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Literature summary for 3.2.1.176 extracted from
- Expression of an endoglucanase-cellobiohydrolase fusion protein in Saccharomyces cerevisiae, Yarrowia lipolytica, and Lipomyces starkeyi (2018), Biotechnol. Biofuels, 11, 322 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed as an endoglucanase-cellobiohydrolase fusion protein in Saccharomyces cerevisiae, Yarrowia lipolytica, and Lipomyces starkeyi | Trichoderma reesei |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trichoderma reesei | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, phenyl Sepharose 6 column chromatography Source 15Q resin column chromatography, and Superdex 75 gel filtration | Trichoderma reesei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-cellobioside + H2O | minimal activity | Trichoderma reesei | 4-nitrophenol + cellobiose | - |
? |  |
| 4-nitrophenyl beta-D-lactoside + H2O | - |
Trichoderma reesei | 4-nitrophenol + D-lactose | - |
? | |
| acid-pretreated corn stover + H2O | - |
Trichoderma reesei | ? | - |
? | |
| avicel + H2O | - |
Trichoderma reesei | ? | - |
? | |
| cellulose + H2O | - |
Trichoderma reesei | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 39000, endoglucanase-cellobiohydrolase fusion protein, SDS-PAGE | Trichoderma reesei |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CBHI | - |
Trichoderma reesei |
| cellobiohydrolase I | - |
Trichoderma reesei |
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Release 2023.1 (January 2023)
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