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Literature summary for 3.2.1.176 extracted from

  • Wei, H.; Wang, W.; Alahuhta, M.; Vander, Wall, T.; Baker, J.O.; Taylor, L.E. 2nd; Decker, S.R.; Himmel, M.E.; Zhang, M.
    Engineering towards a complete heterologous cellulase secretome in Yarrowia lipolytica reveals its potential for consolidated bioprocessing (2014), Biotechnol. Biofuels, 7, 148.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.176

Application

Application Comment Organism
biofuel production successful expression of a chimeric cellobiohydrolase I with essentially full native activity in Yarrowia lipolytica. Yarrowia lipolytica strains can be genetically engineered, ultimately by heterologous expression of fungal cellulases and other enzymes, to directly convert lignocellulosic substrates to biofuels Trichoderma reesei
biofuel production successful expression of a chimeric cellobiohydrolase I with essentially full native activity in Yarrowia lipolytica. Yarrowia lipolytica strains can be genetically engineered, ultimately by heterologous expression of fungal cellulases and other enzymes, to directly convert lignocellulosic substrates to biofuels Rasamsonia emersonii

Cloned(Commentary)

Cloned (Comment) Organism
folding and/or post-translation modification of heterologous enzyme in Yarrowia lipolytica cannot completely mimic that in the original source strain. Trichoderma reesei/Talatomyces emersonii chimeric cellobiohydrolase I containing the catalytic domain from Talaromyces emersonii and the linker and carbohydrate-binding module from Trichoderma reesei Trichoderma reesei
folding and/or post-translation modification of heterologous enzyme in Yarrowia lipolytica cannot completely mimic that in the original source strain. Trichoderma reesei/Talatomyces emersonii chimeric cellobiohydrolase I containing the catalytic domain from Talaromyces emersonii and the linker and carbohydrate-binding module from Trichoderma reesei Rasamsonia emersonii

Protein Variants

Protein Variants Comment Organism
additional information expression of Trichoderma reesei/Talatomyces emersonii chimeric cellobiohydrolase I containing the catalytic domain from Talaromyces emersonii and the linker and carbohydrate-binding module from Trichoderma reesei in Yarrowia lipolytica Trichoderma reesei
additional information expression of Trichoderma reesei/Talatomyces emersonii chimeric cellobiohydrolase I containing the catalytic domain from Talaromyces emersonii and the linker and carbohydrate-binding module from Trichoderma reesei in Yarrowia lipolytica Rasamsonia emersonii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 native enzyme produced in Trichoderma reesei, deglycosylated form (with Endo H treatment), SDS-PAGE Trichoderma reesei
53000
-
 Trichoderma reesei/Talatomyces emersonii chimeric cellobiohydrolase I containing the catalytic domain from Talaromyces emersonii and the linker and carbohydrate-binding module from Trichoderma reesei, deglycosylated form (with Endo H treatment), SDS-PAGE Trichoderma reesei
53000
-
 Trichoderma reesei/Talatomyces emersonii chimeric cellobiohydrolase I containing the catalytic domain from Talaromyces emersonii and the linker and carbohydrate-binding module from Trichoderma reesei, deglycosylated form (with Endo H treatment), SDS-PAGE Rasamsonia emersonii
61000
-
 native enzyme produced in Trichoderma reesei, glycosylated form (without Endo H treatment), SDS-PAGE Trichoderma reesei
63000
-
 Trichoderma reesei/Talatomyces emersonii chimeric cellobiohydrolase I containing the catalytic domain from Talaromyces emersonii and the linker and carbohydrate-binding module from Trichoderma reesei, glycosylated form (without Endo H treatment), SDS-PAGE Trichoderma reesei
63000
-
 Trichoderma reesei/Talatomyces emersonii chimeric cellobiohydrolase I containing the catalytic domain from Talaromyces emersonii and the linker and carbohydrate-binding module from Trichoderma reesei, glycosylated form (without Endo H treatment), SDS-PAGE Rasamsonia emersonii

Organism

Organism UniProt Comment Textmining
Rasamsonia emersonii Q8TFL9
-
-
Trichoderma reesei P62694 i.e. Hypocrea jecorina
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Rasamsonia emersonii
glycoprotein in the case of Trichoderma reesei/Talatomyces emersonii chimeric cellobiohydrolase I containing the catalytic domain from Talaromyces emersonii and the linker and carbohydrate-binding module from Trichoderma reesei which is expressed from Yarrowia, the overall magnitude of glycosylation is similar or only slightly higher than that for native cellobiohydrolase I produced in Trichoderma reesei Trichoderma reesei

Purification (Commentary)

Purification (Comment) Organism
-
 Trichoderma reesei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
avicel + H2O
-
 Trichoderma reesei cellobiose + D-glucose
-
 ?
avicel + H2O
-
 Rasamsonia emersonii cellobiose + D-glucose
-
 ?

Synonyms

Synonyms Comment Organism
cellobiohydrolase I
-
 Trichoderma reesei
cellobiohydrolase I
-
 Rasamsonia emersonii