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Literature summary for 3.2.1.176 extracted from

  • Shibafuji, Y.; Nakamura, A.; Uchihashi, T.; Sugimoto, N.; Fukuda, S.; Watanabe, H.; Samejima, M.; Ando, T.; Noji, H.; Koivula, A.; Igarashi, K.; Iino, R.
    Single-molecule imaging analysis of elementary reaction steps of Trichoderma reesei cellobiohydrolase I (Cel7A) hydrolyzing crystalline cellulose Ialpha and IIII (2014), J. Biol. Chem., 289, 14056-14065.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.176

Protein Variants

Protein Variants Comment Organism
S128C mutant generated for single-molecule fluorescence imaging analysis, shows hydrolysis activity comparable with those of the wild-type against cellulose Ialpha and IIII and similar dependence on the enzyme concentration Trichoderma reesei

Organism

Organism UniProt Comment Textmining
Trichoderma reesei G0RVK1
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-
Trichoderma reesei QM6a G0RVK1
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellulose Ialpha + H2O different susceptibilities of cellulose Ialpha and IIII are highly dependent on enzyme concentration, and at nanomolar enzyme concentration, Cel7A shows similar rates of hydrolysis against cellulose Ialpha and IIII. At picomolar enzyme concentration, Cel7A displays similar binding and dissociation rate constants for cellulose Ialpha and IIII and similar fractions of productive binding. Once productively bound, Cel7A processively hydrolyzes and moves along cellulose Ialpha and IIII with similar translational rates Trichoderma reesei cellobiose + ?
-
 ?
cellulose Ialpha + H2O different susceptibilities of cellulose Ialpha and IIII are highly dependent on enzyme concentration, and at nanomolar enzyme concentration, Cel7A shows similar rates of hydrolysis against cellulose Ialpha and IIII. At picomolar enzyme concentration, Cel7A displays similar binding and dissociation rate constants for cellulose Ialpha and IIII and similar fractions of productive binding. Once productively bound, Cel7A processively hydrolyzes and moves along cellulose Ialpha and IIII with similar translational rates Trichoderma reesei QM6a cellobiose + ?
-
 ?
cellulose IIII + H2O different susceptibilities of cellulose Ialpha and IIII are highly dependent on enzyme concentration, and at nanomolar enzyme concentration, Cel7A shows similar rates of hydrolysis against cellulose Ialpha and IIII. At picomolar enzyme concentration, Cel7A displays similar binding and dissociation rate constants for cellulose Ialpha and IIII and similar fractions of productive binding. Once productively bound, Cel7A processively hydrolyzes and moves along cellulose Ialpha and IIII with similar translational rates Trichoderma reesei cellobiose + ?
-
 ?
cellulose IIII + H2O different susceptibilities of cellulose Ialpha and IIII are highly dependent on enzyme concentration, and at nanomolar enzyme concentration, Cel7A shows similar rates of hydrolysis against cellulose Ialpha and IIII. At picomolar enzyme concentration, Cel7A displays similar binding and dissociation rate constants for cellulose Ialpha and IIII and similar fractions of productive binding. Once productively bound, Cel7A processively hydrolyzes and moves along cellulose Ialpha and IIII with similar translational rates Trichoderma reesei QM6a cellobiose + ?
-
 ?