan saccharides uncultured microorganism

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.086
-
 Isoprimeverose pH 5.5, 45°C, recombinant enzyme uncultured microorganism
64.4
-
 4-nitrophenyl alpha-D-xylopyranoside pH 5.5, 45°C, recombinant enzyme uncultured microorganism