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Literature summary for 3.2.1.176 extracted from

  • Schiano-di-Cola, C.; Rojel, N.; Jensen, K.; Kari, J.; Sorensen, T.H.; Borch, K.; Westh, P.
    Systematic deletions in the cellobiohydrolase (CBH) Cel7A from the fungus Trichoderma reesei reveal flexible loops critical for CBH activity (2019), J. Biol. Chem., 294, 1807-1815 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.176

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Aspergillus oryzae Trichoderma reesei

Inhibitors

Inhibitors Comment Organism Structure
cellobiose competitive inhibition Trichoderma reesei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.089
-
 4-nitrophenyl beta-lactoside at pH 5.0 and 25°C Trichoderma reesei

Organism

Organism UniProt Comment Textmining
Trichoderma reesei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Trichoderma reesei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-lactoside + H2O
-
 Trichoderma reesei 4-nitrophenol + lactose
-
 ?
avicel + H2O
-
 Trichoderma reesei ?
-
 ?
azurine-cross-linked hydroxyethylcellulose + H2O
-
 Trichoderma reesei ?
-
 ?
bacterial microcrystalline cellulose + H2O
-
 Trichoderma reesei ?
-
 ?
regenerated amorphous cellulose + H2O
-
 Trichoderma reesei ?
-
 ?

Synonyms

Synonyms Comment Organism
CBH
-
 Trichoderma reesei
Cel7A
-
 Trichoderma reesei
cellobiohydrolase I
-
 Trichoderma reesei

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
66.9
-
 melting temperature Trichoderma reesei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.78
-
 4-nitrophenyl beta-lactoside at pH 5.0 and 25°C Trichoderma reesei

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.021
-
 cellobiose at pH 5.0 and 25°C Trichoderma reesei