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Literature summary for 3.2.1.176 extracted from
- Improving the thermal stability of cellobiohydrolase Cel7A from Hypocrea jecorina by directed evolution (2017), J. Biol. Chem., 292, 17418-17430 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Aspergillus niger variant awamori AP4 | Trichoderma reesei |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| sitting drop vapor diffusion method, using 25.5% (w/v) PEG 4000, 0.17 M ammonium sulfate, and 15% (v/v) glycerol | Trichoderma reesei |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| G22D/N49S/A68T/P227L/S278P/T296P | the mutant shows an increase in melting temperature and a greater half-life compared with the wild type enzyme | Trichoderma reesei |
| S8P/G22D/T41I/N49S/A68T/S113N/P227L /D249K/S278P/T296P/N301R | the mutant shows an increase in melting temperature and a greater half-life compared with the wild type enzyme | Trichoderma reesei |
| S8P/T41I/N49S/A68T/N89D/S92T/S113N /S196T/P227L/D249K/T255P/S278P/E295K /T296P/T332Y/V403D/S411F/T462I | the mutant, which is active up to 75°C, shows a 10.4°C increase in melting temperature and a 44fold greater half-life compared with the wild type enzyme | Trichoderma reesei |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellulose + H2O | Trichoderma reesei | - |
cellobiose + ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trichoderma reesei | P62694 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation and phenyl Sepharose resin column chromatography | Trichoderma reesei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl beta-lactoside + H2O | - |
Trichoderma reesei | 4-methylumbelliferol + beta-lactose | - |
? | |
| cellulose + H2O | - |
Trichoderma reesei | cellobiose + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Cel7A | - |
Trichoderma reesei |
| cellobiohydrolase | - |
Trichoderma reesei |
| cellobiohydrolase I | - |
Trichoderma reesei |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 53 | - |
- |
Trichoderma reesei |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 65 | the activity is highest at 53°C and slightly lower at 65°C, whereas the enzyme is practically inactive at 75°C | Trichoderma reesei |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 62 | 69 | the melting temperature is 62.5°C. The wild type enzyme shows half-lives of 19.9, 9.3 and 8.6 min at 62°C, 66°C, and 69°C, respectively | Trichoderma reesei |
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Release 2023.1 (January 2023)
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