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Literature summary for 3.2.1.176 extracted from
- Cloning of a gene encoding thermostable cellobiohydrolase from the thermophilic fungus Chaetomium thermophilum and its expression in Pichia pastoris (2009), J. Appl. Microbiol., 106, 1867-1875 .
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Pichia pastoris | Thermochaetoides thermophila |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 970 | - |
4-nitrophenyl beta-D-cellobioside | pH 5.0, 50°C | Thermochaetoides thermophila |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermochaetoides thermophila | A3F8V2 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | 1.3% carbohydrate content | Thermochaetoides thermophila |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-cellobioside + H2O | - |
Thermochaetoides thermophila | 4-nitrophenol + D-cellobiose | - |
? | |
| avicel + H2O | - |
Thermochaetoides thermophila | ? | - |
? | |
| filter paper + H2O | - |
Thermochaetoides thermophila | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 48000, SDS-PAGE, x * 476360, calculated | Thermochaetoides thermophila |
| More | mature protein CBH3 only comprises a catalytic domain and lacks cellulose-binding domain and a hinge region | Thermochaetoides thermophila |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Cbh3 | - |
Thermochaetoides thermophila |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Thermochaetoides thermophila |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
60 min, 60% residual activity | Thermochaetoides thermophila |
| 80 | - |
half-life 10 min | Thermochaetoides thermophila |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Thermochaetoides thermophila |
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Release 2023.1 (January 2023)
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